TY - JOUR A1 - Tejos, Ricardo A1 - Rodriguez-Furlan, Cecilia A1 - Adamowski, Maciej A1 - Sauer, Michael A1 - Norambuena, Lorena A1 - Friml, Jiri T1 - PATELLINS are regulators of auxin-mediated PIN1 relocation and plant development in Arabidopsis thaliana JF - Journal of cell science N2 - Coordinated cell polarization in developing tissues is a recurrent theme in multicellular organisms. In plants, a directional distribution of the plant hormone auxin is at the core of many developmental programs. A feedback regulation of auxin on the polarized localization of PIN auxin transporters in individual cells has been proposed as a self-organizing mechanism for coordinated tissue polarization, but the molecular mechanisms linking auxin signalling to PIN-dependent auxin transport remain unknown. We used a microarray-based approach to find regulators of the auxin-induced PIN relocation in Arabidopsis thaliana root, and identified a subset of a family of phosphatidylinositol transfer proteins (PITPs), the PATELLINs (PATLs). Here, we show that PATLs are expressed in partially overlapping cell types in different tissues going through mitosis or initiating differentiation programs. PATLs are plasma membrane-associated proteins accumulated in Arabidopsis embryos, primary roots, lateral root primordia and developing stomata. Higher order patl mutants display reduced PIN1 repolarization in response to auxin, shorter root apical meristem, and drastic defects in embryo and seedling development. This suggests that PATLs play a redundant and crucial role in polarity and patterning in Arabidopsis. KW - PATELLIN KW - Auxin KW - Arabidopsis thaliana KW - Auxin transport KW - Canalization Y1 - 2018 U6 - https://doi.org/10.1242/jcs.204198 SN - 0021-9533 SN - 1477-9137 VL - 131 IS - 2 PB - Company of Biologists Limited CY - Cambridge ER - TY - JOUR A1 - Melnick, Daniel A1 - Cisternas, Marco A1 - Moreno, Marcos A1 - Norambuena, Ricardo T1 - Estimating coseismic coastal uplift with an intertidal mussel calibration for the 2010 Maule Chile earthquake (M-w=8.8) JF - Quaternary science reviews : the international multidisciplinary research and review journal N2 - Coseismic coastal uplift has been quantified using sessile intertidal organisms after several great earthquakes following FitzRoy's pioneer measurements in 1835. A dense survey of such markers may complement space geodetic data to obtain an accurate distribution of fault slip and earthquake segmentation. However, uplift estimates based on diverse intertidal organisms tend to differ, because of few methodological and comparative studies. Here, we calibrate and estimate coastal uplift in the southern segment of the 2010 Maule, Chile earthquake (M-w = 8.8) using > 1100 post-earthquake elevation measurements of the sessile mussel Perumytilus purpuratus. This mussel is the predominant competitor for rocky shores all along the Pacific coast of South America, where it forms fringes or belts distinctively in the middle intertidal zone. These belts are centered at mean sea level and their width should equal one third of the tidal range. We measured belt widths close to this value at 40% of the sites, but overall widths are highly variable due to the unevenness in belt tops; belt bases, in turn, are rather regular. Belt top unevenness apparently results from locally-enhanced wave splash, whereas belt base evenness is controlled by predation. According to our measurements made beyond the earthquake rupture, the belt base is at the bottom of the middle intertidal zone, and thus we propose to estimate coastal uplift using the belt base mean elevation plus one sixth of the tidal range to reach mean sea level. Within errors our estimates agree with GPS displacements but differ from other methods. Comparisons of joint inversions for megathrust slip suggest combining space geodetic data with estimates from intertidal organisms may locally increase the detail of slip distributions. KW - Coastal uplift KW - Maule earthquake KW - Chile KW - Intertidal organisms KW - Perumytilus mussels KW - Slip distribution Y1 - 2012 U6 - https://doi.org/10.1016/j.quascirev.2012.03.012 SN - 0277-3791 VL - 42 IS - 5 SP - 29 EP - 42 PB - Elsevier CY - Oxford ER -