TY  - JOUR
A1  - Dey, Pradip
A1  - Adamovski, Miriam
A1  - Friebe, Simon
A1  - Badalyan, Artavazd
A1  - Mutihac, Radu-Cristian
A1  - Paulus, Florian
A1  - Leimkühler, Silke
A1  - Wollenberger, Ursula
A1  - Haag, Rainer
T1  - Dendritic polyglycerol-poly(ethylene glycol)-based polymer networks for biosensing application
JF  - ACS applied materials & interfaces
N2  - This work describes the formation of a new dendritic polyglycerol-poly(ethylene glycol)-based 3D polymer network as a matrix for immobilization of the redox enzyme periplasmatic aldehyde oxidoreductase to create an electrochemical biosensor. The novel network is built directly on the gold surface, where it simultaneously stabilizes the enzyme for up to 4 days. The prepared biosensors can be used for amperometric detection of benzaldehyde in the range of 0.8-400 mu M.
KW  - biosensors
KW  - hydrogel
KW  - amperometry
KW  - dendritic
Y1  - 2014
U6  - https://doi.org/10.1021/am502018x
SN  - 1944-8244
VL  - 6
IS  - 12
SP  - 8937
EP  - 8941
PB  - American Chemical Society
CY  - Washington
ER  - 
TY  - JOUR
A1  - Hönzke, Stefan
A1  - Gerecke, Christian
A1  - Elpelt, Anja
A1  - Zhang, Nan
A1  - Unbehauen, Michael
A1  - Kral, Vivian
A1  - Fleige, Emanuel
A1  - Paulus, Florian
A1  - Haag, Rainer
A1  - Schäfer-Korting, Monika
A1  - Kleuser, Burkhard
A1  - Hedtrich, Sarah
T1  - Tailored dendritic core-multishell nanocarriers for efficient dermal drug delivery: A systematic top-down approach from synthesis to preclinical testing
JF  - Journal of controlled release
N2  - Drug loaded dendritic core-multishell (CMS) nanocarriers are of especial interest for the treatment of skin diseases, owing to their striking dermal delivery efficiencies following topical applications. CMS nanocarriers are composed of a polyglycerol core, connected by amide-bonds to an inner alkyl shell and an outer methoxy poly(ethylene glycol) shell. Since topically applied nanocarriers are subjected to biodegradation, the application of conventional amide-based CMS nanocarriers (10-A-18-350) has been limited by the potential production of toxic polyglycerol amines. To circumvent this issue, three tailored ester-based CMS nanocarriers (10-E-12-350, 10-E-15-350, 10-E-18-350) of varying inner alkyl chain length were synthesized and comprehensively characterized in terms of particle size, drug loading, biodegradation and dermal drug delivery efficiency. Dexamethasone (DXM), a potent drug widely used for the treatment of inflammatory skin diseases, was chosen as a therapeutically relevant test compound for the present study. Ester-and amide-based CMS nanocarriers delivered DXM more efficiently into human skin than a commercially available DXM cream. Subsequent in vitro and in vivo toxicity studies identified CMS (10-E-15-350) as the most biocompatible carrier system. The anti-inflammatory potency of DXM-loaded CMS (10-E-15-350) nanocarriers was assessed in TNF alpha supplemented skin models, where a significant reduction of the pro-inflammatory cytokine IL-8 was seen, with markedly greater efficacy than commercial DXM cream. In summary, we report the rational design and characterization of tailored, biodegradable, ester-based CMS nanocarriers, and their subsequent stepwise screening for biocompatibility, dermal delivery efficiency and therapeutic efficacy in a top-down approach yielding the best carrier system for topical applications. (C) 2016 Elsevier B.V. All rights reserved.
KW  - Dendritic core-multishell nanocarriers
KW  - Biocompatibility
KW  - Dexamethasone
KW  - Inflammatory skin disease
KW  - Dermal drug delivery
KW  - Skin model
Y1  - 2016
U6  - https://doi.org/10.1016/j.jconrel.2016.06.030
SN  - 0168-3659
SN  - 1873-4995
VL  - 242
SP  - 50
EP  - 63
PB  - Elsevier
CY  - Amsterdam
ER  -