TY - JOUR A1 - Gerber, Markus A1 - Ehrbar, Janine A1 - Brand, Ralf A1 - Antoniewicz, Franziska A1 - Brand, Serge A1 - Colledge, Flora A1 - Donath, Lars A1 - Egger, Stephan T. A1 - Hatzinger, Martin A1 - Holsboer-Trachsler, Edith A1 - Imboden, Christian A1 - Schweinfurth, Nina A1 - Vetter, Stefan A1 - Ludyga, Sebastian T1 - Implicit attitudes towards exercise and physical activity behaviour among in-patients with psychiatric disorders JF - Mental Health and Physical Activity N2 - The current body of evidence suggests that in healthy participants, implicit attitudes towards physical activity explain variance in exercise behaviour beyond explicit cognitive processes. However, such relationships have not been examined in psychiatric patients, although this may contribute to a better understanding of the motivational and volitional resources needed to self-regulate their exercise behaviour. Therefore, the present cross-sectional study aimed to assess implicit attitudes towards exercise among psychiatric in-patients, and to correlate these implicit attitudes with their physical activity levels. Patients (N = 101) showing a psychiatric disorder, but no severe cognitive impairment, were directly recruited from psychiatric clinics. Their physical activity levels were assessed using both accelerometers and self-reports. Additionally, patients reported psychiatric symptoms and performed a single-target implicit association test (ST-IAT) with exercise employed as the target category. Of all patients, 39% showed a preference for exercise, whereas 13% showed an aversion towards exercise. The implicit attitudes of the remaining participants were equally strong for both concepts. Based on correlational analysis (correcting for age, sex, psychiatric symptoms severity, and ST-IAT sequence), no association was found between ST-IAT score, or self-reported and objectively assessed physical activity. Consequently, the link between exercise behaviour and implicit attitudes towards physical activity found in healthy participants could not be observed in psychiatric patients. KW - Accelerometry KW - Automatic evaluation KW - Dual mode theory KW - Physical activity Y1 - 2018 U6 - https://doi.org/10.1016/j.mhpa.2018.08.001 SN - 1755-2966 VL - 15 SP - 71 EP - 77 PB - Elsevier CY - Oxford ER - TY - JOUR A1 - Asche, Matthias A1 - Gerber, Stefan ED - Dunphy, Graeme ED - Gow, Andrew T1 - Student association JF - Encyclopedia of Early Modern History. Seven Year's War. Symbolic Money Y1 - 2022 SN - 978-90-04-26991-0 VL - 13 PB - Brill CY - Leiden ER - TY - JOUR A1 - Stoffel, Dominik A1 - Kunz, Wolfgang A1 - Gerber, Stefan T1 - And/Or reasoning graphs for determining prime implicants in multi-level combinational networks Y1 - 1997 ER - TY - THES A1 - Gerber, Stefan T1 - Using software for fault detection in arithmetical circuits Y1 - 1995 ER - TY - JOUR A1 - Asche, Matthias A1 - Gerber, Stefan T1 - Universität JF - Historische Bildungsforschung. Konzepte - Methoden - Forschungsfelder KW - Universitätsgeschichte Y1 - 2021 SN - 978-3-8252-5563-3 SN - 978-3-8385-5563-8 SP - 299 EP - 312 PB - Klinkhardt CY - Bad Heilbrunn ER - TY - JOUR A1 - Schmidt, Carsten A1 - Schierack, Peter A1 - Gerber, Ulrike A1 - Schroeder, Christian A1 - Choi, Youngeun A1 - Bald, Ilko A1 - Lehmann, Werner A1 - Rödiger, Stefan T1 - Streptavidin homologues for applications on solid surfaces at high temperatures JF - Langmuir N2 - One of the most commonly used bonds between two biomolecules is the bond between biotin and streptavidin (SA) or streptavidin homologues (SAHs). A high dissociation constant and the consequent high-temperature stability even allows for its use in nucleic acid detection under polymerase chain reaction (PCR) conditions. There are a number of SAHs available, and for assay design, it is of great interest to determine as to which SAH will perform the best under assay conditions. Although there are numerous single studies on the characterization of SAHs in solution or selected solid phases, there is no systematic study comparing different SAHs for biomolecule-binding, hybridization, and PCR assays on solid phases. We compared streptavidin, core streptavidin, traptavidin, core traptavidin, neutravidin, and monomeric streptavidin on the surface of microbeads (10-15 mu m in diameter) and designed multiplex microbead-based experiments and analyzed simultaneously the binding of biotinylated oligonucleotides and the hybridization of oligonucleotides to complementary capture probes. We also bound comparably large DNA origamis to capture probes on the microbead surface. We used a real-time fluorescence microscopy imaging platform, with which it is possible to subject samples to a programmable time and temperature profile and to record binding processes on the microbead surface depending on the time and temperature. With the exception of core traptavidin and monomeric streptavidin, all other SA/SAHs were suitable for our investigations. We found hybridization efficiencies close to 100% for streptavidin, core streptavidin, traptavidin, and neutravidin. These could all be considered equally suitable for hybridization, PCR applications, and melting point analysis. The SA/SAH-biotin bond was temperature sensitive when the oligonucleotide was mono-biotinylated, with traptavidin being the most stable followed by streptavidin and neutravidin. Mono-biotinylated oligonucleotides can be used in experiments with temperatures up to 70 degrees C. When oligonucleotides were bis-biotinylated, all SA/SAH-biotin bonds had similar temperature stability under PCR conditions, even if they comprised a streptavidin variant with slower biotin dissociation and increased mechanostability. Y1 - 2020 U6 - https://doi.org/10.1021/acs.langmuir.9b02339 SN - 0743-7463 VL - 36 IS - 2 SP - 628 EP - 636 PB - American Chemical Society CY - Washington ER - TY - JOUR A1 - Gerber, Stefan A1 - Gössel, Michael T1 - Detection of permanent faults of a floating point adder by pseudoduplication Y1 - 1994 ER -