TY  - JOUR
A1  - Liu, Qingting
A1  - Zhou, Yuan
A1  - Fettke, Jörg
T1  - Starch granule size and morphology of Arabidopsis thaliana starch-related mutants analyzed during diurnal rhythm and development
JF  - Molecules : a journal of synthetic chemistry and natural product chemistry / Molecular Diversity Preservation International
N2  - Transitory starch plays a central role in the life cycle of plants. Many aspects of this important metabolism remain unknown; however, starch granules provide insight into this persistent metabolic process. Therefore, monitoring alterations in starch granules with high temporal resolution provides one significant avenue to improve understanding. Here, a previously established method that combines LCSM and safranin-O staining for in vivo imaging of transitory starch granules in leaves of Arabidopsis thaliana was employed to demonstrate, for the first time, the alterations in starch granule size and morphology that occur both throughout the day and during leaf aging. Several starch-related mutants were included, which revealed differences among the generated granules. In ptst2 and sex1-8, the starch granules in old leaves were much larger than those in young leaves; however, the typical flattened discoid morphology was maintained. In ss4 and dpe2/phs1/ss4, the morphology of starch granules in young leaves was altered, with a more rounded shape observed. With leaf development, the starch granules became spherical exclusively in dpe2/phs1/ss4. Thus, the presented data provide new insights to contribute to the understanding of starch granule morphogenesis.
KW  - starch metabolism
KW  - starch granule
KW  - starch granule size
KW  - starch granule morphology
KW  - LCSM
KW  - Arabidopsis thaliana
Y1  - 2021
U6  - https://doi.org/10.3390/molecules26195859
SN  - 1420-3049
VL  - 26
SP  - 1
EP  - 9
PB  - MDPI
CY  - Basel, Schweiz
ET  - 19
ER  -