Improvement of a fluorescence immunoassay with a compact diode-pumped solid state laser at 315 nm

  • We demonstrate the improvement of fluorescence immunoassay (FIA) diagnostics in deploying a newly developed compact diode-pumped solid state (DPSS) laser with emission at 315 nm. The laser is based on the quasi-three-level transition in Nd:YAG at 946 nm. The pulsed operation is either realized by an active Q-switch using an electro-optical device or by introduction of a Cr<SUP>4+</SUP>:YAG saturable absorber as passive Q-switch element. By extra-cavity second harmonic generation in different nonlinear crystal media we obtained blue light at 473 nm. Subsequent mixing of the fundamental and the second harmonic in a β-barium-borate crystal provided pulsed emission at 315 nm with up to 20 μJ maximum pulse energy and 17 ns pulse duration. Substitution of a nitrogen laser in a FIA diagnostics system by the DPSS laser succeeded in considerable improvement of the detection limit. Despite significantly lower pulse energies (7 μJ DPSS laser versus 150 μJ nitrogen laser), in preliminary investigations the limit of detection was reduced by a factWe demonstrate the improvement of fluorescence immunoassay (FIA) diagnostics in deploying a newly developed compact diode-pumped solid state (DPSS) laser with emission at 315 nm. The laser is based on the quasi-three-level transition in Nd:YAG at 946 nm. The pulsed operation is either realized by an active Q-switch using an electro-optical device or by introduction of a Cr<SUP>4+</SUP>:YAG saturable absorber as passive Q-switch element. By extra-cavity second harmonic generation in different nonlinear crystal media we obtained blue light at 473 nm. Subsequent mixing of the fundamental and the second harmonic in a β-barium-borate crystal provided pulsed emission at 315 nm with up to 20 μJ maximum pulse energy and 17 ns pulse duration. Substitution of a nitrogen laser in a FIA diagnostics system by the DPSS laser succeeded in considerable improvement of the detection limit. Despite significantly lower pulse energies (7 μJ DPSS laser versus 150 μJ nitrogen laser), in preliminary investigations the limit of detection was reduced by a factor of three for a typical FIA.show moreshow less

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Metadaten
Author:Matthias Niederkrüger, Christian Salb, Michael Beck, Niko Hildebrandt, Hans-Gerd Löhmannsröben, Gerd Marowsky
URN:urn:nbn:de:kobv:517-opus-10150
Series (Serial Number):Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe (paper 016)
Document Type:Postprint
Language:English
Date of Publication (online):2006/11/01
Year of Completion:2006
Publishing Institution:Universität Potsdam
Release Date:2006/11/01
Tag:315 nm; 473 nm; 946 nm; gepulster DPSS Laser; sättigbarer Absorber
315 nm; 473 nm; 946 nm; fluorescence immunoassay; pulsed DPSS laser
GND Keyword:Fluoreszenz-Resonanz-Energie-Transfer; Immunoassay; Neodym-YAG-Laser
Source:Proc. SPIE, Vol. 6380 (2006) 63800M
RVK - Regensburg Classification:VE 9908
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie
Extern / Extern
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften
Collections:Universität Potsdam / Aufsätze (Pre- und Postprints) / Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie / Physikalische Chemie
Notes extern:
© 2006 Society of Photo-Optical Instrumentation Engineers

This paper was published in:
Smart Medical and Biomedical Sensor Technology IV / Brian M. Cullum, J. Chance Carter (eds.), Proceedings of SPIE, Vol. 6380 (2006)
ISBN: 0-8194-6478-3

It is made available as an electronic reprint with permission of SPIE. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purpose, or modification of the content of the paper are prohibited.