TY  - GEN
A1  - Olmer, Ruth
A1  - Engels, Lena
A1  - Usman, Abdulai
A1  - Menke, Sandra
A1  - Malik, Muhammad Nasir Hayat
A1  - Pessler, Frank
A1  - Göhring, Gudrun
A1  - Bornhorst, Dorothee
A1  - Bolten, Svenja
A1  - Abdelilah-Seyfried, Salim
A1  - Scheper, Thomas
A1  - Kempf, Henning
A1  - Zweigerdt, Robert
A1  - Martin, Ulrich
T1  - Differentiation of Human Pluripotent Stem Cells into Functional Endothelial Cells in Scalable Suspension Culture
T2  - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - Endothelial cells (ECs) are involved in a variety of cellular responses. As multifunctional components of vascular structures, endothelial (progenitor) cells have been utilized in cellular therapies and are required as an important cellular component of engineered tissue constructs and in vitro disease models. Although primary ECs from different sources are readily isolated and expanded, cell quantity and quality in terms of functionality and karyotype stability is limited. ECs derived from human induced pluripotent stem cells (hiPSCs) represent an alternative and potentially superior cell source, but traditional culture approaches and 2D differentiation protocols hardly allow for production of large cell numbers. Aiming at the production of ECs, we have developed a robust approach for efficient endothelial differentiation of hiPSCs in scalable suspension culture. The established protocol results in relevant numbers of ECs for regenerative approaches and industrial applications that show in vitro proliferation capacity and a high degree of chromosomal stability.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1182 
KW  - virus infection
KW  - progenitor cells
KW  - in vitro
KW  - telomere dysfunction
KW  - cord blood
KW  - cardiomyogenic differentiation
KW  - angiogenesis
KW  - efficient
KW  - aberrations
KW  - expression
Y1  - 2017
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-427095
SN  - 1866-8372
IS  - 5
ER  - 
TY  - JOUR
A1  - Olmer, Ruth
A1  - Engels, Lena
A1  - Usman, Abdulai
A1  - Menke, Sandra
A1  - Malik, Muhammad Nasir Hayat
A1  - Pessler, Frank
A1  - Goehring, Gudrun
A1  - Bornhorst, Dorothee
A1  - Bolten, Svenja
A1  - Abdelilah-Seyfried, Salim
A1  - Scheper, Thomas
A1  - Kempf, Henning
A1  - Zweigerdt, Robert
A1  - Martin, Ulrich
T1  - Differentiation of Human Pluripotent Stem Cells into Functional Endothelial Cells in Scalable Suspension Culture
JF  - Stem Cell Reports
N2  - Endothelial cells (ECs) are involved in a variety of cellular responses. As multifunctional components of vascular structures, endothelial (progenitor) cells have been utilized in cellular therapies and are required as an important cellular component of engineered tissue constructs and in vitro disease models. Although primary ECs from different sources are readily isolated and expanded, cell quantity and quality in terms of functionality and karyotype stability is limited. ECs derived from human induced pluripotent stem cells (hiPSCs) represent an alternative and potentially superior cell source, but traditional culture approaches and 2D differentiation protocols hardly allow for production of large cell numbers. Aiming at the production of ECs, we have developed a robust approach for efficient endothelial differentiation of hiPSCs in scalable suspension culture. The established protocol results in relevant numbers of ECs for regenerative approaches and industrial applications that show in vitro proliferation capacity and a high degree of chromosomal stability.
KW  - virus infection
KW  - progenitor cells
KW  - in vitro
KW  - telomere dysfunction
KW  - cord blood
KW  - cardiomyogenic differentiation
KW  - angiogenesis
KW  - efficient
KW  - aberrations
KW  - expression
Y1  - 2017
U6  - https://doi.org/10.1016/j.stemcr.2018.03.017
SN  - 2213-6711
VL  - 10
IS  - 5
PB  - Springer
CY  - New York
ER  -