TY  - JOUR
A1  - Wang, Wei-Hong
A1  - Köhler, Barbara
A1  - Cao, Feng-Qiu
A1  - Liu, Guo-Wei
A1  - Gong, Yuan-Yong
A1  - Sheng, Song
A1  - Song, Qi-Chao
A1  - Cheng, Xiao-Yuan
A1  - Garnett, Trevor
A1  - Okamoto, Mamoru
A1  - Qin, Rui
A1  - Müller-Röber, Bernd
A1  - Tester, Mark
A1  - Liu, Lai-Hua
T1  - Rice DUR3 mediates high-affinity urea transport and plays an effective role in improvement of urea acquisition and utilization when expressed in Arabidopsis
JF  - New phytologist : international journal of plant science
N2  - Despite the great agricultural and ecological importance of efficient use of urea-containing nitrogen fertilizers by crops, molecular and physiological identities of urea transport in higher plants have been investigated only in Arabidopsis. We performed short-time urea-influx assays which have identified a low-affinity and high-affinity (Km of 7.55 mu M) transport system for urea-uptake by rice roots (Oryza sativa). A high-affinity urea transporter OsDUR3 from rice was functionally characterized here for the first time among crops. OsDUR3 encodes an integral membrane-protein with 721 amino acid residues and 15 predicted transmembrane domains. Heterologous expression demonstrated that OsDUR3 restored yeast dur3-mutant growth on urea and facilitated urea import with a Km of c. 10 mu M in Xenopus oocytes. Quantitative reverse-transcription polymerase chain reaction (qPCR) analysis revealed upregulation of OsDUR3 in rice roots under nitrogen-deficiency and urea-resupply after nitrogen-starvation. Importantly, overexpression of OsDUR3 complemented the Arabidopsis atdur3-1 mutant, improving growth on low urea and increasing root urea-uptake markedly. Together with its plasma membrane localization detected by green fluorescent protein (GFP)-tagging and with findings that disruption of OsDUR3 by T-DNA reduces rice growth on urea and urea uptake, we suggest that OsDUR3 is an active urea transporter that plays a significant role in effective urea acquisition and utilisation in rice.
KW  - high-affinity transporter
KW  - leaf senescence
KW  - nitrogen remobilization
KW  - OsDUR3
KW  - overexpression
KW  - rice plant
KW  - urea transport and utilization
Y1  - 2012
U6  - https://doi.org/10.1111/j.1469-8137.2011.03929.x
SN  - 0028-646X
VL  - 193
IS  - 2
SP  - 432
EP  - 444
PB  - Wiley-Blackwell
CY  - Malden
ER  - 
TY  - GEN
A1  - Basler, Nikolas
A1  - Xenikoudakis, Georgios
A1  - Westbury, Michael V.
A1  - Song, Lingfeng
A1  - Sheng, Guilian
A1  - Barlow, Axel
T1  - Reduction of the contaminant fraction of DNA obtained from an ancient giant panda bone
T2  - Postprints der Universität Potsdam Mathematisch-Naturwissenschaftliche Reihe
N2  - Objective:  A key challenge in ancient DNA research is massive microbial DNA contamination from the deposition site which accumulates post mortem in the study organism’s remains. Two simple and cost-effective methods to enrich the relative endogenous fraction of DNA in ancient samples involve treatment of sample powder with either bleach or Proteinase K pre-digestion prior to DNA extraction. Both approaches have yielded promising but vary-ing results in other studies. Here, we contribute data on the performance of these methods using a comprehensive and systematic series of experiments applied to a single ancient bone fragment from a giant panda (Ailuropoda melanoleuca).Results:  Bleach and pre-digestion treatments increased the endogenous DNA content up to ninefold. However, the absolute amount of DNA retrieved was dramatically reduced by all treatments. We also observed reduced DNA damage patterns in pre-treated libraries compared to untreated ones, resulting in longer mean fragment lengths and reduced thymine over-representation at fragment ends. Guanine–cytosine (GC) contents of both mapped and total reads are consistent between treatments and conform to general expectations, indicating no obvious biasing effect of the applied methods. Our results therefore confirm the value of bleach and pre-digestion as tools in palaeog-enomic studies, providing sufficient material is available.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 715 
KW  - ancient DNA (aDNA)
KW  - bleach
KW  - pre-digestion
KW  - endogenous content
KW  - palaeogenomics
KW  - paleogenomics
KW  - next generation sequencing (NGS)
KW  - giant panda
KW  - Ailuropoda melanoleuca
Y1  - 2017
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-428151
SN  - 1866-8372
IS  - 715
ER  -