TY  - JOUR
A1  - Contin, Andrea
A1  - Frasca, Stefano
A1  - Vivekananthan, Jeevanthi
A1  - Leimkühler, Silke
A1  - Wollenberger, Ursula
A1  - Plumere, Nicolas
A1  - Schuhmann, Wolfgang
T1  - A pH Responsive Redox Hydrogel for Electrochemical Detection of Redox Silent Biocatalytic Processes. Control of Hydrogel Solvation
JF  - Electroanalysis : an international journal devoted to fundamental and practical aspects of electroanalysis
N2  - The control of bioelectrocatalytic processes by external stimuli for the indirect detection of non-redox active species was achieved using an esterase and a redox enzyme both integrated within a redox hydrogel. The poly( vinyl) imidazole Os(bpy)(2)Cl hydrogel displays pH-responsive properties. The esterase catalysed reaction leads to a local pH decrease causing protonation of imidazole moieties thus increasing hydrogel solvation and mobility of the tethered Os-complexes. This is the key step to enable improved electron transfer between an aldehyde oxidoreductase and the polymer-bound Os-complexes. The off-on switch is further integrated in a biofuel cell system for self-powered signal generation.
KW  - pH responsive hydrogel
KW  - External stimuli
KW  - Biofuel cell
KW  - Self-powered biosensor
KW  - Solvation
Y1  - 2015
U6  - https://doi.org/10.1002/elan.201400621
SN  - 1040-0397
SN  - 1521-4109
VL  - 27
IS  - 4
SP  - 938
EP  - 944
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Pinyou, Piyanut
A1  - Ruff, Adrian
A1  - Poeller, Sascha
A1  - Barwe, Stefan
A1  - Nebel, Michaela
A1  - Alburquerque, Natalia Guerrero
A1  - Wischerhoff, Erik
A1  - Laschewsky, Andre
A1  - Schmaderer, Sebastian
A1  - Szeponik, Jan
A1  - Plumere, Nicolas
A1  - Schuhmann, Wolfgang
T1  - Thermoresponsive amperometric glucose biosensor
JF  - Biointerphases
N2  - The authors report on the fabrication of a thermoresponsive biosensor for the amperometric detection of glucose. Screen printed electrodes with heatable gold working electrodes were modified by a thermoresponsive statistical copolymer [polymer I: poly(omega-ethoxytriethylenglycol methacrylate-omega-3-(N,N-dimethyl-N-2-methacryloyloxyethyl ammonio) propanesulfonate-co-omega-butoxydiethylenglycol methacrylate-co-2-(4-benzoyl-phenoxy)ethyl methacrylate)] with a lower critical solution temperature of around 28 degrees C in aqueous solution via electrochemically induced codeposition with a pH-responsive redox-polymer [polymer II: poly(glycidyl methacrylate-co-allyl methacrylate-co-poly(ethylene glycol) methacrylate-co-butyl acrylate-co-2-(dimethylamino) ethyl methacrylate)-[Os(bpy)(2)(4-(((2-(2-(2-aminoethoxy) ethoxy) ethyl) amino) methyl)-N,N-dimethylpicolinamide)](2+)] and pyrroloquinoline quinone-soluble glucose dehydrogenase acting as biological recognition element. Polymer II bears covalently bound Os-complexes that act as redox mediators for shuttling electrons between the enzyme and the electrode surface. Polymer I acts as a temperature triggered immobilization matrix. Probing the catalytic current as a function of the working electrode temperature shows that the activity of the biosensor is dramatically reduced above the phase transition temperature of polymer I. Thus, the local modulation of the temperature at the interphase between the electrode and the bioactive layer allows switching the biosensor from an on-to an off-state without heating of the surrounding analyte solution. (C) 2015 American Vacuum Society.
Y1  - 2016
U6  - https://doi.org/10.1116/1.4938382
SN  - 1934-8630
SN  - 1559-4106
VL  - 11
PB  - American Institute of Physics
CY  - Melville
ER  -