TY - JOUR A1 - Jonscher, Ernst A1 - Flemming, Sven A1 - Schmitt, Marius A1 - Sabitzki, Ricarda A1 - Reichard, Nick A1 - Birnbaum, Jakob A1 - Bergmann, Bärbel A1 - Höhn, Katharina A1 - Spielmann, Tobias T1 - PfVPS45 Is Required for Host Cell Cytosol Uptake by Malaria Blood Stage Parasites JF - Cell host & microbe N2 - During development in human erythrocytes, the malaria parasite Plasmodium falciparum internalizes a large part of the cellular content of the host cell. The internalized cytosol, consisting largely of hemoglobin, is transported to the parasite’s food vacuole where it is degraded, providing nutrients and space for growth. This host cell cytosol uptake (HCCU) is crucial for parasite survival but the parasite proteins mediating this process remain obscure. Here, we identify P. falciparum VPS45 as an essential factor in HCCU. Conditional inactivation of PfVPS45 led to an accumulation of host cell cytosol-filled vesicles within the parasite and inhibited the delivery of hemoglobin to the parasite's digestive vacuole, resulting in arrested parasite growth. A proportion of these HCCU vesicle intermediates was positive for phosphatidylinositol 3-phosphate, suggesting endosomal characteristics. Thus PfVPS45 provides insight into the elusive machinery of the ingestion pathway in a parasite that contains an endolysosomal system heavily repurposed for protein secretion. Y1 - 2018 U6 - https://doi.org/10.1016/j.chom.2018.11.010 SN - 1931-3128 SN - 1934-6069 VL - 25 IS - 1 SP - 166 EP - 173 PB - Cell Press CY - Cambridge ER - TY - JOUR A1 - Hagen, Sven A1 - Baumann, Tobias A1 - Wagner, Hanna J. A1 - Morath, Volker A1 - Kaufmann, Beate A1 - Fischer, Adrian A1 - Bergmann, Stefan A1 - Schindler, Patrick A1 - Arndt, Katja Maren A1 - Mueller, Kristian M. T1 - Modular adeno-associated virus (rAAV) vectors used for cellular virus-directed enzyme prodrug therapy JF - Scientific reports N2 - The pre-clinical and clinical development of viral vehicles for gene transfer increased in recent years, and a recombinant adeno-associated virus (rAAV) drug took center stage upon approval in the European Union. However, lack of standardization, inefficient purification methods and complicated retargeting limit general usability. We address these obstacles by fusing rAAV-2 capsids with two modular targeting molecules (DARPin or Affibody) specific for a cancer cell-surface marker (EGFR) while simultaneously including an affinity tag (His-tag) in a surface-exposed loop. Equipping these particles with genes coding for prodrug converting enzymes (thymidine kinase or cytosine deaminase) we demonstrate tumor marker specific transduction and prodrug-dependent apoptosis of cancer cells. Coding terminal and loop modifications in one gene enabled specific and scalable purification. Our genetic parts for viral production adhere to a standardized cloning strategy facilitating rapid prototyping of virus directed enzyme prodrug therapy (VDEPT). Y1 - 2014 U6 - https://doi.org/10.1038/srep03759 SN - 2045-2322 VL - 4 PB - Nature Publ. Group CY - London ER - TY - JOUR A1 - Dey, Pradip A1 - Bergmann, Tobias A1 - Cuellar-Camacho, Jose Luis A1 - Ehrmann, Svenja A1 - Chowdhury, Mohammad Suman A1 - Zhang, Minze A1 - Dahmani, Ismail A1 - Haag, Rainer A1 - Azad, Walid T1 - Multivalent flexible nanogels exhibit broad-spectrum antiviral activity by blocking virus entry JF - ACS nano N2 - The entry process of viruses into host cells is complex and involves stable but transient multivalent interactions with different cell surface receptors. The initial contact of several viruses begins with attachment to heparan sulfate (HS) proteoglycans on the cell surface, which results in a cascade of events that end up with virus entry. The development of antiviral agents based on multivalent interactions to shield virus particles and block initial interactions with cellular receptors has attracted attention in antiviral research. Here, we designed nanogels with different degrees of flexibility based on dendritic polyglycerol sulfate to mimic cellular HS. The designed nanogels are nontoxic and broad-spectrum, can multivalently interact with viral glycoproteins, shield virus surfaces, and efficiently block infection. We also visualized virus-nanogel interactions as well as the uptake of nanogels by the cells through clathrin-mediated endocytosis using confocal microscopy. As many human viruses attach to the cells through HS moieties, we introduce our flexible nanogels as robust inhibitors for these viruses. KW - multivalent KW - herpes simplex virus KW - heparan sulfate KW - nanoparticles KW - click chemistry KW - polyglycerol Y1 - 2018 U6 - https://doi.org/10.1021/acsnano.8b01616 SN - 1936-0851 SN - 1936-086X VL - 12 IS - 7 SP - 6429 EP - 6442 PB - American Chemical Society CY - Washington ER - TY - JOUR A1 - Amberg, Maximilian A1 - aus dem Moore, Nils A1 - Bekk, Anke A1 - Bergmann, Tobias A1 - Edenhofer, Ottmar A1 - Flachsland, Christian A1 - George, Jan A1 - Haywood, Luke A1 - Heinemann, Maik A1 - Held, Anne A1 - Kalkuhl, Matthias A1 - Kellner, Maximilian A1 - Koch, Nicolas A1 - Luderer, Gunnar A1 - Meyer, Henrika A1 - Nikodinoska, Dragana A1 - Pahle, Michael A1 - Roolfs, Christina A1 - Schill, Wolf-Peter T1 - Reformoptionen für ein nachhaltiges Steuer- und Abgabensystem BT - wie Lenkungssteuern effektiv und gerecht für den Klima- und Umweltschutz ausgestaltet werden können JF - Perspektiven der Wirtschaftspolitik N2 - Steuern und Abgaben auf Produkte oder Verbrauch mit gesellschaftlichen Folgekosten (externe Kosten) – sogenannte Pigou- oder Lenkungssteuern – sind ein gesellschaftliches „Win-Win-Instrument“. Sie verbessern die Wohlfahrt und schützen gleichzeitig die Umwelt und das Klima. Dies wird erreicht, indem umweltschädigende Aktivitäten einen Preis bekommen, der möglichst exakt der Höhe des Schadens entspricht. Eine konsequente Bepreisung der externen Kosten nach diesem Prinzip könnte in Deutschland erhebliche zusätzliche Einnahmen erbringen: Basierend auf bisherigen Studien zu externen Kosten wären zusätzliche Einnahmen in der Größenordnung von 348 bis 564 Milliarden Euro pro Jahr (44 bis 71 Prozent der gesamten Steuereinnahmen) möglich. Die Autoren warnen allerdings, dass die Bezifferung der externen Kosten mit erheblichen Unsicherheiten verbunden ist. Damit Lenkungssteuern und -abgaben ihre positiven Lenkungs- und Wohlstandseffekte voll entfalten können, seien zudem institutionelle Reformen notwendig. KW - Externalitäten KW - Pigou-Steuern KW - Nachhaltige Steuerreform KW - Energiewende Y1 - 2022 U6 - https://doi.org/10.1515/pwp-2021-0051 SN - 1465-6493 SN - 1468-2516 VL - 23 IS - 3 SP - 165 EP - 199 PB - De Gruyter CY - Berlin ER - TY - RPRT A1 - Kalkuhl, Matthias A1 - Flachsland, Christian A1 - Knopf, Brigitte A1 - Amberg, Maximilian A1 - Bergmann, Tobias A1 - Kellner, Maximilian A1 - Stüber, Sophia A1 - Haywood, Luke A1 - Roolfs, Christina A1 - Edenhofer, Ottmar T1 - Effects of the energy price crisis on households in Germany BT - socio-political challenges and policy options Y1 - 2022 UR - https://www.mcc-berlin.net/fileadmin/data/C18_MCC_Publications/2022_MCC_Effects_of_the_energy_price_crisis_on_households.pdf PB - Mercator Research Institute on Global Commons and Climate Change (MCC) gGmbH CY - Berlin ER - TY - RPRT A1 - Kalkuhl, Matthias A1 - Flachsland, Christian A1 - Knopf, Brigitte A1 - Amberg, Maximilian A1 - Bergmann, Tobias A1 - Kellner, Maximilian A1 - Stüber, Sophia A1 - Haywood, Luke A1 - Roolfs, Christina A1 - Edenhofer, Ottmar T1 - Auswirkungen der Energiepreiskrise auf Haushalte in Deutschland BT - sozialpolitische Herausforderungen und Handlungsoptionen Y1 - 2022 UR - https://www.mcc-berlin.net/fileadmin/data/C18_MCC_Publications/2022_MCC_Auswirkungen_der_Energiepreiskrise_auf_Haushalte.pdf PB - Mercator Research Institute on Global Commons and Climate Change (MCC) gGmbH CY - Berlin ER - TY - RPRT A1 - Kalkuhl, Matthias A1 - Amberg, Maximilian A1 - Bergmann, Tobias A1 - Knopf, Brigitte A1 - Edenhofer, Ottmar T1 - Gaspreisdeckel, Mehrwertsteuersenkung, Energiepauschale BT - wie kann die Bevölkerung zielgenau und schnell entlastet werden? Y1 - 2022 UR - https://www.mcc-berlin.net/fileadmin/data/C18_MCC_Publications/2022_MCC_Gaspreise_und_Entlastungsma%C3%9Fnahmen.pdf PB - Mercator Research Institute on Global Commons and Climate Change (MCC) gGmbH CY - Berlin ER - TY - RPRT A1 - Kellner, Maximilian A1 - Amberg, Maximilian A1 - Bergmann, Tobias A1 - Roolfs, Christina A1 - Kalkuhl, Matthias T1 - Entlastungspakete für Energiepreisanstiege BT - Auswirkungen und Nachbesserungsbedarf Y1 - 2022 UR - https://www.mcc-berlin.net/fileadmin/user_upload/Kalkuhl/2022_MCC_Entlastungspakete_fuer_Energiepreisanstiege_.pdf U6 - https://doi.org/10.5281/zenodo.6617130 PB - Mercator Research Institute on Global Commons and Climate Change (MCC) gGmbH CY - Berlin ER - TY - JOUR A1 - Schwingshackl, Lukas A1 - Ruzanska, Ulrike Alexandra A1 - Anton, Verena A1 - Wallroth, Raphael A1 - Ohla, Kathrin A1 - Knueppel, Sven A1 - Schulze, Matthias Bernd A1 - Pischon, Tobias A1 - Deutschbein, Johannes A1 - Schenk, Liane A1 - Warschburger, Petra A1 - Harttig, Ulrich A1 - Boeing, Heiner A1 - Bergmann, Manuela M. T1 - The NutriAct Family Study: a web-based prospective study on the epidemiological, psychological and sociological basis of food choice JF - BMC public health N2 - Background: Most studies on food choice have been focussing on the individual level but familial aspects may also play an important role. This paper reports of a novel study that will focus on the familial aspects of the formation of food choice among men and women aged 50-70 years by recruiting spouses and siblings (NutriAct Family Study; NFS). Discussion: Until August 4th 2017, 4783 EPIC-Participants were contacted by mail of which 446 persons recruited 2 to 5 family members (including themselves) resulting in 1032 participants, of whom 82% had started answering or already completed the questionnaires. Of the 4337 remaining EPIC-participants who had been contacted, 1040 (24%) did not respond at all, and 3297 (76%) responded but declined, in 51% of the cases because of the request to recruit at least 2 family members in the respective age range. The developed recruitment procedures and web-based methods of data collection are capable to generate the required study population including the data on individual and inter-personal determinants which will be linkable to food choice. The information on familial links among the study participants will show the role of familial traits in midlife for the adoption of food choices supporting healthy aging. KW - NutriAct family study KW - Study protocol KW - Food choice KW - Determinants Y1 - 2018 U6 - https://doi.org/10.1186/s12889-018-5814-x SN - 1471-2458 VL - 18 PB - BMC CY - London ER -