Arsenic-containing hydrocarbons disrupt a model in vitro blood-cerebrospinal fluid barrier
- Lipid-soluble arsenicals, so-called arsenolipids, have gained a lot of attention in the last few years because of their presence in many seafoods and reports showing substantial cytotoxicity emanating from arsenic-containing hydrocarbons (AsHCs), a prominent subgroup of the arsenolipids. More recent in vivo and in vitro studies indicate that some arsenolipids might have adverse effects on brain health. In the present study, we focused on the effects of selected arsenolipids and three representative metabolites on the blood-cerebrospinal fluid barrier (B-CSF-B), a brain-regulating interface. For this purpose, we incubated an in vitro model of the B-CSF-B composed of porcine choroid plexus epithelial cells (PCPECs) with three AsHCs, two arsenic-containing fatty acids (AsFAs) and three representative arsenolipid metabolites (dimethylarsinic acid, thio/oxo-dimethylpropanoic acid) to examine their cytotoxic potential and impact on barrier integrity. The toxic arsenic species arsenite was also tested in this way and served as a referenceLipid-soluble arsenicals, so-called arsenolipids, have gained a lot of attention in the last few years because of their presence in many seafoods and reports showing substantial cytotoxicity emanating from arsenic-containing hydrocarbons (AsHCs), a prominent subgroup of the arsenolipids. More recent in vivo and in vitro studies indicate that some arsenolipids might have adverse effects on brain health. In the present study, we focused on the effects of selected arsenolipids and three representative metabolites on the blood-cerebrospinal fluid barrier (B-CSF-B), a brain-regulating interface. For this purpose, we incubated an in vitro model of the B-CSF-B composed of porcine choroid plexus epithelial cells (PCPECs) with three AsHCs, two arsenic-containing fatty acids (AsFAs) and three representative arsenolipid metabolites (dimethylarsinic acid, thio/oxo-dimethylpropanoic acid) to examine their cytotoxic potential and impact on barrier integrity. The toxic arsenic species arsenite was also tested in this way and served as a reference substance. While AsFAs and the metabolites showed no cytotoxic effects in the conducted assays, AsHCs showed a strong cytotoxicity, being up to 1.5-fold more cytotoxic than arsenite. Analysis of the in vitro B-CSF-B integrity showed a concentration dependent disruption of the barrier within 72 h. The correlation with the decreased plasma membrane surface area (measured as capacitance) indicates cytotoxic effects. These findings suggest exposure to elevated levels of certain arsenolipids may have detrimental consequences for the central nervous system.…
Verfasserangaben: | Sandra Marie MüllerORCiDGND, Franziska EbertORCiDGND, Julia BornhorstORCiDGND, Hans-Joachim GallaORCiD, Kevin A. FrancesconiORCiD, Tanja SchwerdtleORCiDGND |
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DOI: | https://doi.org/10.1016/j.jtemb.2018.01.020 |
ISSN: | 0946-672X |
Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/29449109 |
Titel des übergeordneten Werks (Englisch): | Journal of trace elements in medicine and biology |
Verlag: | Elsevier GMBH |
Verlagsort: | München |
Publikationstyp: | Wissenschaftlicher Artikel |
Sprache: | Englisch |
Datum der Erstveröffentlichung: | 15.06.2018 |
Erscheinungsjahr: | 2018 |
Datum der Freischaltung: | 21.03.2022 |
Freies Schlagwort / Tag: | Arsenic-containing fatty acids; Arsenic-containing hydrocarbons; Arsenolipids; Blood-cerebrospinal fluid barrier; Blood-liquor barrier |
Band: | 49 |
Seitenanzahl: | 7 |
Erste Seite: | 171 |
Letzte Seite: | 177 |
Fördernde Institution: | Heinrich-Stockmeyer-Foundation; German Research Foundation (DFG)German Research Foundation (DFG) [SCHW903/10-1]; Austrian Science Fund (FWF)Austrian Science Fund (FWF) [I2412-B21] |
Organisationseinheiten: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Ernährungswissenschaft |
DDC-Klassifikation: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |