TY  - GEN
A1  - Nowak, Michael D.
A1  - Russo, Giancarlo
A1  - Schlapbach, Ralph
A1  - Huu, Cuong Nguyen
A1  - Lenhard, Michael
A1  - Conti, Elena
T1  - The draft genome of Primula veris yields insights into the molecular basis of heterostyly
T2  - Postprints der Universität Potsdam : Mathematisch Naturwissenschaftliche Reihe
N2  - Background

The flowering plant Primula veris is a common spring blooming perennial that is widely cultivated throughout Europe. This species is an established model system in the study of the genetics, evolution, and ecology of heterostylous floral polymorphisms. Despite the long history of research focused on this and related species, the continued development of this system has been restricted due the absence of genomic and transcriptomic resources.

Results

We present here a de novo draft genome assembly of P. veris covering 301.8 Mb, or approximately 63% of the estimated 479.22 Mb genome, with an N50 contig size of 9.5 Kb, an N50 scaffold size of 164 Kb, and containing an estimated 19,507 genes. The results of a RADseq bulk segregant analysis allow for the confident identification of four genome scaffolds that are linked to the P. veris S-locus. RNAseq data from both P. veris and the closely related species P. vulgaris allow for the characterization of 113 candidate heterostyly genes that show significant floral morph-specific differential expression. One candidate gene of particular interest is a duplicated GLOBOSA homolog that may be unique to Primula (PveGLO2), and is completely silenced in L-morph flowers.

Conclusions

The P. veris genome represents the first genome assembled from a heterostylous species, and thus provides an immensely important resource for future studies focused on the evolution and genetic dissection of heterostyly. As the first genome assembled from the Primulaceae, the P. veris genome will also facilitate the expanded application of phylogenomic methods in this diverse family and the eudicots as a whole.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 879 
KW  - pollen flow
KW  - reproductive success
KW  - natural-populations
KW  - genetic-variation
KW  - breeding system
KW  - floral morph
KW  - evolution
KW  - vulgaris
KW  - identification
KW  - transcriptome
KW  - Genome Assembly
KW  - Veris
KW  - Transcriptome Assembly
KW  - Corolla Tube
KW  - Genome Scaffold
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-435088
SN  - 1866-8372
IS  - 879
ER  - 
TY  - JOUR
A1  - Nowak, Michael D.
A1  - Russo, Giancarlo
A1  - Schlapbach, Ralph
A1  - Cuong Nguyen Huu, 
A1  - Lenhard, Michael
A1  - Conti, Elena
T1  - The draft genome of Primula veris yields insights into the molecular basis of heterostyly
JF  - Genome biology : biology for the post-genomic era
N2  - Background: The flowering plant Primula veris is a common spring blooming perennial that is widely cultivated throughout Europe. This species is an established model system in the study of the genetics, evolution, and ecology of heterostylous floral polymorphisms. Despite the long history of research focused on this and related species, the continued development of this system has been restricted due the absence of genomic and transcriptomic resources.
 Results: We present here a de novo draft genome assembly of P. veris covering 301.8 Mb, or approximately 63% of the estimated 479.22 Mb genome, with an N50 contig size of 9.5 Kb, an N50 scaffold size of 164 Kb, and containing an estimated 19,507 genes. The results of a RADseq bulk segregant analysis allow for the confident identification of four genome scaffolds that are linked to the P. veris S-locus. RNAseq data from both P. veris and the closely related species P. vulgaris allow for the characterization of 113 candidate heterostyly genes that show significant floral morph-specific differential expression. One candidate gene of particular interest is a duplicated GLOBOSA homolog that may be unique to Primula (PveGLO2), and is completely silenced in L-morph flowers.
 Conclusions: The P. veris genome represents the first genome assembled from a heterostylous species, and thus provides an immensely important resource for future studies focused on the evolution and genetic dissection of heterostyly. As the first genome assembled from the Primulaceae, the P. veris genome will also facilitate the expanded application of phylogenomic methods in this diverse family and the eudicots as a whole.
Y1  - 2015
U6  - https://doi.org/10.1186/s13059-014-0567-z
SN  - 1465-6906
SN  - 1474-760X
VL  - 16
PB  - BioMed Central
CY  - London
ER  -