Monitoring hormone-induced oxygen consumption in the salivary glands of the blowfly, Calliphora vicina, by use of luminescent microbeads

  • The salivary glands of the blowfly were injected with luminescent oxygen-sensitive microbeads. The changes in oxygen content within individual gland tubules during hormone-induced secretory activity were quantified. The measurements are based on an upgraded phase-modulation technique, where the phase shift of the sensor phosphorescence is determined independently from concentration and background signals. We show that the combination of a lock-in amplifier with a fluorescence microscope results in a convenient setup to measure oxygen concentrations within living animal tissues at the cellular level.

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Author:Elmar Schmälzlin, Bernd Walz, Ingo Klimant, Bettina Schewe, Hans-Gerd Löhmannsröben
Series (Serial Number):Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe (paper 006)
Document Type:Postprint
Date of Publication (online):2007/01/26
Year of Completion:2006
Publishing Institution:Universität Potsdam
Release Date:2007/01/26
Source:Sensors and Actuators B: Chemical. - ISSN 0925-4005. - 119 (2006), 1 , p. 251 - 254
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften
Collections:Universität Potsdam / Aufsätze (Pre- und Postprints) / Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie / Physikalische Chemie
Notes extern:
first published in:
Sensors and Actuators B: Chemical. - ISSN 0925-4005. - 119 (2006), 1, p. 251 - 254
doi: 10.1016/j.snb.2005.12.024