TY - JOUR A1 - Friess, Fabian A1 - Roch, Toralf A1 - Seifert, Barbara A1 - Lendlein, Andreas A1 - Wischke, Christian T1 - Phagocytosis of spherical and ellipsoidal micronetwork colloids from crosslinked poly(epsilon-caprolactone) JF - International Journal of Pharmaceutics N2 - The effect of non-spherical particle shapes on cellular uptake has been reported as a general design parameter to control cellular recognition of particulate drug carriers. Beside shape, also size and cell-particle ratio should mutually effect phagocytosis. Here, the capability to control cellular uptake of poly(epsilon-caprolactone) (PCL) based polymer micronetwork colloids (MNC), a carrier system that can be transferred to various shapes, is explored in vitro at test conditions allowing multiple cell-particle contacts. PCL-based MNC were synthesized as spheres with a diameter of similar to 6, similar to 10, and 13 mu m, loaded with a fluorescent dye by a specific technique of swelling, redispersion and drying, and transferred into different ellipsoidal shapes by a phantom stretching method. The boundaries of MNC deformability to prolate ellipsoid target shapes were systematically analyzed and found to be at an aspect ratio AR of similar to 4 as obtained by a phantom elongation epsilon(ph) of similar to 150%. Uptake studies with a murine macrophages cell line showed shape dependency of phagocytosis for selected conditions when varying particle sizes (similar to 6 and 10 mu m),and shapes (epsilon(ph): 0, 75 or 150%), cell-particle ratios (1:1, 1:2, 1:10, 1:50), and time points (1-24 h). For larger-sized MNC, there was no significant shape effect on phagocytosis as these particles may associate with more than one cell, thus increasing the possibility of phagocytosis by any of these cells. Accordingly, controlling shape effects on phagocytosis for carriers made from degradable polymers relevant for medical applications requires considering further parameters besides shape, such as kinetic aspects of the exposure and uptake by cells. KW - Particle shape KW - Phagocytosis KW - Macrophage KW - Polymer micronetwork colloids KW - Poly(epsilon-caprolactone) Y1 - 2019 U6 - https://doi.org/10.1016/j.ijpharm.2019.118461 SN - 0378-5173 SN - 1873-3476 VL - 567 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Schwab, Susanne A1 - Seifert, Susanne A1 - Watko, Petra A1 - Obendrauf, Tanja A1 - Trauntschnig, Mike A1 - Gasteiger-Klicpera, Barbara ED - Fritzsche, Tom ED - Meyer, Corinna B. ED - Adelt, Anne ED - Roß, Jennifer T1 - LARS : Ein differenziertes Sprach- und Leseförderprogramm bei GrundschülerInnen mit Deutsch als Erst- und Zweit-Sprache BT - Evaluierung der Pilotstudie JF - Spektrum Patholinguistik N2 - 1 Theoretischer Hintergrund 2 Fragestellung 3 Untersuchungsmethode 4 Ergebnisse 5 Ausblick 6 Literatur Y1 - 2013 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-68544 SN - 1866-9085 SN - 1866-9433 IS - 6 SP - 175 EP - 182 PB - Universitätsverlag Potsdam CY - Potsdam ER - TY - JOUR A1 - Clahsen, Harald A1 - Siegmüller, Julia A1 - Penke, Martina A1 - Schröder, Astrid A1 - Hofmann, Janine A1 - Holzgrefe-Lang, Julia A1 - Skerra, Antje A1 - Adani, Flavia A1 - Gagarina, Natalʹja Vladimirovna A1 - Schröter, Carolin A1 - Frieg, Hendrike A1 - Belke, Eva A1 - Schwab, Susanne A1 - Seifert, Susanne A1 - Watko, Petra A1 - Obendrauf, Tanja A1 - Trauntschnig, Mike A1 - Gasteiger-Klicpera, Barbara A1 - Adelt, Anne A1 - Hanne, Sandra A1 - Burchert, Frank A1 - Swietza, Romy A1 - Doppelbauer, Lea A1 - Dralle, Jenny A1 - Purat, Patricia A1 - Webersinke, Dorothea A1 - Schwytay, Jeannine A1 - Stadie, Nicole A1 - Hoppe, Carina A1 - Heide, Judith A1 - Marusch, Tina A1 - von der Malsburg, Titus Raban A1 - Bastiaanse, Roelien A1 - Schultheiss, Corinna A1 - Nahrstaedt, Holger A1 - Schauer, Thomas A1 - Seidl, Rainer Ottis A1 - Rath, Elisa ED - Fritzsche, Tom ED - Meyer, Corinna B. ED - Adelt, Anne ED - Roß, Jennifer T1 - Spektrum Patholinguistik = Schwerpunktthema: Labyrinth Grammatik: Therapie von syntaktischen Störungen bei Kindern und Erwachsenen N2 - Das Herbsttreffen Patholinguistik wird seit 2007 jährlich vom Verband für Patholinguistik e.V. (vpl) durchgeführt. Das 6. Herbsttreffen mit dem Schwerpunktthema "Labyrinth Grammatik: Therapie von syntaktischen Störungen bei Kindern und Erwachsenen" fand am 17.11.2012 in Potsdam statt. Im vorliegenden Tagungsband finden sich alle Beiträge der Veranstaltung: die vier Hauptvorträge zum Schwerpunkthema, die Vorträge aus Praxis und Forschung von vier Patholinguistinnen in der Reihe Spektrum Patholinguistik sowie die Abstracts der Posterpräsentation. N2 - The 'Herbsttreffen Patholinguistik' is an annual conference organized by the Association for Patholinguistics (Verband für Patholinguistik e.V./vpl) since 2007. The 6th Herbsttreffen with its theme "Labyrinth Grammar: Therapy of Syntactic Disorders in Children and Adults" took place on November 17th, 2012 in Potsdam. These proceedings contain all contributions of the meeting: the four keynote talks, the talks on speech/language therapy and research in the section "Spektrum Patholinguistik" by four patholinguists as well as the abstracts of the poster session. T3 - Spektrum Patholinguistik - 6 KW - Patholinguistik KW - syntaktische Störungen KW - Aphasie KW - Agrammatismus KW - Dysgrammatismus KW - patholinguistics KW - speech/language therapy KW - syntactic disorders KW - aphasia KW - agrammatism Y1 - 2013 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-67659 SN - 978-3-86956-270-4 SN - 1869-3822 SN - 1866-9433 IS - 6 PB - Universitätsverlag Potsdam CY - Potsdam ER -