TY - GEN A1 - Olmer, Ruth A1 - Engels, Lena A1 - Usman, Abdulai A1 - Menke, Sandra A1 - Malik, Muhammad Nasir Hayat A1 - Pessler, Frank A1 - Göhring, Gudrun A1 - Bornhorst, Dorothee A1 - Bolten, Svenja A1 - Abdelilah-Seyfried, Salim A1 - Scheper, Thomas A1 - Kempf, Henning A1 - Zweigerdt, Robert A1 - Martin, Ulrich T1 - Differentiation of Human Pluripotent Stem Cells into Functional Endothelial Cells in Scalable Suspension Culture T2 - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe N2 - Endothelial cells (ECs) are involved in a variety of cellular responses. As multifunctional components of vascular structures, endothelial (progenitor) cells have been utilized in cellular therapies and are required as an important cellular component of engineered tissue constructs and in vitro disease models. Although primary ECs from different sources are readily isolated and expanded, cell quantity and quality in terms of functionality and karyotype stability is limited. ECs derived from human induced pluripotent stem cells (hiPSCs) represent an alternative and potentially superior cell source, but traditional culture approaches and 2D differentiation protocols hardly allow for production of large cell numbers. Aiming at the production of ECs, we have developed a robust approach for efficient endothelial differentiation of hiPSCs in scalable suspension culture. The established protocol results in relevant numbers of ECs for regenerative approaches and industrial applications that show in vitro proliferation capacity and a high degree of chromosomal stability. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1182 KW - virus infection KW - progenitor cells KW - in vitro KW - telomere dysfunction KW - cord blood KW - cardiomyogenic differentiation KW - angiogenesis KW - efficient KW - aberrations KW - expression Y1 - 2017 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-427095 SN - 1866-8372 IS - 5 ER - TY - JOUR A1 - Olmer, Ruth A1 - Engels, Lena A1 - Usman, Abdulai A1 - Menke, Sandra A1 - Malik, Muhammad Nasir Hayat A1 - Pessler, Frank A1 - Goehring, Gudrun A1 - Bornhorst, Dorothee A1 - Bolten, Svenja A1 - Abdelilah-Seyfried, Salim A1 - Scheper, Thomas A1 - Kempf, Henning A1 - Zweigerdt, Robert A1 - Martin, Ulrich T1 - Differentiation of Human Pluripotent Stem Cells into Functional Endothelial Cells in Scalable Suspension Culture JF - Stem Cell Reports N2 - Endothelial cells (ECs) are involved in a variety of cellular responses. As multifunctional components of vascular structures, endothelial (progenitor) cells have been utilized in cellular therapies and are required as an important cellular component of engineered tissue constructs and in vitro disease models. Although primary ECs from different sources are readily isolated and expanded, cell quantity and quality in terms of functionality and karyotype stability is limited. ECs derived from human induced pluripotent stem cells (hiPSCs) represent an alternative and potentially superior cell source, but traditional culture approaches and 2D differentiation protocols hardly allow for production of large cell numbers. Aiming at the production of ECs, we have developed a robust approach for efficient endothelial differentiation of hiPSCs in scalable suspension culture. The established protocol results in relevant numbers of ECs for regenerative approaches and industrial applications that show in vitro proliferation capacity and a high degree of chromosomal stability. KW - virus infection KW - progenitor cells KW - in vitro KW - telomere dysfunction KW - cord blood KW - cardiomyogenic differentiation KW - angiogenesis KW - efficient KW - aberrations KW - expression Y1 - 2017 U6 - https://doi.org/10.1016/j.stemcr.2018.03.017 SN - 2213-6711 VL - 10 IS - 5 PB - Springer CY - New York ER - TY - CHAP A1 - Motaln, Helena A1 - Schuchhardt, Johannes A1 - Stec, Karol A1 - Breznik, Barbara A1 - Ulrich, Henning A1 - Lah, Tamara T. T1 - Paradoxical role of mesenchymal stem cells in the glioblastoma microenvironment T2 - Anticancer research : international journal of cancer research and treatment Y1 - 2014 SN - 0250-7005 SN - 1791-7530 VL - 34 IS - 10 SP - 6071 EP - 6072 PB - International Institute of Anticancer Research CY - Athens ER - TY - JOUR A1 - Markschies, Christoph A1 - Päßler, Ulrich A1 - Grote, Mathias A1 - Greenwood MacKinney, Anne A1 - Kusber, Wolf-Henning A1 - Jahn, Regine A1 - Damaschun, Ferdinand A1 - Böhme, Katrin ED - Ette, Ottmar ED - Knobloch, Eberhard ED - Päßler, Ulrich T1 - HiN : Alexander von Humboldt im Netz = Christian Gottfried Ehrenberg BT - Lebensbilder eines Naturforschers N2 - -Christoph Markschies: Geleitwort -Ulrich Päßler: Christian Gottfried Ehrenberg: Lebensbilder eines Naturforschers -Mathias Grote: „Aus dem Kleinen bauen sich die Welten“ – Christian Gottfried Ehrenbergs ökologische Mikrobiologie avant la lettre -Anne Greenwood MacKinney: Die Inszenierung naturforschender Gelehrsamkeit beim Sammeln: Christian Gottfried Ehrenbergs und Wilhelm Hemprichs nordafrikanische Forschungsreise (1820 – 1825) -Ulrich Päßler: Reisen im Nahen Osten. Zeichnungen -Ulrich Päßler: Christian Gottfried Ehrenberg und die Biogeographie: Die russisch-sibirische Reise mit Alexander von Humboldt (1829) -Ulrich Päßler: Russisch-Sibirische Reise. Zeichnungen -Wolf-Henning Kusber, Regine Jahn: Christian Gottfried Ehrenbergs Zeichnungen: Eine frühe wissenschaftliche Dokumentation mikroskopischer Organismen -Ferdinand Damaschun: Christian Gottfried Ehrenberg und die Entwicklung der Mikroskop-Technik im 19. Jahrhundert -Ulrich Päßler: Die Reise ins Kleinste der Natur. Zeichnungen -Katrin Böhme: Das große Ganze: Christian Gottfried Ehrenberg und die Gesellschaft Naturforschender Freunde zu Berlin T3 - HiN : Alexander von Humboldt im Netz ; International Review for Humboldtian Studies - XXII. 2021, 42 KW - Christian Gottfried Ehrenberg KW - Naturgemälde KW - Mikrobiologie KW - Infusionsthierchen KW - Alexander von Humboldt KW - Wissenschaftsgeschichte KW - Forschungsreisen KW - Mikroskopie KW - Ehrenberg KW - Ökologie KW - Cholera KW - Protistologie KW - Infektion KW - Infusorium KW - Wilhelm Hemprich KW - gelehrte Tugenden KW - Sammlungspraxis KW - Dokumentationspraxis KW - wissenschaftliche Zeichnungen KW - Biogeographie KW - Algen KW - Artbeschreibung KW - Biodiversität KW - naturkundliche Sammlung KW - Glas KW - Infusorien KW - Mikrogeologie KW - Chevalier KW - Pistor & Schiek KW - Geschichte der Mikroskopie KW - wissenschaftliche Instrumente KW - Gesellschaft Naturforschender Freunde zu Berlin KW - GNF KW - Infusorienwerke Y1 - 2021 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-501413 SN - 2568-3543 SN - 1617-5239 VL - XXII IS - 42 PB - Universitätsverlag Potsdam CY - Potsdam ER - TY - JOUR A1 - Wittig, Alice A1 - Miranda, Fabio Malcher A1 - Hölzer, Martin A1 - Altenburg, Tom A1 - Bartoszewicz, Jakub Maciej A1 - Beyvers, Sebastian A1 - Dieckmann, Marius Alfred A1 - Genske, Ulrich A1 - Giese, Sven Hans-Joachim A1 - Nowicka, Melania A1 - Richard, Hugues A1 - Schiebenhoefer, Henning A1 - Schmachtenberg, Anna-Juliane A1 - Sieben, Paul A1 - Tang, Ming A1 - Tembrockhaus, Julius A1 - Renard, Bernhard Y. A1 - Fuchs, Stephan T1 - CovRadar BT - continuously tracking and filtering SARS-CoV-2 mutations for genomic surveillance JF - Bioinformatics N2 - The ongoing pandemic caused by SARS-CoV-2 emphasizes the importance of genomic surveillance to understand the evolution of the virus, to monitor the viral population, and plan epidemiological responses. Detailed analysis, easy visualization and intuitive filtering of the latest viral sequences are powerful for this purpose. We present CovRadar, a tool for genomic surveillance of the SARS-CoV-2 Spike protein. CovRadar consists of an analytical pipeline and a web application that enable the analysis and visualization of hundreds of thousand sequences. First, CovRadar extracts the regions of interest using local alignment, then builds a multiple sequence alignment, infers variants and consensus and finally presents the results in an interactive app, making accessing and reporting simple, flexible and fast. Y1 - 2022 U6 - https://doi.org/10.1093/bioinformatics/btac411 SN - 1367-4803 SN - 1367-4811 VL - 38 IS - 17 SP - 4223 EP - 4225 PB - Oxford Univ. Press CY - Oxford ER -