Synthesis of membrane proteins in eukaryotic cell-free systems
- Cell-free protein synthesis (CFPS) is a valuable method for the fast expression of difficult-to-express proteins as well as posttranslationally modified proteins. Since cell-free systems circumvent possible cytotoxic effects caused by protein overexpression in living cells, they significantly enlarge the scale and variety of proteins that can be characterized. We demonstrate the high potential of eukaryotic CFPS to express various types of membrane proteins covering a broad range of structurally and functionally diverse proteins. Our eukaryotic cell-free translation systems are capable to provide high molecular weight membrane proteins, fluorescent-labeled membrane proteins, as well as posttranslationally modified proteins for further downstream analysis.
| Author details: | Rita Sachse, Doreen Anja Wüstenhagen, Maria Samalikova, Michael Gerrits, Frank Fabian BierORCiDGND, Stefan KubickORCiD |
|---|---|
| DOI: | https://doi.org/10.1002/elsc.201100235 |
| ISSN: | 1618-0240 |
| Title of parent work (English): | Engineering in life sciences : Industry, Environment, Plant, Food |
| Publisher: | Wiley-Blackwell |
| Place of publishing: | Hoboken |
| Publication type: | Article |
| Language: | English |
| Year of first publication: | 2013 |
| Publication year: | 2013 |
| Release date: | 2017/03/26 |
| Tag: | Cell-free protein expression; In vitro protein synthesis; Labeled membrane proteins; Synthetic glycoprotein |
| Volume: | 13 |
| Issue: | 1 |
| Number of pages: | 10 |
| First page: | 39 |
| Last Page: | 48 |
| Funding institution: | German Ministry of Education and Research (BMBF) [0313965A, 0312039] |
| Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
| Peer review: | Referiert |
