TY  - JOUR
A1  - Niehoff, Ann-Christin
A1  - Bauer, Oliver Bolle
A1  - Kröger, Sabrina
A1  - Fingerhut, Stefanie
A1  - Schulz, Jacqueline
A1  - Meyer, Sören
A1  - Sperling, Michael
A1  - Jeibmann, Astrid
A1  - Schwerdtle, Tanja
A1  - Karst, Uwe
T1  - Quantitative Bioimaging to Investigate the Uptake of Mercury Species in Drosophila melanogaster
JF  - Analytical chemistry
N2  - The uptake of mercury species in the model organism Drosophila melanogaster was investigated by elemental bioimaging using laser ablation-inductively coupled plasma mass spectrometry (LA-ICPMS). The mercury distribution in Drosophila melanogaster was analyzed for the three species mercury(II) chloride, methylmercury chloride, and thimerosal after intoxication. A respective analytical method was developed and applied to the analysis of the entire Drosophila melanogaster first, before a particular focus was directed to the cerebral areas of larvae and adult flies. For quantification of mercury, matrix-matched standards based on gelatin were prepared. Challenges of spatially dissolved mercury determination, namely, strong evaporation issues of the analytes and an inhomogeneous distribution of mercury in the standards due to interactions with cysteine containing proteins of the gelatin were successfully addressed by complexation with meso-2,3-dimercaptosuccinic acid (DMSA). No mercury was detected in the cerebral region for mercury(II) chloride, whereas both organic species showed the ability to cross the blood brain barrier. Quantitatively, the mercury level in the brain exceeded the fed concentration indicating mercury enrichment, which was approximately 3 times higher for methylmercury chloride than for thimerosal.
Y1  - 2015
U6  - https://doi.org/10.1021/acs.analchem.5b02500
SN  - 0003-2700
SN  - 1520-6882
VL  - 87
IS  - 20
SP  - 10392
EP  - 10396
PB  - American Chemical Society
CY  - Washington
ER  - 
TY  - JOUR
A1  - Niehoff, Ann-Christin
A1  - Schulz, Jacqueline
A1  - Soltwisch, Jens
A1  - Meyer, Soren
A1  - Kettling, Hans
A1  - Sperling, Michael
A1  - Jeibmann, Astrid
A1  - Dreisewerd, Klaus
A1  - Francesconi, Kevin A.
A1  - Schwerdtle, Tanja
A1  - Karst, Uwe
T1  - Imaging by Elemental and Molecular Mass Spectrometry Reveals the Uptake of an Arsenolipid in the Brain of Drosophila melanogaster
JF  - Analytical chemistry
N2  - Arsenic-containing lipids (arsenolipids) are natural products of marine organisms such as fish, invertebrates, and algae, many of which are important seafoods. A major group of arsenolipids, namely, the arsenic-containing hydrocarbons (AsHC), have recently been shown to be cytotoxic to human liver and bladder cells, a result that has stimulated interest in the chemistry and toxicology of these compounds. In this study, elemental laser ablation-inductively coupled plasma mass spectrometry (LA-ICPMS) and molecular matrix-assisted laser desorption/ionization (MALDI-)MS were used to image and quantify the uptake of an AsHC in the model organism Drosophila melanogaster. Using these two complementary methods, both an enrichment of arsenic and the presence of the AsHC in the brain were revealed, indicating that the intact arsenolipid had crossed the blood-brain barrier. Simultaneous acquisition of quantitative elemental concentrations and molecular distributions could allow new insight into organ-specific enrichment and possible transportation processes of arsenic-containing bioactive compounds in living organisms.
Y1  - 2016
U6  - https://doi.org/10.1021/acs.analchem.6b00333
SN  - 0003-2700
SN  - 1520-6882
VL  - 88
SP  - 5258
EP  - 5263
PB  - American Chemical Society
CY  - Washington
ER  -