TY  - JOUR
A1  - Woodhouse, Jason Nicholas
A1  - Makower, A. Katharina
A1  - Yeung, Anna C. Y.
A1  - Ongley, Sarah E.
A1  - Micallef, Melinda L.
A1  - Moffitt, Michelle C.
A1  - Neilan, Brett A.
T1  - Advances in genomics, transcriptomics and proteomics of toxin-producing cyanobacteria
JF  - Environmental microbiology reports
N2  - A common misconception persists that the genomes of toxic and non-toxic cyanobacterial strains are largely conserved with the exception of the presence or absence of the genes responsible for toxin production. Implementation of -omics era technologies has challenged this paradigm, with comparative analyses providing increased insight into the differences between strains of the same species. The implementation of genomic, transcriptomic and proteomic approaches has revealed distinct profiles between toxin-producing and non-toxic strains. Further, metagenomics and metaproteomics highlight the genomic potential and functional state of toxic bloom events over time. In this review, we highlight how these technologies have shaped our understanding of the complex relationship between these molecules, their producers and the environment at large within which they persist.
Y1  - 2016
U6  - https://doi.org/10.1111/1758-2229.12366
SN  - 1758-2229
VL  - 8
SP  - 3
EP  - 13
PB  - Wiley-Blackwell
CY  - Hoboken
ER  - 
TY  - JOUR
A1  - Ilicic, Doris
A1  - Woodhouse, Jason
A1  - Karsten, Ulf
A1  - Zimmermann, Jonas
A1  - Wichard, Thomas
A1  - Quartino, Maria Liliana
A1  - Campana, Gabriela Laura
A1  - Livenets, Alexandra
A1  - Van den Wyngaert, Silke
A1  - Grossart, Hans-Peter
T1  - Antarctic Glacial Meltwater Impacts the Diversity of Fungal Parasites Associated With Benthic Diatoms in Shallow Coastal Zones
JF  - Frontiers in microbiology
N2  - Aquatic ecosystems are frequently overlooked as fungal habitats, although there is increasing evidence that their diversity and ecological importance are greater than previously considered. Aquatic fungi are critical and abundant components of nutrient cycling and food web dynamics, e.g., exerting top-down control on phytoplankton communities and forming symbioses with many marine microorganisms. However, their relevance for microphytobenthic communities is almost unexplored. In the light of global warming, polar regions face extreme changes in abiotic factors with a severe impact on biodiversity and ecosystem functioning. Therefore, this study aimed to describe, for the first time, fungal diversity in Antarctic benthic habitats along the salinity gradient and to determine the co-occurrence of fungal parasites with their algal hosts, which were dominated by benthic diatoms. Our results reveal that Ascomycota and Chytridiomycota are the most abundant fungal taxa in these habitats. We show that also in Antarctic waters, salinity has a major impact on shaping not just fungal but rather the whole eukaryotic community composition, with a diversity of aquatic fungi increasing as salinity decreases. Moreover, we determined correlations between putative fungal parasites and potential benthic diatom hosts, highlighting the need for further systematic analysis of fungal diversity along with studies on taxonomy and ecological roles of Chytridiomycota.
KW  - Antarctica
KW  - aquatic fungi
KW  - Chytridiomycota
KW  - phytoplankton host
KW  - salinity gradient
KW  - Illumina amplicon sequencing
KW  - Carlini Station
Y1  - 2022
U6  - https://doi.org/10.3389/fmicb.2022.805694
SN  - 1664-302X
IS  - 13
PB  - Frontiers Media
CY  - Lausanne
ER  - 
TY  - GEN
A1  - Hornick, Thomas
A1  - Bach, Lennart T.
A1  - Crawfurd, Katharine J.
A1  - Spilling, Kristian
A1  - Achterberg, Eric Pieter
A1  - Woodhouse, Jason Nicholas
A1  - Schulz, Kai Georg
A1  - Brussaard, Corina P. D.
A1  - Riebesell, Ulf
A1  - Grossart, Hans-Peter
T1  - Ocean acidification impacts bacteria–phytoplankton coupling at low-nutrient conditions
T2  - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - The oceans absorb about a quarter of the annually produced anthropogenic atmospheric carbon dioxide (CO2), resulting in a decrease in surface water pH, a process termed ocean acidification (OA). Surprisingly little is known about how OA affects the physiology of heterotrophic bacteria or the coupling of heterotrophic bacteria to phytoplankton when nutrients are limited. Previous experiments were, for the most part, undertaken during productive phases or following nutrient additions designed to stimulate algal blooms. Therefore, we performed an in situ large-volume mesocosm (similar to 55 m(3)) experiment in the Baltic Sea by simulating different fugacities of CO2 (fCO(2)) extending from present to future conditions. The study was conducted in July-August after the nominal spring bloom, in order to maintain low-nutrient conditions throughout the experiment. This resulted in phytoplankton communities dominated by small-sized functional groups (picophytoplankton). There was no consistent fCO(2)-induced effect on bacterial protein production (BPP), cell-specific BPP (csBPP) or biovolumes (BVs) of either free-living (FL) or particle-associated (PA) heterotrophic bacteria, when considered as individual components (univariate analyses). Permutational Multivariate Analysis of Variance (PERMANOVA) revealed a significant effect of the fCO(2) treatment on entire assemblages of dissolved and particulate nutrients, metabolic parameters and the bacteria-phytoplankton community. However, distance-based linear modelling only identified fCO(2) as a factor explaining the variability observed amongst the microbial community composition, but not for explaining variability within the metabolic parameters. This suggests that fCO(2) impacts on microbial metabolic parameters occurred indirectly through varying physicochemical parameters and microbial species composition. Cluster analyses examining the co-occurrence of different functional groups of bacteria and phytoplankton further revealed a separation of the four fCO(2)-treated mesocosms from both control mesocosms, indicating that complex trophic interactions might be altered in a future acidified ocean. Possible consequences for nutrient cycling and carbon export are still largely unknown, in particular in a nutrient-limited ocean.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 667 
KW  - northern Baltic Sea
KW  - inorganic nutrients
KW  - mesocosm experiment
KW  - elevated CO2
KW  - heterotrophic bacteria
KW  - organic-carbon
KW  - bacterioplankton
KW  - seawater
KW  - growth
KW  - temperature
Y1  - 2019
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-417126
SN  - 1866-8372
IS  - 667
ER  - 
TY  - JOUR
A1  - Masigol, Hossein
A1  - Khodaparast, Seyed Akbar
A1  - Mostowfizadeh-Ghalamfarsa, Reza
A1  - Rojas-Jimenez, Keilor
A1  - Woodhouse, Jason Nicholas
A1  - Neubauer, Darshan
A1  - Grossart, Hans-Peter
T1  - Taxonomical and functional diversity of Saprolegniales in Anzali lagoon, Iran
JF  - Aquatic Ecology
N2  - Studies on the diversity, distribution and ecological role of Saprolegniales (Oomycota) in freshwater ecosystems are currently receiving attention due to a greater understanding of their role in carbon cycling in various aquatic ecosystems. In this study, we characterized several Saprolegniales species isolated from Anzali lagoon, Gilan province, Iran, using morphological and molecular methods. Four species of Saprolegnia were identified, including S. anisospora and S. diclina as first reports for Iran, as well as Achlya strains, which were closely related to A. bisexualis, A. debaryana and A. intricata. Evaluation of the ligno-, cellulo- and chitinolytic activities was performed using plate assay methods. Most of the Saprolegniales isolates were obtained in autumn, and nearly 50% of the strains showed chitinolytic and cellulolytic activities. However, only a few Saprolegniales strains showed lignolytic activities. This study has important implications for better understanding the ecological niche of oomycetes, and to differentiate them from morphologically similar, but functionally different aquatic fungi in freshwater ecosystems.
KW  - Achlya
KW  - Saprolegnia
KW  - aquatic ecosystems
KW  - carbon cycling
KW  - polymer degradation
KW  - Saprolegniaceae
KW  - Achlyaceae
Y1  - 2020
U6  - https://doi.org/10.1007/s10452-019-09745-w
SN  - 1573-5125
SN  - 1386-2588
VL  - 54
IS  - 1
SP  - 323
EP  - 336
PB  - Springer Science
CY  - Dordrecht
ER  - 
TY  - JOUR
A1  - Xiao, Shangbin
A1  - Liu, Liu
A1  - Wang, Wei
A1  - Lorke, Andreas
A1  - Woodhouse, Jason Nicholas
A1  - Grossart, Hans-Peter
T1  - A Fast-Response Automated Gas Equilibrator (FaRAGE) for continuous in situ measurement of CH4 and CO2 dissolved in water
JF  - Hydrology and earth system sciences : HESS
N2  - Biogenic greenhouse gas emissions, e.g., of methane (CH4) and carbon dioxide (CO2) from inland waters, contribute substantially to global warming. In aquatic systems, dissolved greenhouse gases are highly heterogeneous in both space and time. To better understand the biological and physical processes that affect sources and sinks of both CH4 and CO2, their dissolved concentrations need to be measured with high spatial and temporal resolution. To achieve this goal, we developed the Fast-Response Automated Gas Equilibrator (FaRAGE) for real-time in situ measurement of dissolved CH4 and CO2 concentrations at the water surface and in the water column. FaRAGE can achieve an exceptionally short response time (t(95%) = 12 s when including the response time of the gas analyzer) while retaining an equilibration ratio of 62.6% and a measurement accuracy of 0.5% for CH4. A similar performance was observed for dissolved CO2 (t(95%) = 10 s, equilibration ratio 67.1 %). An equilibration ratio as high as 91.8% can be reached at the cost of a slightly increased response time (16 s). The FaRAGE is capable of continuously measuring dissolved CO2 and CH4 concentrations in the nM-to-submM (10(-9)-10(-3) mol L-1) range with a detection limit of subnM (10(-10) mol L-1), when coupling with a cavity ring-down greenhouse gas analyzer (Picarro GasScouter). FaRAGE allows for the possibility of mapping dissolved concentration in a "quasi" three-dimensional manner in lakes and provides an inexpensive alternative to other commercial gas equilibrators. It is simple to operate and suitable for continuous monitoring with a strong tolerance for suspended particles. While the FaRAGE is developed for inland waters, it can be also applied to ocean waters by tuning the gas-water mixing ratio. The FaRAGE is easily adapted to suit other gas analyzers expanding the range of potential applications, including nitrous oxide and isotopic composition of the gases.
Y1  - 2020
U6  - https://doi.org/10.5194/hess-24-3871-2020
SN  - 1027-5606
SN  - 1607-7938
VL  - 24
IS  - 7
SP  - 3871
EP  - 3880
PB  - European Geosciences Union (EGU) ; Copernicus
CY  - Munich
ER  - 
TY  - GEN
A1  - Ilicic, Doris
A1  - Woodhouse, Jason
A1  - Karsten, Ulf
A1  - Zimmermann, Jonas
A1  - Wichard, Thomas
A1  - Quartino, Maria Liliana
A1  - Campana, Gabriela Laura
A1  - Livenets, Alexandra
A1  - Van den Wyngaert, Silke
A1  - Grossart, Hans-Peter
T1  - Antarctic Glacial Meltwater Impacts the Diversity of Fungal Parasites Associated With Benthic Diatoms in Shallow Coastal Zones
T2  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - Aquatic ecosystems are frequently overlooked as fungal habitats, although there is increasing evidence that their diversity and ecological importance are greater than previously considered. Aquatic fungi are critical and abundant components of nutrient cycling and food web dynamics, e.g., exerting top-down control on phytoplankton communities and forming symbioses with many marine microorganisms. However, their relevance for microphytobenthic communities is almost unexplored. In the light of global warming, polar regions face extreme changes in abiotic factors with a severe impact on biodiversity and ecosystem functioning. Therefore, this study aimed to describe, for the first time, fungal diversity in Antarctic benthic habitats along the salinity gradient and to determine the co-occurrence of fungal parasites with their algal hosts, which were dominated by benthic diatoms. Our results reveal that Ascomycota and Chytridiomycota are the most abundant fungal taxa in these habitats. We show that also in Antarctic waters, salinity has a major impact on shaping not just fungal but rather the whole eukaryotic community composition, with a diversity of aquatic fungi increasing as salinity decreases. Moreover, we determined correlations between putative fungal parasites and potential benthic diatom hosts, highlighting the need for further systematic analysis of fungal diversity along with studies on taxonomy and ecological roles of Chytridiomycota.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1290 
KW  - Antarctica
KW  - aquatic fungi
KW  - Chytridiomycota
KW  - phytoplankton host
KW  - salinity gradient
KW  - Illumina amplicon sequencing
KW  - Carlini Station
Y1  - 2023
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-572895
SN  - 1866-8372
IS  - 1290
ER  - 
TY  - GEN
A1  - Masigol, Hossein
A1  - Khodaparast, Seyed Akbar
A1  - Mostowfizadeh-Ghalamfarsa, Reza
A1  - Rojas-Jimenez, Keilor
A1  - Woodhouse, Jason Nicholas
A1  - Neubauer, Darshan
A1  - Grossart, Hans-Peter
T1  - Taxonomical and functional diversity of Saprolegniales in Anzali lagoon, Iran
T2  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - Studies on the diversity, distribution and ecological role of Saprolegniales (Oomycota) in freshwater ecosystems are currently receiving attention due to a greater understanding of their role in carbon cycling in various aquatic ecosystems. In this study, we characterized several Saprolegniales species isolated from Anzali lagoon, Gilan province, Iran, using morphological and molecular methods. Four species of Saprolegnia were identified, including S. anisospora and S. diclina as first reports for Iran, as well as Achlya strains, which were closely related to A. bisexualis, A. debaryana and A. intricata. Evaluation of the ligno-, cellulo- and chitinolytic activities was performed using plate assay methods. Most of the Saprolegniales isolates were obtained in autumn, and nearly 50% of the strains showed chitinolytic and cellulolytic activities. However, only a few Saprolegniales strains showed lignolytic activities. This study has important implications for better understanding the ecological niche of oomycetes, and to differentiate them from morphologically similar, but functionally different aquatic fungi in freshwater ecosystems.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1395 
KW  - Achlya
KW  - Saprolegnia
KW  - aquatic ecosystems
KW  - carbon cycling
KW  - polymer degradation
KW  - Saprolegniaceae
KW  - Achlyaceae
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-515820
SN  - 1866-8372
IS  - 1
ER  - 
TY  - JOUR
A1  - Masigol, Hossein
A1  - Khodaparast, Seyed Akbar
A1  - Woodhouse, Jason Nicholas
A1  - Rojas Jiménez, Keilor
A1  - Fonvielle, Jeremy Andre
A1  - Rezakhani, Forough
A1  - Mostowfizadeh-Ghalamfarsa, Reza
A1  - Neubauer, Darshan
A1  - Goldhammer, Tobias
A1  - Grossart, Hans-Peter
T1  - The contrasting roles of aquatic fungi and oomycetes in the degradation and transformation of polymeric organic matter
JF  - Limnology and oceanography
N2  - Studies on the ecological role of fungi and, to a lesser extent, oomycetes, are receiving increasing attention, mainly due to their participation in the cycling of organic matter in aquatic ecosystems. To unravel their importance in humification processes, we isolated several strains of fungi and oomycetes from Anzali lagoon, Iran. We then performed taxonomic characterization by morphological and molecular methods, analyzed the ability to degrade several polymeric substrates, performed metabolic fingerprinting with Ecoplates, and determined the degradation of humic substances (HS) using liquid chromatography-organic carbon detection. Our analyses highlighted the capacity of aquatic fungi to better degrade a plethora of organic molecules, including complex polymers. Specifically, we were able to demonstrate not only the utilization of these complex polymers, but also the role of fungi in the production of HS. In contrast, oomycetes, despite some morphological and physiological similarities with aquatic fungi, exhibited a propensity toward opportunism, quickly benefitting from the availability of small organic molecules, while exhibiting sensitivity toward more complex polymers. Despite their contrasting roles, our study highlights the importance of both oomycetes and fungi in aquatic organic matter transformation and cycling with potential implications for the global carbon cycle.
Y1  - 2019
SN  - 0024-3590
SN  - 1939-5590
VL  - 64
IS  - 6
SP  - 2662
EP  - 2678
PB  - Wiley
CY  - Hoboken
ER  - 
TY  - JOUR
A1  - Hornick, Thomas
A1  - Bach, Lennart T.
A1  - Crawfurd, Katharine J.
A1  - Spilling, Kristian
A1  - Achterberg, Eric P.
A1  - Woodhouse, Jason Nicholas
A1  - Schulz, Kai G.
A1  - Brussaard, Corina P. D.
A1  - Riebesell, Ulf
A1  - Grossart, Hans-Peter
T1  - Ocean acidification impacts bacteria-phytoplankton coupling at low-nutrient conditions
JF  - Biogeosciences
N2  - The oceans absorb about a quarter of the annually produced anthropogenic atmospheric carbon dioxide (CO2), resulting in a decrease in surface water pH, a process termed ocean acidification (OA). Surprisingly little is known about how OA affects the physiology of heterotrophic bacteria or the coupling of heterotrophic bacteria to phytoplankton when nutrients are limited. Previous experiments were, for the most part, undertaken during productive phases or following nutrient additions designed to stimulate algal blooms. Therefore, we performed an in situ large-volume mesocosm (similar to 55 m(3)) experiment in the Baltic Sea by simulating different fugacities of CO2 (fCO(2)) extending from present to future conditions. The study was conducted in July-August after the nominal spring bloom, in order to maintain low-nutrient conditions throughout the experiment. This resulted in phytoplankton communities dominated by small-sized functional groups (picophytoplankton). There was no consistent fCO(2)-induced effect on bacterial protein production (BPP), cell-specific BPP (csBPP) or biovolumes (BVs) of either free-living (FL) or particle-associated (PA) heterotrophic bacteria, when considered as individual components (univariate analyses). Permutational Multivariate Analysis of Variance (PERMANOVA) revealed a significant effect of the fCO(2) treatment on entire assemblages of dissolved and particulate nutrients, metabolic parameters and the bacteria-phytoplankton community. However, distance-based linear modelling only identified fCO(2) as a factor explaining the variability observed amongst the microbial community composition, but not for explaining variability within the metabolic parameters. This suggests that fCO(2) impacts on microbial metabolic parameters occurred indirectly through varying physicochemical parameters and microbial species composition. Cluster analyses examining the co-occurrence of different functional groups of bacteria and phytoplankton further revealed a separation of the four fCO(2)-treated mesocosms from both control mesocosms, indicating that complex trophic interactions might be altered in a future acidified ocean. Possible consequences for nutrient cycling and carbon export are still largely unknown, in particular in a nutrient-limited ocean.
Y1  - 2017
U6  - https://doi.org/10.5194/bg-14-1-2017
SN  - 1726-4170
SN  - 1726-4189
VL  - 14
IS  - 1
SP  - 1
EP  - 15
PB  - Copernicus
CY  - Göttingen
ER  - 
TY  - JOUR
A1  - Hoke, Alexa
A1  - Woodhouse, Jason Nicholas
A1  - Zoccarato, Luca
A1  - McCarthy, Valerie
A1  - de Eyto, Elvira
A1  - Caldero-Pascual, Maria
A1  - Geffroy, Ewan
A1  - Dillane, Mary
A1  - Grossart, Hans-Peter
A1  - Jennings, Eleanor
T1  - Impacts of extreme weather events on bacterial community composition of a temperate humic lake
JF  - Water
N2  - Extreme weather events are projected to increase in frequency and intensity as climate change continues. Heterotrophic bacteria play a critical role in lake ecosystems, yet little research has been done to determine how they are affected by such extremes. The purpose of this study was to use high-throughput sequencing to explore the bacterial community composition of a humic oligotrophic lake on the North Atlantic Irish coast and to assess the impacts on composition dynamics related to extreme weather events. Samples for sequencing were collected from Lough Feeagh on a fortnightly basis from April to November 2018. Filtration was used to separate free-living and particle-associated bacterial communities and amplicon sequencing was performed for the 16S rRNA V4 region. Two named storms, six high discharge events, and one drought period occurred during the sampling period. These events had variable, context-dependent effects on bacterial communities in Lough Feeagh. The particle-associated community was found to be more likely to respond to physical changes, such as mixing, while the free-living population responded to changes in nutrient and carbon concentrations. Generally, however, the high stability of the bacterial community observed in Lough Feeagh suggests that the bacterial community is relatively resilient to extreme weather events.
KW  - extreme weather event
KW  - storm
KW  - drought
KW  - bacteria
KW  - free-living
KW  - particle-associated
KW  - humic lake
Y1  - 2020
U6  - https://doi.org/10.3390/w12102757
SN  - 2073-4441
VL  - 12
IS  - 10
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Batista, A. M. M.
A1  - Woodhouse, Jason Nicholas
A1  - Grossart, Hans-Peter
A1  - Giani, A.
T1  - Methanogenic archaea associated to Microcystis sp. in field samples and in culture
JF  - Hydrobiologia : acta hydrobiologica, hydrographica, limnologica et protistologica
N2  - Cyanobacterial mass developments impact the community composition of heterotrophic microorganisms with far-reaching consequences for biogeochemical and energy cycles of freshwater ecosystems including reservoirs. Here we sought to evaluate the temporal stability of methanogenic archaea in the water column and further scrutinize their associations with cyanobacteria. Monthly samples were collected from October 2009 to December 2010 in hypereutrophic Pampulha reservoir with permanently blooming cyanobacteria, and from January to December 2011 in oligotrophic Volta Grande reservoir with only sporadic cyanobacteria incidence. The presence of archaea in cyanobacterial cultures was investigated by screening numerous strains of Microcystis spp. from these reservoirs as well as from lakes in Europe, Asia, and North-America. We consistently determined the occurrence of archaea, in particular methanogenic archaea, in both reservoirs throughout the year. However, archaea were only associated with two strains (Microcystis sp. UFMG 165 and UFMG 175) recently isolated from these reservoirs. These findings do not implicate archaea in the occurrence of methane in the epilimnion of inland waters, but rather serve to highlight the potential of microhabitats associated with particles, including phytoplankton, to shelter unique microbial communities.
KW  - Cyanobacteria
KW  - Methanogenic archaea
KW  - Bacterial community composition
KW  - Microcystis sp
KW  - Tropical reservoir
Y1  - 2018
U6  - https://doi.org/10.1007/s10750-018-3655-3
SN  - 0018-8158
SN  - 1573-5117
VL  - 831
IS  - 1
SP  - 163
EP  - 172
PB  - Springer
CY  - Dordrecht
ER  -