Astrid Näther, Bärbel U. Fösel, Verena Nägele, Pia K. Wüst, Jan Weinert, Michael Bonkowski, Fabian Alt, Yvonne Oelmann, Andrea Polle, Gertrud Lohaus, Sonja Gockel, Andreas Hemp, Elisabeth K. V. Kalko, Karl Eduard Linsenmair, Simone Pfeiffer, Swen Renner, Ingo Schöning, Wolfgang W. Weisser, Konstans Wells, Markus Fischer, Jörg Overmann, Michael W. Friedrich
- In soil, Acidobacteria constitute on average 20% of all bacteria, are highly diverse, and are physiologically active in situ. However, their individual functions and interactions with higher taxa in soil are still unknown. Here, potential effects of land use, soil properties, plant diversity, and soil nanofauna on acidobacterial community composition were studied by cultivation-independent methods in grassland and forest soils from three different regions in Germany. The analysis of 16S rRNA gene clone libraries representing all studied soils revealed that grassland soils were dominated by subgroup Gp6 and forest soils by subgroup Gp1 Acidobacteria. The analysis of a large number of sites (n = 57) by 16S rRNA gene fingerprinting methods (terminal restriction fragment length polymorphism [T-RFLP] and denaturing gradient gel electrophoresis [DGGE]) showed that Acidobacteria diversities differed between grassland and forest soils but also among the three different regions. Edaphic properties, such as pH, organic carbon, total nitrogen,In soil, Acidobacteria constitute on average 20% of all bacteria, are highly diverse, and are physiologically active in situ. However, their individual functions and interactions with higher taxa in soil are still unknown. Here, potential effects of land use, soil properties, plant diversity, and soil nanofauna on acidobacterial community composition were studied by cultivation-independent methods in grassland and forest soils from three different regions in Germany. The analysis of 16S rRNA gene clone libraries representing all studied soils revealed that grassland soils were dominated by subgroup Gp6 and forest soils by subgroup Gp1 Acidobacteria. The analysis of a large number of sites (n = 57) by 16S rRNA gene fingerprinting methods (terminal restriction fragment length polymorphism [T-RFLP] and denaturing gradient gel electrophoresis [DGGE]) showed that Acidobacteria diversities differed between grassland and forest soils but also among the three different regions. Edaphic properties, such as pH, organic carbon, total nitrogen, C/N ratio, phosphorus, nitrate, ammonium, soil moisture, soil temperature, and soil respiration, had an impact on community composition as assessed by fingerprinting. However, interrelations with environmental parameters among subgroup terminal restriction fragments (T-RFs) differed significantly, e.g., different Gp1 T-RFs correlated positively or negatively with nitrogen content. Novel significant correlations of Acidobacteria subpopulations (i.e., individual populations within subgroups) with soil nanofauna and vascular plant diversity were revealed only by analysis of clone sequences. Thus, for detecting novel interrelations of environmental parameters with Acidobacteria, individual populations within subgroups have to be considered.…
MetadatenAuthor details: | Astrid Näther, Bärbel U. Fösel, Verena Nägele, Pia K. Wüst, Jan Weinert, Michael Bonkowski, Fabian Alt, Yvonne Oelmann, Andrea Polle, Gertrud Lohaus, Sonja Gockel, Andreas Hemp, Elisabeth K. V. Kalko, Karl Eduard Linsenmair, Simone Pfeiffer, Swen Renner, Ingo Schöning, Wolfgang W. Weisser, Konstans Wells, Markus Fischer, Jörg OvermannGND, Michael W. Friedrich |
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DOI: | https://doi.org/10.1128/AEM.01325-12 |
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ISSN: | 0099-2240 |
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Title of parent work (English): | Applied and environmental microbiology |
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Publisher: | American Society for Microbiology |
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Place of publishing: | Washington |
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Publication type: | Article |
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Language: | English |
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Year of first publication: | 2012 |
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Publication year: | 2012 |
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Release date: | 2017/03/26 |
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Volume: | 78 |
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Issue: | 20 |
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Number of pages: | 9 |
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First page: | 7398 |
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Last Page: | 7406 |
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Funding institution: | DFG Priority Program [1374, OV 20/18-1, -18-2, FR 1151/5-1, -5-2]; Max
Planck Society (Munich); University of Bremen |
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Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
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Peer review: | Referiert |
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