Products of in vitro oxidation of N-acetyldopamine as possible components in the sclerotization of insect cuticle

  • [1-14C]-N-Acetyldopamine (NADA) was oxidized in the presence of methyl [3-3H]-β-alanate with mushroom tyrosinase. The complex mixture of reaction products was partly resolved by chromatographic procedures and analyzed by spectroscopic methods. Methyl-β-alanate is incorporated to only a small extent into oxidation products of NADA which inter alia are presumed to be oligomeric hydroxyquinones. After oxidation of [1-14C, 2-3H]-NADA with preparations from tanning Manduca sexta pupal cuticle, N-acetylnoradrenalin was identified as one of the products. Binding of radioactivity to melanin-like material was also observed. These results suggest that oxidation products different from those formulated usually for the crosslinkages between protein amino groups and N-acetyldopaquinone are deposited in darkly brown coloured insect cuticles during sclerotization.

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Author:Martin G. Peter
Series (Serial Number):Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe (paper 041)
Document Type:Postprint
Year of Completion:1980
Publishing Institution:Universität Potsdam
Release Date:2008/03/14
Tag:N-acetyldopamine; sclerotization; tanning agents; tyrosinase; o-quinones
Source:Insect Biochemistry. - ISSN 0965-1748. - 10 (1980), 2, p. 221 - 227
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Licence (German):License LogoKeine Nutzungslizenz vergeben - es gilt das deutsche Urheberrecht
Notes extern:
first published in:
Insect Biochemistry - 10 (1980), 2 p. 221 - 227
ISSN: 0965-1748
doi: 10.1016/0020-1790(80)90077-3