- Although the use of stable transformation technology has led to great insight into gene function, its application in high-throughput studies remains arduous. Agro-infiltration have been widely used in species such as Nicotiana benthamiana for the rapid detection of gene expression and protein interaction analysis, but this technique does not work efficiently in other plant species, including Arabidopsis thaliana. As an efficient high-throughput transient expression system is currently lacking in the model plant species A. thaliana, we developed a method that is characterized by high efficiency, reproducibility, and suitability for transient expression of a variety of functional proteins in A. thaliana and 7 other plant species, including Brassica oleracea, Capsella rubella, Thellungiella salsuginea, Thellungiella halophila, Solanum tuberosum, Capsicum annuum, and N. benthamiana. Efficiency of this method was independently verified in three independent research facilities, pointing to the robustness of this technique. Furthermore, inAlthough the use of stable transformation technology has led to great insight into gene function, its application in high-throughput studies remains arduous. Agro-infiltration have been widely used in species such as Nicotiana benthamiana for the rapid detection of gene expression and protein interaction analysis, but this technique does not work efficiently in other plant species, including Arabidopsis thaliana. As an efficient high-throughput transient expression system is currently lacking in the model plant species A. thaliana, we developed a method that is characterized by high efficiency, reproducibility, and suitability for transient expression of a variety of functional proteins in A. thaliana and 7 other plant species, including Brassica oleracea, Capsella rubella, Thellungiella salsuginea, Thellungiella halophila, Solanum tuberosum, Capsicum annuum, and N. benthamiana. Efficiency of this method was independently verified in three independent research facilities, pointing to the robustness of this technique. Furthermore, in addition to demonstrating the utility of this technique in a range of species, we also present a case study employing this method to assess protein-protein interactions in the sucrose biosynthesis pathway in Arabidopsis.…
MetadatenAuthor details: | Youjun ZhangGND, Moxian Chen, Beata SiemiatkowskaORCiDGND, Mitchell Rey Toleco, Yue Jing, Vivien StrotmannORCiD, Jianghua Zhang, Yvonne StahlGND, Alisdair FernieORCiDGND |
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URN: | urn:nbn:de:kobv:517-opus4-524254 |
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DOI: | https://doi.org/10.25932/publishup-52425 |
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ISSN: | 1866-8372 |
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Title of parent work (German): | Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe |
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Publication series (Volume number): | Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe (1189) |
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Publication type: | Postprint |
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Language: | English |
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Date of first publication: | 2019/09/04 |
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Publication year: | 2020 |
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Publishing institution: | Universität Potsdam |
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Release date: | 2021/11/03 |
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Tag: | agro-infiltration; protein-protein interaction; subcellular localization; transient expression |
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Issue: | 5 |
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Article number: | 100028 |
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Number of pages: | 14 |
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Source: | Plant Communications, Volume 1, Issue 5, 2020, 100028, ISSN 2590-3462, https://doi.org/10.1016/j.xplc.2020.100028 |
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Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
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DDC classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
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Peer review: | Referiert |
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Publishing method: | Open Access / Green Open-Access |
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License (German): | CC-BY-NC-ND - Namensnennung, nicht kommerziell, keine Bearbeitungen 4.0 International |
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External remark: | Bibliographieeintrag der Originalveröffentlichung/Quelle |
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