TY  - JOUR
A1  - Tötzke, Christian
A1  - Manke, Ingo
A1  - Gaiselmann, Gerd
A1  - Bohner, John
A1  - Müller, Bernd R.
A1  - Kupsch, Andreas
A1  - Hentschel, Manfred P.
A1  - Schmidt, Volker
A1  - Banhart, Jens
A1  - Lehnert, Werner
T1  - A dedicated compression device for high resolution X-ray tomography of compressed gas diffusion layers
JF  - Review of scientific instruments : a monthly journal devoted to scientific instruments, apparatus, and techniques
N2  - We present an experimental approach to study the three-dimensional microstructure of gas diffusion layer (GDL) materials under realistic compression conditions. A dedicated compression device was designed that allows for synchrotron-tomographic investigation of circular samples under well-defined compression conditions. The tomographic data provide the experimental basis for stochastic modeling of nonwoven GDL materials. A plain compression tool is used to study the fiber courses in the material at different compression stages. Transport relevant geometrical parameters, such as porosity, pore size, and tortuosity distributions, are exemplarily evaluated for a GDL sample in the uncompressed state and for a compression of 30 vol.%. To mimic the geometry of the flow-field, we employed a compression punch with an integrated channel-rib-profile. It turned out that the GDL material is homogeneously compressed under the ribs, however, much less compressed underneath the channel. GDL fibers extend far into the channel volume where they might interfere with the convective gas transport and the removal of liquid water from the cell. (C) 2015 AIP Publishing LLC.
Y1  - 2015
U6  - https://doi.org/10.1063/1.4918291
SN  - 0034-6748
SN  - 1089-7623
VL  - 86
IS  - 4
PB  - American Institute of Physics
CY  - Melville
ER  - 
TY  - JOUR
A1  - Schmidt, Carsten
A1  - Schierack, Peter
A1  - Gerber, Ulrike
A1  - Schroeder, Christian
A1  - Choi, Youngeun
A1  - Bald, Ilko
A1  - Lehmann, Werner
A1  - Rödiger, Stefan
T1  - Streptavidin homologues for applications on solid surfaces at high temperatures
JF  - Langmuir
N2  - One of the most commonly used bonds between two biomolecules is the bond between biotin and streptavidin (SA) or streptavidin homologues (SAHs). A high dissociation constant and the consequent high-temperature stability even allows for its use in nucleic acid detection under polymerase chain reaction (PCR) conditions. There are a number of SAHs available, and for assay design, it is of great interest to determine as to which SAH will perform the best under assay conditions. Although there are numerous single studies on the characterization of SAHs in solution or selected solid phases, there is no systematic study comparing different SAHs for biomolecule-binding, hybridization, and PCR assays on solid phases. We compared streptavidin, core streptavidin, traptavidin, core traptavidin, neutravidin, and monomeric streptavidin on the surface of microbeads (10-15 mu m in diameter) and designed multiplex microbead-based experiments and analyzed simultaneously the binding of biotinylated oligonucleotides and the hybridization of oligonucleotides to complementary capture probes. We also bound comparably large DNA origamis to capture probes on the microbead surface. We used a real-time fluorescence microscopy imaging platform, with which it is possible to subject samples to a programmable time and temperature profile and to record binding processes on the microbead surface depending on the time and temperature. With the exception of core traptavidin and monomeric streptavidin, all other SA/SAHs were suitable for our investigations. We found hybridization efficiencies close to 100% for streptavidin, core streptavidin, traptavidin, and neutravidin. These could all be considered equally suitable for hybridization, PCR applications, and melting point analysis. The SA/SAH-biotin bond was temperature sensitive when the oligonucleotide was mono-biotinylated, with traptavidin being the most stable followed by streptavidin and neutravidin. Mono-biotinylated oligonucleotides can be used in experiments with temperatures up to 70 degrees C. When oligonucleotides were bis-biotinylated, all SA/SAH-biotin bonds had similar temperature stability under PCR conditions, even if they comprised a streptavidin variant with slower biotin dissociation and increased mechanostability.
Y1  - 2020
U6  - https://doi.org/10.1021/acs.langmuir.9b02339
SN  - 0743-7463
VL  - 36
IS  - 2
SP  - 628
EP  - 636
PB  - American Chemical Society
CY  - Washington
ER  - 
TY  - JOUR
A1  - Broscheit, Frank
A1  - Bütow, Heike
A1  - Gehrke, Albrecht
A1  - Jahn, Gert
A1  - Jürgens, Christoph
A1  - Kißner, Karl-Heinz
A1  - Klohn, Werner
A1  - Knitschky, Wolfgang
A1  - Mager, Franz-Peter
A1  - Maier, Klaus
A1  - Mühlberger, Wolfgang
A1  - Mülders, Wolfgang
A1  - Rappen-Röhlen, Beate
A1  - Reinhardt, Karl Heinz
A1  - Schmidt, Marianne
A1  - Schmidtke, Kurt-Dietmar
A1  - Volkmann, Hartmut
A1  - Wetzel, Jürgen
T1  - Seydlitz Erdkunde 1 : Ausgabe für Realschulen in Nordrhein-Westfalen
Y1  - 1994
PB  - Schroedel
CY  - Hannover
ER  - 
TY  - BOOK
A1  - Beetz, Petra
A1  - Gering, Karin
A1  - Henning, Gudrun
A1  - Ilffert, Hanelore
A1  - Isensee, Werner
A1  - Klose, Dagmar
A1  - Knothe, Hanna
A1  - Kranz, Sabine
A1  - Osburg, Florian
A1  - Sapparth, Henry
A1  - Schmidt, Gundolf
ED  - Klose, Dagmar
T1  - Expedition Geschichte : Ausg. Brandenburg, Mecklenburg-Vorpommern, Schleswig-Holstein, Hamburg, Rheinland- Pfalz, Sachsen-Anhalt, Berlin ; Gesamtschule, Hauptschule, Realschule ; Bd. 1 Von der Urzeit bis zum römischen Kaiserreich [Schülerarbeitsheft 1]
Y1  - 1998
SN  - 3-425-93381-7
VL  - 1
PB  - Diesterweg
CY  - Frankfurt am Main
ER  - 
TY  - JOUR
A1  - Schmidt, Bernd
A1  - Werner, Frank
A1  - Kelling, Alexandra
A1  - Schilde, Uwe
T1  - The reaction of 3,4-dihydro-2H-pyran with oxalyl chloride : formation and crystal structure analysis of an unexpected bicyclic product
N2  - 3,4-Dihydro-2-H-pyran and oxalyl chloride react, depending on the conditions, to keto esters, a pyran-3- carboxylic acid or derivatives thereof, or to an hitherto unknown bicyclic acetal containing a vinyl chloride moiety. The structure of the latter product has been unambiguously elucidated by single-crystal X-ray structure analysis. A mechanism for its formation is proposed.
Y1  - 2010
UR  - 1960 = dx.doi.org/10.1002/jhet.456
SN  - 0022-152X
ER  - 
TY  - JOUR
A1  - Broscheit, Frank
A1  - Bütow, Martin
A1  - Englert, Wolfgang
A1  - Jahn, Gert
A1  - Klohn, Werner
A1  - Knitschky, Wolfgang
A1  - Reinecke, Hans-Joachim
A1  - Müller, Helmut
A1  - Reinhardt, Karl Heinz
A1  - Schmidt, Marianne
A1  - Schmidtke, Kurt-Dietmar
A1  - Schöpflin, F.
A1  - Starke, Rainer
A1  - Vahldiek, B. W.
A1  - Wehrs, Klaus
A1  - Wetzel, Jürgen
T1  - Seydlitz Erdkunde 2
Y1  - 1996
PB  - Schroedel
CY  - Hannover
ER  - 
TY  - JOUR
A1  - Schmidt, Birgit Angelika
A1  - Ziemann, Martin Andreas
A1  - Pentzien, Simone
A1  - Gabsch, Toralf
A1  - Koch, Werner
A1  - Krüger, Jörg
T1  - Technical analysis of a Central Asian wall painting detached from a Buddhist cave temple on the northern Silk Road
JF  - Studies in Conservation
N2  - A great number of Central Asian wall paintings, archeological materials, architectural fragments, and textiles, as well as painting fragments on silk and paper, make up the so called Turfan Collection at the Asian Art Museum in Berlin. The largest part of the collection comes from the Kucha region, a very important cultural center in the third to ninth centuries. Between 1902 and 1914, four German expeditions traveled along the northern Silk Road. During these expeditions, wall paintings were detached from their original settings in Buddhist cave complexes. This paper reports a technical study of a wall painting, existing in eight fragments, from the Buddhist cave no. 40 (Ritterhohle). Its original painted surface is soot blackened and largely illegible. Gruwedel, leader of the first and third expeditions, described the almost complete destruction of the rediscovered temple complex and evidence of fire damage. The aim of this case study is to identify the materials used for the wall paintings. Furthermore, soot deposits as well as materials from conservation interventions were of interest. Non-invasive analyses were preferred but a limited number of samples were taken to provide more precise information on the painting technique. By employing optical and scanning electron microscopy, energy dispersive X-ray spectroscopy, micro X-ray fluorescence spectroscopy, X-ray diffraction analysis, and Raman spectroscopy, a layer sequence of earthen render, a ground layer made of gypsum, and a paint layer containing a variety of inorganic pigments were identified.
KW  - Wall paintings
KW  - Central Asia
KW  - Silk Road
KW  - Pigments
KW  - Microscopy
KW  - EDX
KW  - XRF
KW  - Raman spectroscopy
Y1  - 2016
U6  - https://doi.org/10.1179/2047058414Y.0000000152
SN  - 0039-3630
SN  - 2047-0584
VL  - 61
SP  - 113
EP  - 122
PB  - Routledge, Taylor & Francis Group
CY  - Abingdon
ER  - 
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A1  - Conrad, Jan
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A1  - Couturier, S.
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A1  - Crimi, G.
A1  - Criswell, S. J.
A1  - Croston, J.
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A1  - Dale, O.
A1  - Daniel, M.
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A1  - Lopez, R. de los Reyes
A1  - De Lotto, B.
A1  - De Luca, A.
A1  - de Mello Neto, J. R. T.
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A1  - de Oliveira, Y.
A1  - de Ona Wilhelmi, E.
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A1  - Decock, G.
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A1  - Dubois, R.
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A1  - Egberts, Kathrin
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A1  - Khelifi, B.
A1  - Kieda, D. B.
A1  - Kifune, T.
A1  - Kihm, T.
A1  - Kishimoto, T.
A1  - Kitamoto, K.
A1  - Kluzniak, W.
A1  - Knapic, C.
A1  - Knapp, J. w
A1  - Knoedlseder, J.
A1  - Koeck, F.
A1  - Kocot, J.
A1  - Kodani, K.
A1  - Koehne, J. -H.
A1  - Kohri, K.
A1  - Kokkotas, K.
A1  - Kolitzus, D.
A1  - Komin, N.
A1  - Kominis, I.
A1  - Konno, Y.
A1  - Koeppel, H.
A1  - Korohoda, P.
A1  - Kosack, K.
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T1  - Introducing the CTA concept
T2  - Astroparticle physics
N2  - The Cherenkov Telescope Array (CTA) is a new observatory for very high-energy (VHE) gamma rays. CTA has ambitions science goals, for which it is necessary to achieve full-sky coverage, to improve the sensitivity by about an order of magnitude, to span about four decades of energy, from a few tens of GeV to above 100 TeV with enhanced angular and energy resolutions over existing VHE gamma-ray observatories. An international collaboration has formed with more than 1000 members from 27 countries in Europe, Asia, Africa and North and South America. In 2010 the CTA Consortium completed a Design Study and started a three-year Preparatory Phase which leads to production readiness of CTA in 2014. In this paper we introduce the science goals and the concept of CTA, and provide an overview of the project.
KW  - TeV gamma-ray astronomy
KW  - Air showers
KW  - Cherenkov Telescopes
Y1  - 2013
U6  - https://doi.org/10.1016/j.astropartphys.2013.01.007
SN  - 0927-6505
SN  - 1873-2852
VL  - 43
IS  - 2
SP  - 3
EP  - 18
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - GEN
A1  - Wojcinski, Sebastian
A1  - Dupont, Jennifer
A1  - Schmidt, Werner
A1  - Cassel, Michael
A1  - Hillemanns, Peter
T1  - Real-time ultrasound elastography in 180 axillary lymph nodes
BT  - elasticity distribution in healthy lymph nodes and prediction of breast cancer metastases
T2  - Postprints der Universität Potsdam : Humanwissenschaftliche Reihe
N2  - Background

To determine the general appearance of normal axillary lymph nodes (LNs) in real-time tissue sonoelastography and to explore the method′s potential value in the prediction of LN metastases.

Methods

Axillary LNs in healthy probands (n=165) and metastatic LNs in breast cancer patients (n=15) were examined with palpation, B-mode ultrasound, Doppler and sonoelastography (assessment of the elasticity of the cortex and the medulla). The elasticity distributions were compared and sensitivity (SE) and specificity (SP) were calculated. In an exploratory analysis, positive and negative predictive values (PPV, NPV) were calculated based upon the estimated prevalence of LN metastases in different risk groups.

Results

In the elastogram, the LN cortex was significantly harder than the medulla in both healthy (p=0.004) and metastatic LNs (p=0.005). Comparing healthy and metastatic LNs, there was no difference in the elasticity distribution of the medulla (p=0.281), but we found a significantly harder cortex in metastatic LNs (p=0.006). The SE of clinical examination, B-mode ultrasound, Doppler ultrasound and sonoelastography was revealed to be 13.3%, 40.0%, 14.3% and 60.0%, respectively, and SP was 88.4%, 96.8%, 95.6% and 79.6%, respectively. The highest SE was achieved by the disjunctive combination of B-mode and elastographic features (cortex >3mm in B-mode or blue cortex in the elastogram, SE=73.3%). The highest SP was achieved by the conjunctive combination of B-mode ultrasound and elastography (cortex >3mm in B-mode and blue cortex in the elastogram, SP=99.3%).

Conclusions

Sonoelastography is a feasible method to visualize the elasticity distribution of LNs. Moreover, sonoelastography is capable of detecting elasticity differences between the cortex and medulla, and between metastatic and healthy LNs. Therefore, sonoelastography yields additional information about axillary LN status and can improve the PPV, although this method is still experimental.
T3  - Zweitveröffentlichungen der Universität Potsdam : Humanwissenschaftliche Reihe - 626 
KW  - breast ultrasound
KW  - axillary lymph nodes
KW  - sonoelastography
KW  - real-time tissue elastography
KW  - cancer detection
KW  - elasticity imaging
KW  - HI-RTE
KW  - lymph node metastases
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-431584
SN  - 1866-8364
IS  - 626
ER  - 
TY  - JOUR
A1  - Wojcinski, Sebastian
A1  - Cassel, Michael
A1  - Farrokh, Andre
A1  - Soliman, Amr A.
A1  - Hille, Ursula
A1  - Schmidt, Werner
A1  - Degenhardt, Friedrich
A1  - Hillemanns, Peter
T1  - Variations in the elasticity of breast tissue during the menstrual cycle determined by real-time sonoelastography
JF  - Journal of ultrasound in medicine
N2  - Objectives-The purpose of this study was to determine the dependence of breast tissue elasticity on the menstrual cycle of healthy volunteers by means of real-time sonoelastography.
 Methods-Twenty-two healthy volunteers (aged 18-33 years) were examined once weekly during two consecutive menstrual cycles using sonoelastography. Group 1 (n = 10) was not taking hormonal medication; group 2 (n = 12) was taking oral contraceptives.
 Results-The breast parenchyma appeared softer than the dermis and harder than the adipose tissue, and elasticity varied over the menstrual cycle and between groups. Group 1 (no hormone intake) showed continuously increasing elasticity with relatively soft breast parenchyma in the menstrual and follicular phases and harder parenchyma in the luteal phase (P = .012). Group 2 (oral contraceptives) showed no statistically significant changes in breast parenchymal elasticity according to sonoelastography. The parenchyma was generally softer in group 1 compared with group 2 throughout the menstrual cycle (P = .033). The dermis, the subcutaneous adipose tissue, and the pectoralis major muscle showed no changes in elasticity. Comparison of measurements made during the first and the second menstrual cycles showed similar patterns of elasticity in both groups.
 Conclusions-Sonoelastography is a reproducible method that can be used to determine the dependence of breast parenchyma elasticity on the menstrual cycle and on the intake of hormonal contraceptives.
KW  - breast tissue
KW  - elasticity imaging
KW  - real-time tissue elastography
KW  - sonoelastography
Y1  - 2012
SN  - 0278-4297
SN  - 1550-9613
VL  - 31
IS  - 1
SP  - 63
EP  - 72
PB  - American Institute of Ultrasound in Medicine
CY  - Laurel
ER  - 
TY  - JOUR
A1  - Wojcinski, Sebastian
A1  - Dupont, Jennifer
A1  - Schmidt, Werner
A1  - Cassel, Michael
A1  - Hillemanns, Peter
T1  - Real-time ultrasound elastography in 180 axillary lymph nodes  elasticity distribution in healthy lymph nodes and prediction of breast cancer metastases
JF  - BMC medical imaging
N2  - Background: To determine the general appearance of normal axillary lymph nodes (LNs) in real-time tissue sonoelastography and to explore the method's potential value in the prediction of LN metastases.
 Methods: Axillary LNs in healthy probands (n=165) and metastatic LNs in breast cancer patients (n=15) were examined with palpation, B-mode ultrasound, Doppler and sonoelastography (assessment of the elasticity of the cortex and the medulla). The elasticity distributions were compared and sensitivity (SE) and specificity (SP) were calculated. In an exploratory analysis, positive and negative predictive values (PPV, NPV) were calculated based upon the estimated prevalence of LN metastases in different risk groups.
 Results: In the elastogram, the LN cortex was significantly harder than the medulla in both healthy (p=0.004) and metastatic LNs (p=0.005). Comparing healthy and metastatic LNs, there was no difference in the elasticity distribution of the medulla (p=0.281), but we found a significantly harder cortex in metastatic LNs (p=0.006). The SE of clinical examination, B-mode ultrasound, Doppler ultrasound and sonoelastography was revealed to be 13.3%, 40.0%, 14.3% and 60.0%, respectively, and SP was 88.4%, 96.8%, 95.6% and 79.6%, respectively. The highest SE was achieved by the disjunctive combination of B-mode and elastographic features (cortex >3mm in B-mode or blue cortex in the elastogram, SE=73.3%). The highest SP was achieved by the conjunctive combination of B-mode ultrasound and elastography (cortex >3mm in B-mode and blue cortex in the elastogram, SP=99.3%).
 Conclusions: Sonoelastography is a feasible method to visualize the elasticity distribution of LNs. Moreover, sonoelastography is capable of detecting elasticity differences between the cortex and medulla, and between metastatic and healthy LNs. Therefore, sonoelastography yields additional information about axillary LN status and can improve the PPV, although this method is still experimental.
KW  - Breast ultrasound
KW  - Axillary lymph nodes
KW  - Sonoelastography
KW  - Real-time tissue elastography
KW  - Cancer detection
KW  - Elasticity imaging
KW  - HI-RTE
KW  - Lymph node metastases
Y1  - 2012
U6  - https://doi.org/10.1186/1471-2342-12-35
SN  - 1471-2342
VL  - 12
PB  - BioMed Central
CY  - London
ER  - 
TY  - BOOK
A1  - Schmidt, Thorsten Ingo
A1  - Ulrich, Peter
A1  - Büchner, Christiane
A1  - Franzke, Jochen
A1  - Jann, Werner
A1  - Bauer, Hartmut
A1  - Wagner, Dieter
A1  - Brüning, Christoph
A1  - Bickenbach, Christian
A1  - Kuhlmann, Sabine
A1  - Peters, Niklas
A1  - Reichard, Christoph
A1  - Tessmann, Jens
A1  - Maaß, Christian
A1  - Kern, Kristine
A1  - Kochskämper, Elisa
A1  - Gailing, Ludger
A1  - Krzymuski, Marcin
ED  - Schmidt, Thorsten Ingo
ED  - Bickenbach, Christian
ED  - Gronewold, Ulfert
ED  - Kuhlmann, Sabine
ED  - Ulrich, Peter
T1  - Kommunalwissenschaften an der Universität Potsdam
BT  - Rück- und Ausblick zum 30-jährigen Bestehen des Kommunalwissenschaftlichen Instituts (KWI)
T3  - KWI-Schriften
N2  - Zum dreißigjährigen Bestehen des Kommunalwissenschaftlichen Instituts an der Universität Potsdam vereint dieser Jubiläumsband kurze Aufsätze von ehemaligen und aktuellen Vorstandsmitgliedern, von Ehrenmitgliedern des Vorstands, langjährigen wissenschaftlichen Mitarbeitern des Instituts und aktuellen wissenschaftlichen Kooperationspartnern. Die insgesamt zwölf Beiträge befassen sich mit den Kommunalwissenschaften und der Geschichte des Kommunalwissenschaftlichen Instituts, mit aktuellen kommunalwissenschaftlichen Fragestellungen und wissenschaftlichen Kooperationen des KWI. Der vom KWI-Vorstand herausgegebene Band soll einen breiten Blick auf 30 Jahre Kommunalwissenschaften in Brandenburg und an der Universität Potsdam werfen und einen Ausblick auf zukünftige kommunalwissenschaftliche Forschung geben.
T3  - KWI-Schriften - 15 
KW  - Kommunalwissenschaft
KW  - Institut
KW  - Brandenburg
KW  - Potsdam
KW  - Universität
Y1  - 2024
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-636180
SN  - 978-3-86956-581-1
SN  - 1867-951X
SN  - 1867-9528
IS  - 15
PB  - Universitätsverlag Potsdam
CY  - Potsdam
ER  -