TY - JOUR A1 - Ebert, Franziska A1 - Thomann, Marlies A1 - Witt, Barbara A1 - Müller, Sandra Marie A1 - Meyer, Sören A1 - Weber, Till A1 - Christmann, Markus A1 - Schwerdtle, Tanja T1 - Evaluating long-term cellular effects of the arsenic species thio-DMA(V): qPCR-based gene expression as screening tool JF - Journal of trace elements in medicine and biology N2 - Thio-dimethylarsinic acid (thio-DMA(V)) is a human urinary metabolite of the class 1 human carcinogen inorganic arsenic as well as of arsenosugars. Thio-DMA(V) exerts strong cellular toxicity, whereas its toxic modes of action are not fully understood. For the first time, this study characterises the impact of a long-term (21 days) in vitro incubation of thio-DMA(V) on the expression of selected genes related to cell death, stress response, epigenetics and DNA repair. The observed upregulation of DNMT1 might be a cellular compensation to counterregulate the in a very recent study observed massive global DNA hypomethylation after chronic thio-DMAv incubation. Moreover, our data suggest that chronic exposure towards subcytotoxic, pico- to nanomolar concentrations of thio-DMA(V) causes a stress response in human urothelial cells. The upregulation of genes encoding for proteins of DNA repair (Apex1,Lig1, XRCC1,DDB2, XPG, ATR) as well as damage response (GADD45A, GADD45G, Trp53) indicate a potential genotoxic risk emanating from thio-DMA(V) after long-term incubation. (C) 2016 Elsevier GmbH. All rights reserved. KW - Thio-dimethylarsinic acid KW - Long-term cellular toxicity KW - qPCR-based gene expression screening KW - GADD45A and GADD45G KW - DNMT1 KW - Cellular damage response Y1 - 2016 U6 - https://doi.org/10.1016/j.jtemb.2016.06.004 SN - 0946-672X VL - 37 SP - 78 EP - 84 PB - Yokohama Publishers CY - Jena ER - TY - THES A1 - Weber, Florian Till T1 - In vitro Toxizität von Elektrolytlösungen der Lithium-Ionen Batterie Y1 - 2016 ER - TY - BOOK A1 - Mientus, Lukas A1 - Klempin, Christiane A1 - Nowak, Anna A1 - Wyss, Corinne A1 - Aufschnaiter, Claudia von A1 - Faix, Ann-Christin A1 - te Poel, Kathrin A1 - Wahbe, Nadia A1 - Pieper, Martin A1 - Höller, Katharina A1 - Kallenbach, Lea A1 - Förster, Magdalena A1 - Redecker, Anke A1 - Dick, Mirjam A1 - Holle, Jörg A1 - Schneider, Edina A1 - Rehfeldt, Daniel A1 - Brauns, Sarah A1 - Abels, Simone A1 - Ferencik-Lehmkuhl, Daria A1 - Fränkel, Silvia A1 - Frohn, Julia A1 - Liebsch, Ann-Catherine A1 - Pech, Detlef A1 - Schreier, Pascal A1 - Jessen, Moiken A1 - Großmann, Uta A1 - Skintey, Lesya A1 - Voerkel, Paul A1 - Vaz Ferreira, Mergenfel A. A1 - Zimmermann, Jan-Simon A1 - Buddeberg, Magdalena A1 - Henke, Vanessa A1 - Hornberg, Sabine A1 - Völschow, Yvette A1 - Warrelmann, Julia-Nadine A1 - Malek, Jennifer A1 - Tinnefeld, Anja A1 - Schmidt, Peggy A1 - Bauer, Tobias A1 - Jänisch, Christopher A1 - Spitzer, Lisa A1 - Franken, Nadine A1 - Degeling, Maria A1 - Preisfeld, Angelika A1 - Meier, Jana A1 - Küth, Simon A1 - Scholl, Daniel A1 - Vogelsang, Christoph A1 - Watson, Christina A1 - Weißbach, Anna A1 - Kulgemeyer, Christoph A1 - Oetken, Mandy A1 - Gorski, Sebastian A1 - Kubsch, Marcus A1 - Sorge, Stefan A1 - Wulff, Peter A1 - Fellenz, Carolin D. A1 - Schnell, Susanne A1 - Larisch, Cathleen A1 - Kaiser, Franz A1 - Knott, Christina A1 - Reimer, Stefanie A1 - Stegmüller, Nathalie A1 - Boukrayâa Trabelsi, Kathrin A1 - Schißlbauer, Franziska A1 - Lemberger, Lukas A1 - Barth, Ulrike A1 - Wiehl, Angelika A1 - Rogge, Tim A1 - Böhnke, Anja A1 - Dietz, Dennis A1 - Großmann, Leroy A1 - Wienmeister, Annett A1 - Zoppke, Till A1 - Jiang, Lisa A1 - Grünbauer, Stephanie A1 - Ostersehlt, Dörte A1 - Peukert, Sophia A1 - Schäfer, Christoph A1 - Löbig, Anna A1 - Bröll, Leena A1 - Brandt, Birgit A1 - Breuer, Meike A1 - Dausend, Henriette A1 - Krelle, Michael A1 - Andersen, Gesine A1 - Falke, Sascha A1 - Kindermann-Güzel, Kristin A1 - Körner, Katrina A1 - Lottermoser, Lisa-Marie A1 - Pügner, Kati A1 - Sonnenburg, Nadine A1 - Akarsu, Selim A1 - Rechl, Friederike A1 - Gadinger, Laureen A1 - Heinze, Lena A1 - Wittmann, Eveline A1 - Franke, Manuela A1 - Lachmund, Anne-Marie A1 - Böttger, Julia A1 - Hannover, Bettina A1 - Behrendt, Renata A1 - Conty, Valentina A1 - Grundmann, Stephanie A1 - Ghassemi, Novid A1 - Opitz, Ben A1 - Brämer, Martin A1 - Gasparjan, David A1 - Sambanis, Michaela A1 - Köster, Hilde A1 - Lücke, Martin A1 - Nordmeier, Volkhard A1 - Schaal, Sonja A1 - Haberbosch, Maximilian A1 - Meissner, Maren A1 - Schaal, Steffen A1 - Brüchner, Melanie A1 - Riehle, Tamara A1 - Leopold, Bengta Marie A1 - Gerlach, Susanne A1 - Rau-Patschke, Sarah A1 - Skorsetz, Nina A1 - Weber, Nadine A1 - Damköhler, Jens A1 - Elsholz, Markus A1 - Trefzger, Thomas A1 - Lewek, Tobias A1 - Borowski, Andreas ED - Mientus, Lukas ED - Klempin, Christiane ED - Nowak, Anna T1 - Reflexion in der Lehrkräftebildung BT - Empirisch – Phasenübergreifend – Interdisziplinär T3 - Potsdamer Beiträge für Lehrkräftebildung und Bildungsforschung N2 - Reflexion ist eine Schlüsselkategorie für die professionelle Entwicklung von Lehrkräften, welche als Ausbildungsziel in den Bildungsstandards für die Lehrkräftebildung verankert ist. Eine Verstetigung universitär geprägter Forschung und Modellierung in der praxisnahen Anwendung im schulischen Kontext bietet Potentiale nachhaltiger Professionalisierung. Die Stärkung reflexionsbezogener Kompetenzen durch Empirie und Anwendung scheint eine phasenübergreifende Herausforderung der Lehrkräftebildung zu sein, die es zu bewältigen gilt. Ziele des Tagungsbandes Reflexion in der Lehrkräftebildung sind eine theoretische Schärfung des Konzeptes „Reflexive Professionalisierung“ und der Austausch über Fragen der Einbettung wirksamer reflexionsbezogener Lerngelegenheiten in die Lehrkräftebildung. Forschende und Lehrende der‚ drei Phasen (Studium, Referendariat sowie Fort- und Weiterbildung) der Lehrkräftebildung stellen Lehrkonzepte und Forschungsprojekte zum Thema Reflexion in der Lehrkräftebildung vor und diskutieren diese. Gemeinsam mit Teilnehmenden aller Phasen und von verschiedenen Standorten der Lehrkräftebildung werden zukünftige Herausforderungen identifiziert und Lösungsansätze herausgearbeitet. T3 - Potsdamer Beiträge zur Lehrkräftebildung und Bildungsforschung - 4 KW - Reflexion KW - Lehrkräftebildung KW - Reflexionskompetenz KW - Reflexivität KW - Feedback KW - Reflection KW - Teacher Education KW - Reflection Skills KW - Reflexivity KW - Feedback Y1 - 2023 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-591717 SN - 978-3-86956-566-8 SN - 2626-3556 SN - 2626-4722 IS - 4 PB - Universitätsverlag Potsdam CY - Potsdam ER - TY - JOUR A1 - Pieper, Imke A1 - Wehe, Christoph A. A1 - Bornhorst, Julia A1 - Ebert, Franziska A1 - Leffers, Larissa A1 - Holtkamp, Michael A1 - Höseler, Pia A1 - Weber, Till A1 - Mangerich, Aswin A1 - Bürkle, Alexander A1 - Karst, Uwe A1 - Schwerdtle, Tanja T1 - Mechanisms of Hg species induced toxicity in cultured human astrocytes BT - genotoxicity and DNA-damage response JF - Metallomics N2 - The toxicologically most relevant mercury (Hg) species for human exposure is methylmercury (MeHg). Thiomersal is a common preservative used in some vaccine formulations. The aim of this study is to get further mechanistic insight into the yet not fully understood neurotoxic modes of action of organic Hg species. Mercury species investigated include MeHgCl and thiomersal. Additionally HgCl2 was studied, since in the brain mercuric Hg can be formed by dealkylation of the organic species. As a cellular system astrocytes were used. In vivo astrocytes provide the environment necessary for neuronal function. In the present study, cytotoxic effects of the respective mercuricals increased with rising alkylation level and correlated with their cellular bioavailability. Further experiments revealed for all species at subcytotoxic concentrations no induction of DNA strand breaks, whereas all species massively increased H2O2-induced DNA strand breaks. This co-genotoxic effect is likely due to a disturbance of the cellular DNA damage response. Thus, at nanomolar, sub-cytotoxic concentrations, all three mercury species strongly disturbed poly(ADP-ribosyl)ation, a signalling reaction induced by DNA strand breaks. Interestingly, the molecular mechanism behind this inhibition seems to be different for the species. Since chronic PARP-1 inhibition is also discussed to sacrifice neurogenesis and learning abilities, further experiments on neurons and in vivo studies could be helpful to clarify whether the inhibition of poly(ADP-ribosyl)ation contributes to organic Hg induced neurotoxicity. KW - cell-death KW - poly(ADP-ribose) polymerase-1 KW - neurodegenerative diseases KW - adduct formation KW - thimerosal KW - methylmercury KW - repair KW - neurotoxicity KW - manganese KW - exposure Y1 - 2014 U6 - https://doi.org/10.1039/c3mt00337j SN - 1756-591X SN - 1756-5901 VL - 2014 IS - 6 SP - 662 EP - 671 ER - TY - JOUR A1 - Pieper, Imke A1 - Wehe, Christoph A. A1 - Bornhorst, Julia A1 - Ebert, Franziska A1 - Leffers, Larissa A1 - Holtkamp, Michael A1 - Hoeseler, Pia A1 - Weber, Till A1 - Mangerich, Aswin A1 - Buerkle, Alexander A1 - Karst, Uwe A1 - Schwerdtle, Tanja T1 - Mechanisms of Hg species induced toxicity in cultured human astrocytes: genotoxicity and DNA-damage response JF - Metallomics : integrated biometal science N2 - The toxicologically most relevant mercury (Hg) species for human exposure is methylmercury (MeHg). Thiomersal is a common preservative used in some vaccine formulations. The aim of this study is to get further mechanistic insight into the yet not fully understood neurotoxic modes of action of organic Hg species. Mercury species investigated include MeHgCl and thiomersal. Additionally HgCl2 was studied, since in the brain mercuric Hg can be formed by dealkylation of the organic species. As a cellular system astrocytes were used. In vivo astrocytes provide the environment necessary for neuronal function. In the present study, cytotoxic effects of the respective mercuricals increased with rising alkylation level and correlated with their cellular bioavailability. Further experiments revealed for all species at subcytotoxic concentrations no induction of DNA strand breaks, whereas all species massively increased H2O2-induced DNA strand breaks. This co- genotoxic effect is likely due to a disturbance of the cellular DNA damage response. Thus, at nanomolar, sub-cytotoxic concentrations, all three mercury species strongly disturbed poly(ADP-ribosyl)ation, a signalling reaction induced by DNA strand breaks. Interestingly, the molecular mechanism behind this inhibition seems to be different for the species. Since chronic PARP-1 inhibition is also discussed to sacrifice neurogenesis and learning abilities, further experiments on neurons and in vivo studies could be helpful to clarify whether the inhibition of poly(ADP-ribosyl) ation contributes to organic Hg induced neurotoxicity. Y1 - 2014 U6 - https://doi.org/10.1039/c3mt00337j SN - 1756-5901 SN - 1756-591X VL - 6 IS - 3 SP - 662 EP - 671 PB - Royal Society of Chemistry CY - Cambridge ER - TY - GEN A1 - Pieper, Imke A1 - Wehe, Christoph A. A1 - Bornhorst, Julia A1 - Ebert, Franziska A1 - Leffers, Larissa A1 - Holtkamp, Michael A1 - Höseler, Pia A1 - Weber, Till A1 - Mangerich, Aswin A1 - Bürkle, Alexander A1 - Karst, Uwe A1 - Schwerdtle, Tanja T1 - Mechanisms of Hg species induced toxicity in cultured human astrocytes BT - genotoxicity and DNA-damage response N2 - The toxicologically most relevant mercury (Hg) species for human exposure is methylmercury (MeHg). Thiomersal is a common preservative used in some vaccine formulations. The aim of this study is to get further mechanistic insight into the yet not fully understood neurotoxic modes of action of organic Hg species. Mercury species investigated include MeHgCl and thiomersal. Additionally HgCl2 was studied, since in the brain mercuric Hg can be formed by dealkylation of the organic species. As a cellular system astrocytes were used. In vivo astrocytes provide the environment necessary for neuronal function. In the present study, cytotoxic effects of the respective mercuricals increased with rising alkylation level and correlated with their cellular bioavailability. Further experiments revealed for all species at subcytotoxic concentrations no induction of DNA strand breaks, whereas all species massively increased H2O2-induced DNA strand breaks. This co- genotoxic effect is likely due to a disturbance of the cellular DNA damage response. Thus, at nanomolar, sub-cytotoxic concentrations, all three mercury species strongly disturbed poly(ADP-ribosyl)ation, a signalling reaction induced by DNA strand breaks. Interestingly, the molecular mechanism behind this inhibition seems to be different for the species. Since chronic PARP-1 inhibition is also discussed to sacrifice neurogenesis and learning abilities, further experiments on neurons and in vivo studies could be helpful to clarify whether the inhibition of poly(ADP-ribosyl) ation contributes to organic Hg induced neurotoxicity. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 171 KW - adduct formation KW - cell-death KW - exposure KW - manganese KW - methylmercury KW - neurodegenerative diseases KW - neurotoxicity KW - poly(ADP-ribose) polymerase-1 KW - repair KW - thimerosal Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-74379 SP - 662 EP - 671 ER - TY - JOUR A1 - Strehlau, Jenny A1 - Weber, Till A1 - Luerenbaum, Constantin A1 - Bornhorst, Julia A1 - Galla, Hans-Joachim A1 - Schwerdtle, Tanja A1 - Winter, Martin A1 - Nowak, Sascha T1 - Towards quantification of toxicity of lithium ion battery electrolytes - development and validation of a liquid-liquid extraction GC-MS method for the determination of organic carbonates in cell culture materials JF - Analytical and bioanalytical chemistry : a merger of Fresenius' journal of analytical chemistry, Analusis and Quimica analitica N2 - A novel method based on liquid-liquid extraction with subsequent gas chromatography separation and mass spectrometric detection (GC-MS) for the quantification of organic carbonates in cell culture materials is presented. Method parameters including the choice of extraction solvent, of extraction method and of extraction time were optimised and the method was validated. The setup allowed for determination within a linear range of more than two orders of magnitude. The limits of detection (LODs) were between 0.0002 and 0.002 mmol/L and the repeatability precisions were in the range of 1.5-12.9%. It could be shown that no matrix effects were present and recovery rates between 98 and 104% were achieved. The methodology was applied to cell culture models incubated with commercial lithium ion battery (LIB) electrolytes to gain more insight into the potential toxic effects of these compounds. The stability of the organic carbonates in cell culture medium after incubation was studied. In a porcine model of the blood-cerebrospinal fluid (CSF) barrier, it could be shown that a transfer of organic carbonates into the brain facing compartment took place. KW - Liquid-liquid extraction KW - GC-MS KW - Lithiumion battery (LIB) KW - Organic carbonates KW - Cell culture materials Y1 - 2017 U6 - https://doi.org/10.1007/s00216-017-0549-6 SN - 1618-2642 SN - 1618-2650 VL - 409 SP - 6123 EP - 6131 PB - Springer CY - Heidelberg ER -