Molecular epidemiology of methicillin-susceptible and methicillin-resistant Staphylococcus aureus in wild, captive and laboratory rats
- Rats are a reservoir of human- and livestock-associated methicillin-resistant Staphylococcus aureus (MRSA). However, the composition of the natural S. aureus population in wild and laboratory rats is largely unknown. Here, 144 nasal S. aureus isolates from free-living wild rats, captive wild rats and laboratory rats were genotyped and profiled for antibiotic resistances and human-specific virulence genes. The nasal S. aureus carriage rate was higher among wild rats (23.4%) than laboratory rats (12.3%). Free-living wild rats were primarily colonized with isolates of clonal complex (CC) 49 and CC130 and maintained these strains even in husbandry. Moreover, upon livestock contact, CC398 isolates were acquired. In contrast, laboratory rats were colonized with many different S. aureus lineages—many of which are commonly found in humans. Five captive wild rats were colonized with CC398-MRSA. Moreover, a single CC30-MRSA and two CC130-MRSA were detected in free-living or captive wild rats. Rat-derived S. aureus isolates rarely harbored theRats are a reservoir of human- and livestock-associated methicillin-resistant Staphylococcus aureus (MRSA). However, the composition of the natural S. aureus population in wild and laboratory rats is largely unknown. Here, 144 nasal S. aureus isolates from free-living wild rats, captive wild rats and laboratory rats were genotyped and profiled for antibiotic resistances and human-specific virulence genes. The nasal S. aureus carriage rate was higher among wild rats (23.4%) than laboratory rats (12.3%). Free-living wild rats were primarily colonized with isolates of clonal complex (CC) 49 and CC130 and maintained these strains even in husbandry. Moreover, upon livestock contact, CC398 isolates were acquired. In contrast, laboratory rats were colonized with many different S. aureus lineages—many of which are commonly found in humans. Five captive wild rats were colonized with CC398-MRSA. Moreover, a single CC30-MRSA and two CC130-MRSA were detected in free-living or captive wild rats. Rat-derived S. aureus isolates rarely harbored the phage-carried immune evasion gene cluster or superantigen genes, suggesting long-term adaptation to their host. Taken together, our study revealed a natural S. aureus population in wild rats, as well as a colonization pressure on wild and laboratory rats by exposure to livestock- and human-associated S. aureus, respectively.…
Author details: | Dina RaafatORCiD, Daniel M. MrochenORCiD, Fawaz Al’Sholui, Elisa Heuser, René RyllGND, Kathleen R. Pritchett-CorningORCiDGND, Jens JacobORCiDGND, Bernd WaltherORCiDGND, Franz-Rainer MatuschkaGND, Dania RichterORCiDGND |
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URN: | urn:nbn:de:kobv:517-opus4-512379 |
DOI: | https://doi.org/10.25932/publishup-51237 |
ISSN: | 1866-8364 |
Title of parent work (German): | Zweitveröffentlichungen der Universität Potsdam : Humanwissenschaftliche Reihe |
Subtitle (English): | Effect of habitat on the nasal S. aureus population |
Publication series (Volume number): | Zweitveröffentlichungen der Universität Potsdam : Humanwissenschaftliche Reihe (873) |
Publication type: | Postprint |
Language: | English |
Date of first publication: | 2020/01/24 |
Publication year: | 2020 |
Publishing institution: | Universität Potsdam |
Release date: | 2024/04/22 |
Tag: | clonal complex; coagulation; epidemiology; habitat; host adaptation; immune evasion cluster; laboratory; livestock; rat Staphylococcus aureus |
Issue: | 2 |
Article number: | 80 |
Number of pages: | 24 |
Source: | Toxins 2020, 12(2), 80; https://doi.org/10.3390/toxins12020080 |
Organizational units: | Humanwissenschaftliche Fakultät |
DDC classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Peer review: | Referiert |
Publishing method: | Open Access / Green Open-Access |
License (German): | CC-BY - Namensnennung 4.0 International |
External remark: | Bibliographieeintrag der Originalveröffentlichung/Quelle |