TY - JOUR A1 - Witzel, Katja A1 - Strehmel, Nadine A1 - Baldermann, Susanne A1 - Neugart, Susanne A1 - Becker, Yvonne A1 - Becker, Matthias A1 - Berger, Beatrice A1 - Scheel, Dierk A1 - Grosch, Rita A1 - Schreiner, Monika A1 - Ruppel, Silke T1 - Arabidopsis thaliana root and root exudate metabolism is altered by the growth-promoting bacterium Kosakonia radicincitans DSM 16656(T) JF - Plant and soil N2 - Plant growth-promoting bacteria (PGPB) affect host physiological processes in various ways. This study aims at elucidating the dependence of bacterial-induced growth promotion on the plant genotype and characterizing plant metabolic adaptations to PGPB. Eighteen Arabidopsis thaliana accessions were inoculated with the PGPB strain Kosakonia radicincitans DSM 16656(T). Colonisation pattern was assessed by enhanced green fluorescent protein (eGFP)-tagged K. radicincitans in three A. thaliana accessions differing in their growth response. Metabolic impact of bacterial colonisation was determined for the best responding accession by profiling distinct classes of plant secondary metabolites and root exudates. Inoculation of 18 A. thaliana accessions resulted in a wide range of growth responses, from repression to enhancement. Testing the bacterial colonisation of three accessions did not reveal a differential pattern. Profiling of plant secondary metabolites showed a differential accumulation of glucosinolates, phenylpropanoids and carotenoids in roots. Analysis of root exudates demonstrated that primary and secondary metabolites were predominantly differentially depleted by bacterial inoculation. The plant genotype controls the bacterial growth promoting traits. Levels of lutein and beta-carotene were elevated in inoculated roots. Supplementing a bacterial suspension with beta-carotene increased bacterial growth, while this was not the case when lutein was applied, indicating that beta-carotene could be a positive regulator of plant growth promotion. KW - Arabidopsis KW - Carotenoids KW - Glucosinolates KW - Plant growth promoting bacteria KW - Phenylpropanoids KW - Root exudates Y1 - 2017 U6 - https://doi.org/10.1007/s11104-017-3371-1 SN - 0032-079X SN - 1573-5036 VL - 419 SP - 557 EP - 573 PB - Springer CY - Dordrecht ER - TY - JOUR A1 - Meiners, Jana A1 - Palmieri, Vittoria A1 - Klopfleisch, Robert A1 - Ebel, Jana-Fabienne A1 - Japtok, Lukasz A1 - Schumacher, Fabian A1 - Yusuf, Ayan Mohamud A1 - Becker, Katrin Anne A1 - Zöller, Julia A1 - Hose, Matthias A1 - Kleuser, Burkhard A1 - Hermann, Dirk Matthias A1 - Kolesnick, Richard N. A1 - Buer, Jan A1 - Hansen, Wiebke A1 - Westendorf, Astrid M. T1 - Intestinal acid sphingomyelinase protects from severe Pathogen-Driven Colitis JF - Frontiers in immunology N2 - Inflammatory diseases of the gastrointestinal tract are emerging as a global problem with increased evidence and prevalence in numerous countries. A dysregulated sphingolipid metabolism occurs in patients with ulcerative colitis and is discussed to contribute to its pathogenesis. In the present study, we determined the impact of acid sphingomyelinase (Asm), which catalyzes the hydrolysis of sphingomyelin to ceramide, on the course of Citrobacter (C.) rodentium-driven colitis. C. rodentium is an enteric pathogen and induces colonic inflammation very similar to the pathology in patients with ulcerative colitis. We found that mice with Asm deficiency or Asm inhibition were strongly susceptible to C. rodentium infection. These mice showed increased levels of C. rodentium in the feces and were prone to bacterial spreading to the systemic organs. In addition, mice lacking Asm activity showed an uncontrolled inflammatory T(h)1 and T(h)17 response, which was accompanied by a stronger colonic pathology compared to infected wild type mice. These findings identified Asm as an essential regulator of mucosal immunity to the enteric pathogen C. rodentium. KW - Citrobacter rodentium KW - colitis KW - acid sphingomyelinase KW - amitriptyline KW - T(h)1 KW - T(h)17 Y1 - 2019 U6 - https://doi.org/10.3389/fimmu.2019.01386 SN - 1664-3224 VL - 10 PB - Frontiers Research Foundation CY - Lausanne ER - TY - BOOK A1 - Becker, Matthias A1 - Kümmel, Egbert A1 - Ott, Andreas T1 - Wohnungseigentum : Grundlagen, Systematik, Praxis Y1 - 2003 SN - 3-504-45026-5 PB - Schmidt CY - Köln ER - TY - JOUR A1 - Becker, Matthias T1 - Grundrechtsschutz durch Zivilgerichte Y1 - 2003 ER - TY - JOUR A1 - Becker, Matthias T1 - Die Anfechtung des Abberufungsbeschlusses durch den abberufenen Verwalter : Besprechung von OLG Hamm, Beschluss vom 27.11.2001 - 15 W 326/01 Y1 - 2002 ER - TY - JOUR A1 - Becker, Matthias T1 - Die Haftung der Anlagegesellschafter geschlossener Immobilienfonds als Gesellschaft bürgerlichen Rechts : Besprechung von BGH, Urteil vom 21.1.2002 - II ZR 2/00 Y1 - 2002 ER - TY - JOUR A1 - Becker, Matthias T1 - Beschlusskompetenz kraft Vereinbarung : sog. Öffnungsklausel Y1 - 2002 ER - TY - JOUR A1 - Becker, Matthias T1 - Irrtümliche Falschbezeichnung des Sondereigentums bei der Veräußerung von Teileigentum; Beitragspflicht des Scheineigentümers : Besprechung von BayObLG, Beschluss vom 19.9.2001 - 2Z BR 101/01 Y1 - 2002 ER - TY - JOUR A1 - Becker, Matthias T1 - Die Geltendmachung von Ersatzansprüchen gegen den Frachtführer gemäß § 421 Abs. 1 Satz 2 HGB Y1 - 2002 ER - TY - JOUR A1 - Becker, Matthias T1 - Ergebnisfeststellung und Beschlusstatbestand Y1 - 2002 ER -