TY - JOUR
A1 - Olimi, Expedito
A1 - Kusstatscher, Peter
A1 - Wicaksono, Wisnu Adi
A1 - Abdelfattah, Ahmed
A1 - Cernava, Tomislav
A1 - Berg, Gabriele
T1 - Insights into the microbiome assembly during different growth stages and storage of strawberry plants
JF - Environmental microbiome
N2 - Background: Microbiome assembly was identified as an important factor for plant growth and health, but this process is largely unknown, especially for the fruit microbiome. Therefore, we analyzed strawberry plants of two cultivars by focusing on microbiome tracking during the different growth stages and storage using amplicon sequencing, qPCR, and microscopic approaches.
Results: Strawberry plants carried a highly diverse microbiome, therein the bacterial families Sphingomonadaceae (25%), Pseudomonadaceae (17%), and Burkholderiaceae (11%); and the fungal family Mycosphaerella (45%) were most abundant. All compartments were colonized by high number of bacteria and fungi (10(7)-10(10) marker gene copies per g fresh weight), and were characterized by high microbial diversity (6049 and 1501 ASVs); both were higher for the belowground samples than in the phyllosphere. Compartment type was the main driver of microbial diversity, structure, and abundance (bacterial: 45%; fungal: 61%) when compared to the cultivar (1.6%; 2.2%). Microbiome assembly was strongly divided for belowground habitats and the phyllosphere; only a low proportion of the microbiome was transferred from soil via the rhizosphere to the phyllosphere. During fruit development, we observed the highest rates of microbial transfer from leaves and flowers to ripe fruits, where most of the bacteria occured inside the pulp. In postharvest fruits, microbial diversity decreased while the overall abundance increased. Developing postharvest decay caused by Botrytis cinerea decreased the diversity as well, and induced a reduction of potentially beneficial taxa.
Conclusion: Our findings provide insights into microbiome assembly in strawberry plants and highlight the importance of microbe transfer during fruit development and storage with potential implications for food health and safety.
KW - Fragaria x ananassa
KW - Microbiome assembly
KW - Fruit pathogens
KW - Bacterial
KW - communities
KW - Fungal communities
KW - Amplicon sequencing
KW - CLSM
Y1 - 2022
U6 - https://doi.org/10.1186/s40793-022-00415-3
SN - 2524-6372
VL - 17
IS - 1
PB - BMC
CY - London
ER -
TY - JOUR
A1 - Wassermann, Birgit
A1 - Abdelfattah, Ahmed
A1 - Wicaksono, Wisnu Adi
A1 - Kusstatscher, Peter
A1 - Müller, Henry
A1 - Cernava, Tomislav
A1 - Goertz, Simon
A1 - Rietz, Steffen
A1 - Abbadi, Amine
A1 - Berg, Gabriele
T1 - The Brassica napus seed microbiota is cultivar-specific and transmitted via paternal breeding lines
JF - Microbial biotechnology
N2 - Seed microbiota influence germination and plant health and have the potential to improve crop performance, but the factors that determine their structure and functions are still not fully understood.
Here, we analysed the impact of plant-related and external factors on seed endophyte communities of 10 different oilseed rape (Brassica napus L.) cultivars from 26 field sites across Europe.
All seed lots harboured a high abundance and diversity of endophytes, which were dominated by six genera: Ralstonia, Serratia, Enterobacter, Pseudomonas, Pantoea, and Sphingomonas.
The cultivar was the main factor explaining the variations in bacterial diversity, abundance and composition. In addition, the latter was significantly influenced by diverse biotic and abiotic factors, for example host germination rates and disease resistance against Plasmodiophora brassicae.
A set of bacterial biomarkers was identified to discriminate between characteristics of the seeds, for example Sphingomonas for improved germination and Brevundimonas for disease resistance.
Application of a Bayesian community approach suggested vertical transmission of seed endophytes, where the paternal parent plays a major role and might even determine the germination performance of the offspring.
This study contributes to the understanding of seed microbiome assembly and underlines the potential of the microbiome to be implemented in crop breeding and biocontrol programmes.
Y1 - 2022
U6 - https://doi.org/10.1111/1751-7915.14077
SN - 1751-7915
VL - 15
IS - 9
SP - 2379
EP - 2390
PB - Wiley
CY - Hoboken
ER -