An optical multifrequency phase-modulation method using microbeads for measuring intracellular oxygen concentrations in plants

  • A technique has been developed to measure absolute intracellular oxygen concentrations in green plants. Oxygen-sensitive phosphorescent microbeads were injected into the cells and an optical multifrequency phase-modulation technique was used to discriminate the sensor signal from the strong autofluorescence of the plant tissue. The method was established using photosynthesis-competent cells of the giant algae Chara corallina L., and was validated by application to various cell types of other plant species.

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Author:Elmar Schmälzlin, Joost T. van Dongen, Ingo Klimant, Bettina Marmodée, Martin Steup, Joachim Fishahn, Peter Geigenberger, Hans-Gerd Löhmannsröben
Series (Serial Number):Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe (paper 007)
Document Type:Postprint
Year of Completion:2005
Publishing Institution:Universität Potsdam
Release Date:2007/01/26
Source:Biophysical Journal. - ISSN 1542-0086 (online). - 89 (2005), S. 1339 - 1345
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften
Notes extern:
first published in:
Biophysical Journal. - ISSN 1542-0086. - 89 (2005), S. 1339 - 1345
doi: 10.1529/biophysj.105.063453