TY  - JOUR
A1  - Folkesson, Maggie
A1  - Vorkapic, Emina
A1  - Gulbins, Erich
A1  - Japtok, Lukasz
A1  - Kleuser, Burkhard
A1  - Welander, Martin
A1  - Länne, Toste
A1  - Wågsäter, Dick
T1  - Inflammatory cells, ceramides, and expression of proteases in perivascular adipose tissue adjacent to human abdominal aortic aneurysms
JF  - Journal of vascular surgery
N2  - Background: Abdominal aortic aneurysm (AAA) is a deadly irreversible weakening and distension of the abdominal aortic wall. The pathogenesis of AAA remains poorly understood. Investigation into the physical and molecular characteristics of perivascular adipose tissue (PVAT) adjacent to AAA has not been done before and is the purpose of this study. 

Methods and Results: Human aortae, periaortic PVAT, and fat surrounding peripheral arteries were collected from patients undergoing elective surgical repair of AAA. Control aortas were obtained from recently deceased healthy organ donors with no known arterial disease. Aorta and PVAT was found in AAA to larger extent compared with control aortas. Immunohistochemistry revealed neutrophils, macrophages, mast cells, and T-cells surrounding necrotic adipocytes. Gene expression analysis showed that neutrophils, mast cells, and T-cells were found to be increased in PVAT compared with AAA as well as cathepsin K and S. The concentration of ceramides in PVAT was determined using mass spectrometry and correlated with content of T-cells in the PVAT. 

Conclusions: Our results suggest a role for abnormal necrotic, inflamed, proteolytic adipose tissue to the adjacent aneurysmal aortic wall in ongoing vascular damage.
Y1  - 2016
U6  - https://doi.org/10.1016/j.jvs.2015.12.056
SN  - 0741-5214
VL  - 65
IS  - 4
SP  - 1171
EP  - 1179
PB  - Elsevier
CY  - New York
ER  -