TY  - JOUR
A1  - Schulz, Eike C.
A1  - Mehrabi, Pedram
A1  - Müller-Werkmeister, Henrike
A1  - Tellkamp, Friedjof
A1  - Jha, Ajay
A1  - Stuart, William
A1  - Persch, Elke
A1  - De Gasparo, Raoul
A1  - Diederich, François
A1  - Pai, Emil F.
A1  - Miller, R. J. Dwayne
T1  - The hit-and-return system enables efficient time-resolved serial synchrotron crystallography
JF  - Nature methods : techniques for life scientists and chemists
N2  - We present a ‘hit-and-return’ (HARE) method for time-resolved serial synchrotron crystallography with time resolution from milliseconds to seconds or longer. Timing delays are set mechanically, using the regular pattern in fixed-target crystallography chips and a translation stage system. Optical pump-probe experiments to capture intermediate structures of fluoroacetate dehalogenase binding to its ligand demonstrated that data can be collected at short (30 ms), medium (752 ms) and long (2,052 ms) intervals.
KW  - Biophysical chemistry
KW  - Enzymes
KW  - Molecular biophysics
KW  - X-ray crystallography
Y1  - 2018
U6  - https://doi.org/10.1038/s41592-018-0180-2
SN  - 1548-7091
SN  - 1548-7105
VL  - 15
IS  - 11
SP  - 901
EP  - 904
PB  - Nature Publishing Group (London)
CY  - London
ER  - 
TY  - JOUR
A1  - Darwall, William
A1  - Bremerich, Vanessa
A1  - De Wever, Aaike
A1  - Dell, Anthony I.
A1  - Freyhof, Joerg
A1  - Gessner, Mark O.
A1  - Grossart, Hans-Peter
A1  - Harrison, Ian
A1  - Irvine, Ken
A1  - Jähnig, Sonja C.
A1  - Jeschke, Jonathan M.
A1  - Lee, Jessica J.
A1  - Lu, Cai
A1  - Lewandowska, Aleksandra M.
A1  - Monaghan, Michael T.
A1  - Nejstgaard, Jens C.
A1  - Patricio, Harmony
A1  - Schmidt-Kloiber, Astrid
A1  - Stuart, Simon N.
A1  - Thieme, Michele
A1  - Tockner, Klement
A1  - Turak, Eren
A1  - Weyl, Olaf
T1  - The alliance for freshwater life
BT  - a global call to unite efforts for freshwater biodiversity science and conservation
JF  - Aquatic Conservation: Marine and Freshwater Ecosystems
N2  - 1. Global pressures on freshwater ecosystems are high and rising. Viewed primarily as a resource for humans, current practices of water use have led to catastrophic declines in freshwater species and the degradation of freshwater ecosystems, including their genetic and functional diversity. Approximately three-quarters of the world's inland wetlands have been lost, one-third of the 28 000 freshwater species assessed for the International Union for Conservation of Nature (IUCN) Red List are threatened with extinction, and freshwater vertebrate populations are undergoing declines that are more rapid than those of terrestrial and marine species. This global loss continues unchecked, despite the importance of freshwater ecosystems as a source of clean water, food, livelihoods, recreation, and inspiration.

2.  The causes of these declines include hydrological alterations, habitat degradation and loss, overexploitation, invasive species, pollution, and the multiple impacts of climate change. Although there are policy initiatives that aim to protect freshwater life, these are rarely implemented with sufficient conviction and enforcement. Policies that focus on the development and management of fresh waters as a resource for people almost universally neglect the biodiversity that they contain.

3. Here we introduce the Alliance for Freshwater Life, a global initiative, uniting specialists in research, data synthesis, conservation, education and outreach, and policymaking. This expert network aims to provide the critical mass required for the effective representation of freshwater biodiversity at policy meetings, to develop solutions balancing the needs of development and conservation, and to better convey the important role freshwater ecosystems play in human well-being. Through this united effort we hope to reverse this tide of loss and decline in freshwater biodiversity. We introduce several short- and medium-term actions as examples for making positive change, and invite individuals, organizations, authorities, and governments to join the Alliance for Freshwater Life.
KW  - biodiversity
KW  - conservation evaluation
KW  - endangered species
KW  - fish
KW  - invertebrates
KW  - macrophytes
Y1  - 2018
U6  - https://doi.org/10.1002/aqc.2958
SN  - 1052-7613
SN  - 1099-0755
VL  - 28
IS  - 4
SP  - 1015
EP  - 1022
PB  - Wiley
CY  - Hoboken
ER  -