TY - JOUR A1 - Ghylin, Trevor W. A1 - Garcia, Sarahi L. A1 - Moya, Francisco A1 - Oyserman, Ben O. A1 - Schwientek, Patrick A1 - Forest, Katrina T. A1 - Mutschler, James A1 - Dwulit-Smith, Jeffrey A1 - Chan, Leong-Keat A1 - Martinez-Garcia, Manuel A1 - Sczyrba, Alexander A1 - Stepanauskas, Ramunas A1 - Grossart, Hans-Peter A1 - Woyke, Tanja A1 - Warnecke, Falk A1 - Malmstrom, Rex A1 - Bertilsson, Stefan A1 - McMahon, Katherine D. T1 - Comparative single-cell genomics reveals potential ecological niches for the freshwater acl Actinobacteria lineage JF - The ISME journal : multidisciplinary journal of microbial ecology N2 - Members of the acI lineage of Actinobacteria are the most abundant microorganisms in most freshwater lakes; however, our understanding of the keys to their success and their role in carbon and nutrient cycling in freshwater systems has been hampered by the lack of pure cultures and genomes. We obtained draft genome assemblies from 11 single cells representing three acI tribes (acI-A1, acI-A7, acI-B1) from four temperate lakes in the United States and Europe. Comparative analysis of acI SAGs and other available freshwater bacterial genomes showed that acI has more gene content directed toward carbohydrate acquisition as compared to Polynucleobacter and LD12 Alphaproteobacteria, which seem to specialize more on carboxylic acids. The acI genomes contain actinorhodopsin as well as some genes involved in anaplerotic carbon fixation indicating the capacity to supplement their known heterotrophic lifestyle. Genome-level differences between the acI-A and acI-B clades suggest specialization at the clade level for carbon substrate acquisition. Overall, the acI genomes appear to be highly streamlined versions of Actinobacteria that include some genes allowing it to take advantage of sunlight and N-rich organic compounds such as polyamines, di-and oligopeptides, branched-chain amino acids and cyanophycin. This work significantly expands the known metabolic potential of the cosmopolitan freshwater acI lineage and its ecological and genetic traits. Y1 - 2014 U6 - https://doi.org/10.1038/ismej.2014.135 SN - 1751-7362 SN - 1751-7370 VL - 8 IS - 12 SP - 2503 EP - 2516 PB - Nature Publ. Group CY - London ER - TY - JOUR A1 - Fernandez-Fernandez, Jaime A1 - Moya-Ramon, Manuel A1 - Santos-Rosa, Francisco Javier A1 - Gantois, Petrus A1 - Nakamura, Fabio Yuzo A1 - Sanz-Rivas, David A1 - Granacher, Urs T1 - Within-session sequence of the tennis serve training in youth elite players JF - International journal of environmental research and public health N2 - The influence of muscular fatigue on tennis serve performance within regular training sessions is unclear. Therefore, the aim of the present study was to examine the within-session sequence of the tennis serve in youth tennis. Twenty-five young male (14.9 +/- 0.9 years) and female (14.5 +/- 0.9 years) players participated in this within-subject crossover study, and they were randomly but sex-matched assigned to different training sequences (serve exercise before tennis training (BTS) or after tennis training (ATS)). Pre- and post-tests included serve velocity performance and accuracy, shoulder strength, and range-of-motion (ROM) performance (internal/external rotation). Results showed that after one week of serve training conducted following the ATS sequence, significant decreases were found in serve performance (e.g., speed and accuracy), with standardized differences ranging from d = 0.29 to 1.13, as well as the shoulder function (strength [d = 0.20 to 1.0] and ROM [d = 0.17 to 0.31]) in both female and male players, compared to the BTS sequence. Based on the present findings, it appears more effective to implement serve training before the regular tennis training in youth players. If applied after training, excessive levels of fatigue may cause shoulder imbalances that could be related to an increased injury risk. KW - athletes KW - athletic performance KW - fatigue KW - fitness KW - shoulder strength KW - range of motion Y1 - 2020 U6 - https://doi.org/10.3390/ijerph18010244 SN - 1660-4601 VL - 18 IS - 1 PB - MDPI CY - Basel ER -