TY - JOUR A1 - Sperber, Hannah Sabeth A1 - Welke, Robert-William A1 - Petazzi, Roberto Arturo A1 - Bergmann, Ronny A1 - Schade, Matthias A1 - Shai, Yechiel A1 - Chiantia, Salvatore A1 - Herrmann, Andreas A1 - Schwarzer, Roland T1 - Self-association and subcellular localization of Puumala hantavirus envelope proteins JF - Scientific reports N2 - Hantavirus assembly and budding are governed by the surface glycoproteins Gn and Gc. In this study, we investigated the glycoproteins of Puumala, the most abundant Hantavirus species in Europe, using fluorescently labeled wild-type constructs and cytoplasmic tail (CT) mutants. We analyzed their intracellular distribution, co-localization and oligomerization, applying comprehensive live, single-cell fluorescence techniques, including confocal microscopy, imaging flow cytometry, anisotropy imaging and Number&Brightness analysis. We demonstrate that Gc is significantly enriched in the Golgi apparatus in absence of other viral components, while Gn is mainly restricted to the endoplasmic reticulum (ER). Importantly, upon co-expression both glycoproteins were found in the Golgi apparatus. Furthermore, we show that an intact CT of Gc is necessary for efficient Golgi localization, while the CT of Gn influences protein stability. Finally, we found that Gn assembles into higher-order homo-oligomers, mainly dimers and tetramers, in the ER while Gc was present as mixture of monomers and dimers within the Golgi apparatus. Our findings suggest that PUUV Gc is the driving factor of the targeting of Gc and Gn to the Golgi region, while Gn possesses a significantly stronger self-association potential. Y1 - 2019 U6 - https://doi.org/10.1038/s41598-018-36879-y SN - 2045-2322 VL - 9 PB - Nature Publ. Group CY - London ER - TY - JOUR A1 - Schade, Robert T1 - Flammende Prospekte : die Großstadt als Bewußtseinspiel und die Ambivalenz des untergeordneten Raums : Andrej Belyis Petersburg Y1 - 2011 SN - 978-3-8376-1404-6 ER - TY - THES A1 - Schade, Robert T1 - Schwankende Ansichten BT - Zur Geschichte einer Ästhetik des Anders-Sehens in der Literatur und Kunst der Moderne T2 - Metabasis - Transkriptionen zwischen Literaturen, Künsten und Medien ; 19 N2 - Neu sehen, anders sehen - seit dem 19. Jahrhundert hat sich in der Literatur eine Ästhetik alternativer Sehformen etabliert, die die eigene Wahrnehmung zunehmend infrage stellt und sich in der Figur des Schwankens manifestiert. Robert Schade eröffnet einen Einblick in die Geschichte dieser alternativen Sehformen, indem er ausgehend von ökonomischen Wahrnehmungstheorien des 19. und 20. Jahrhunderts Fragestellungen aus Psychologie, Philosophie und Ästhetik verfolgt. Er stellt auf diesem Wege erstmals eine Verbindung zwischen der Sehtheorie Hermann von Helmholtz' und der Verfremdungsästhetik Viktor Shklovskijs her. Am Beispiel von vier Werkuntersuchungen (von Gustave Caillebotte, Andrej Belyj, H.G. Wells und Luigi Pirandello) werden die Vernetztheit und das kritische Potenzial der Figur des Schwankens in unterschiedlichen Diskursen illustriert. Y1 - 2017 SN - 978-3-8376-3993-3 PB - transcript CY - Bielefeld ER - TY - GEN A1 - Sperber, Hannah Sabeth A1 - Welke, Robert-William A1 - Petazzi, Roberto Arturo A1 - Bergmann, Ronny A1 - Schade, Matthias A1 - Shai, Yechiel A1 - Chiantia, Salvatore A1 - Herrmann, Andreas A1 - Schwarzer, Roland T1 - Self-association and subcellular localization of Puumala hantavirus envelope proteins T2 - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe N2 - Hantavirus assembly and budding are governed by the surface glycoproteins Gn and Gc. In this study, we investigated the glycoproteins of Puumala, the most abundant Hantavirus species in Europe, using fluorescently labeled wild-type constructs and cytoplasmic tail (CT) mutants. We analyzed their intracellular distribution, co-localization and oligomerization, applying comprehensive live, single-cell fluorescence techniques, including confocal microscopy, imaging flow cytometry, anisotropy imaging and Number&Brightness analysis. We demonstrate that Gc is significantly enriched in the Golgi apparatus in absence of other viral components, while Gn is mainly restricted to the endoplasmic reticulum (ER). Importantly, upon co-expression both glycoproteins were found in the Golgi apparatus. Furthermore, we show that an intact CT of Gc is necessary for efficient Golgi localization, while the CT of Gn influences protein stability. Finally, we found that Gn assembles into higher-order homo-oligomers, mainly dimers and tetramers, in the ER while Gc was present as mixture of monomers and dimers within the Golgi apparatus. Our findings suggest that PUUV Gc is the driving factor of the targeting of Gc and Gn to the Golgi region, while Gn possesses a significantly stronger self-association potential. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 648 KW - Sin-Nombre-Virus KW - nucleocapsid protein KW - cytoplasmic tails KW - electron cryotomography KW - autophagic clearance KW - glycoprotein KW - Gn KW - G1 KW - brightness KW - fever Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-425040 SN - 1866-8372 IS - 648 ER - TY - CHAP A1 - Schade, Robert T1 - Bizarrerie als Verfahren BT - über das Bild Intérieur, femme lisante Gustave Caillebottes und dessen kunstkritische Kommentare T2 - Visualisierung, Visibilisierung und Verschriftlichung Y1 - 2017 SN - 978-3-938944-93-6 SP - 341 EP - 353 PB - Tranvia CY - Berlin ER -