TY  - JOUR
A1  - Kluth, Oliver
A1  - Stadion, Mandy
A1  - Gottmann, Pascal
A1  - Aga-Barfknecht, Heja
A1  - Jähnert, Markus
A1  - Scherneck, Stephan
A1  - Vogel, Heike
A1  - Krus, Ulrika
A1  - Seelig, Anett
A1  - Ling, Charlotte
A1  - Gerdes, Jantje
A1  - Schürmann, Annette
T1  - Decreased expression of cilia genes in pancreatic islets as a risk factor for type 2 diabetes in mice and humans
JF  - Cell reports
N2  - An insufficient adaptive beta-cell compensation is a hallmark of type 2 diabetes (T2D). Primary cilia function as versatile sensory antennae regulating various cellular processes, but their role on compensatory beta-cell replication has not been examined. Here, we identify a significant enrichment of downregulated, cilia-annotated genes in pancreatic islets of diabetes-prone NZO mice as compared with diabetes-resistant B6-ob/ob mice. Among 327 differentially expressed mouse cilia genes, 81 human orthologs are also affected in islets of diabetic donors. Islets of nondiabetic mice and humans show a substantial overlap of upregulated cilia genes that are linked to cell-cycle progression. The shRNA-mediated suppression of KIF3A, essential for ciliogenesis, impairs division of MINE beta cells as well as in dispersed primary mouse and human islet cells, as shown by decreased BrdU incorporation. These findings demonstrate the substantial role of cilia-gene regulation on islet function and T2D risk.
Y1  - 2019
U6  - https://doi.org/10.1016/j.celrep.2019.02.056
SN  - 2211-1247
VL  - 26
IS  - 11
SP  - 3027
EP  - 3036
PB  - Cell Press
CY  - Maryland Heights
ER  -