Dictyostelium spastin is involved in nuclear envelope dynamics during semi-closed mitosis
- Dictyostelium amoebae perform a semi-closed mitosis, in which the nuclear envelope is fenestrated at the insertion sites of the mitotic centrosomes and around the central spindle during karyokinesis. During late telophase the centrosome relocates to the cytoplasmic side of the nucleus, the central spindle disassembles and the nuclear fenestrae become closed. Our data indicate that Dictyostelium spastin (DdSpastin) is a microtubule-binding and severing type I membrane protein that plays a role in this process. Its mitotic localization is in agreement with a requirement for the removal of microtubules that would hinder closure of the fenestrae. Furthermore, DdSpastin interacts with the HeH/ LEM-family protein Src1 in BioID analyses as well as the inner nuclear membrane protein Sun1, and shows subcellular co-localizations with Src1, Sun1, the ESCRT component CHMP7 and the IST1-like protein filactin, suggesting that the principal pathway of mitotic nuclear envelope remodeling is conserved between animals and Dictyostelium amoebae.
Author details: | Ulrike Schweigel, Petros BatsiosGND, Annette Müller-TaubenbergerORCiDGND, Ralph GräfORCiDGND, Marianne GrafeORCiDGND |
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DOI: | https://doi.org/10.1080/19491034.2022.2047289 |
ISSN: | 1949-1034 |
ISSN: | 1949-1042 |
Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/35298348 |
Title of parent work (English): | Nucleus |
Publisher: | Taylor & Francis Group |
Place of publishing: | Philadelphia |
Publication type: | Article |
Language: | English |
Date of first publication: | 2022/03/17 |
Publication year: | 2022 |
Release date: | 2023/05/15 |
Tag: | ESCRT; LEM-domain; Spastin; dictyostelium; mitosis; nuclear envelope; sun1 |
Volume: | 13 |
Issue: | 1 |
Number of pages: | 11 |
First page: | 144 |
Last Page: | 154 |
Funding institution: | Deutsche Forschungsgemeinschaft [GR1642/9-1] |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
DDC classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Peer review: | Referiert |
Publishing method: | Open Access / Gold Open-Access |
DOAJ gelistet | |
License (German): | CC-BY - Namensnennung 4.0 International |