TY - JOUR A1 - Villatoro, José Andrés A1 - Weber, M. A1 - Zühlke, Martin A1 - Lehmann, A. A1 - Zenichowski, Karl A1 - Riebe, Daniel A1 - Beitz, Toralf A1 - Löhmannsröben, Hans-Gerd A1 - Kreuzer, O. T1 - Structural characterization of synthetic peptides using electrospray ion mobility spectrometry and molecular dynamics simulations JF - International Journal of Mass Spectrometry N2 - Electrospray ionization-ion mobility spectrometry was employed for the determination of collision cross sections (CCS) of 25 synthetically produced peptides in the mass range between 540-3310 Da. The experimental measurement of the CCS is complemented by their calculation applying two different methods. One prediction method is the intrinsic size parameter (ISP) method developed by the Clemmer group. The second new method is based on the evaluation of molecular dynamics (MD) simulation trajectories as a whole, resulting in a single, averaged collision cross-section value for a given peptide in the gas phase. A high temperature MD simulation is run in order to scan through the whole conformational space. The lower temperature conformational distribution is obtained through thermodynamic reweighting. In the first part, various correlations, e.g. CCS vs. mass and inverse mobility vs. m/z correlations, are presented. Differences in CCS between peptides are also discussed in terms of their respective mass and m/z differences, as well as their respective structures. In the second part, measured and calculated CCS are compared. The agreement between the prediction results and the experimental values is in the same range for both calculation methods. While the calculation effort of the ISP method is much lower, the MD method comprises several tools providing deeper insights into the conformations of peptides. Advantages and limitations of both methods are discussed. Based on the separation of two pairs of linear and cyclic peptides of virtually the same mass, the influence of the structure on the cross sections is discussed. The shift in cross section differences and peak shape after transition from the linear to the cyclic peptide can be well understood by applying different MD tools, e.g. the root-mean-square deviation (RMSD) and the root mean square fluctuation (RMSF). (C) 2018 Elsevier B.V. All rights reserved. KW - Ion mobility spectrometry KW - Electrospray ionization KW - Peptides KW - Collision cross-section KW - Molecular dynamics Y1 - 2019 U6 - https://doi.org/10.1016/j.ijms.2018.10.036 SN - 1387-3806 SN - 1873-2798 VL - 436 SP - 108 EP - 117 PB - Elsevier CY - Amsterdam ER - TY - BOOK A1 - Pohlenz, Philipp A1 - Schubarth, Wilfried A1 - Speck, Karsten A1 - Seidel, Andreas A1 - Winter, Martin A1 - Heine, Christoph A1 - Kleinfeld, Merle A1 - Sarrar, Lea A1 - Gemsa, Charlotte A1 - Wendland, Mirko A1 - Schlumm, Katharina A1 - Lehmann, Uta A1 - Kummerow, Udo A1 - Bastian, Laura A1 - Kamm, Caroline A1 - Niproschke, Saskia A1 - Sochadse, Lascha A1 - Kotzur, Katharina A1 - Hebert, Sebastian A1 - Voigt, Frank A1 - Kopp, Andrea ED - Schubarth, Wilfried ED - Speck, Karsten ED - Seidel, Andreas T1 - Nach Bologna: Praktika im Studium – Pflicht oder Kür? T1 - Beyond Bologna: internship in studies – obligation or option? BT - Empirische Analysen und Empfehlungen für die Hochschulpraxis BT - Empirical analysis and recommendations on the praxis of higher education N2 - Mit dem vorliegenden Band „Nach Bologna: Praktika im Studium – Pflicht oder Kür? Empirische Analysen und Empfehlungen für die Hochschulpraxis“ von Wilfried Schubarth, Karsten Speck und Andreas Seidel wird die Reihe „Potsdamer Beiträge zur Lehrevaluation“ unter neuem Titel und veränderter inhaltlicher Schwerpunktsetzung fortgeführt. Die Umbenennung in „Potsdamer Beiträge zur Hochschulforschung“ versteht sich als ein Schritt hin zu einer thematischen Öffnung der Reihe für die verschiedensten Felder der Hochschulforschung. Der vorliegende Band widmet sich einem der zentralen Reformziele von Bologna: der Frage des Praxis- und Berufsbezugs und dabei insbesondere den Praxisphasen im Studium. Mit der Bologna-Reform werden im bildungspolitischen Bereich sehr vielfältige strukturelle und inhaltliche Ziele verfolgt. Das Ziel dieses Bandes besteht deshalb darin, empirische Forschungen zu Praxisbezügen und Praxisphasen im Studium vorzustellen, diese in den Kontext aktueller Debatten um Studienqualität und Studienreform zu stellen sowie Folgerungen für die Gestaltung von Praxisphasen abzuleiten. Inhaltliche Schwerpunkte bilden das BMBF-Forschungsprojekt ProPrax und die Praxisphasen im Lehramtsstudium. Die Beiträge dieses Bandes gehen aus einem gleichnamigen Workshop hervor, der am 1. Oktober 2010 in Potsdam stattfand. N2 - The present conference volume „Beyond Bologna: Internship in studies – obligation or option? Empirical analysis and recommendations on the praxis of higher education“ by Wilfried Schubarth, Karsten Speck and Andreas Seidel is part of the serial “Potsdamer Beiträge zur Lehrevaluation” which is actually renamed as “Potsdamer Beiträge zur Hochschulforschung“. The new title supports modified focal points for research and provides opportunities for several fields of higher education research. The volume focuses on the practical relevance and the development of professional skills in academic studies as one of the central intentions of the Bologna Process. This Reform involves a variety of structural and content-related intentions in the field of educational policy. The aim of this conference volume is therefore to present empirical research with regard to practical relevance and internship in academic studies. It is linked to the current debate concerning quality of academic studies as well as the higher education reform in general and draws conclusions for the practical implementation of academic internships. The ProPrax research project, founded by the BMBF, and its first results as well as internships in teacher education are main issues of this volume. The contributions result from the correspondent Potsdam conference on October 1st 2010. T3 - Potsdamer Beiträge zur Hochschulforschung - 1 KW - Bologna-Prozess KW - Hochschulforschung KW - Lehrerbildung KW - Praxisphasen KW - Kompetenzentwicklung KW - Studienqualität KW - Bologna Process KW - higher education research KW - teacher education KW - internships KW - competence development KW - quality of academic studies Y1 - 2011 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-51033 SN - 978-3-86956-123-3 PB - Universitätsverlag Potsdam CY - Potsdam ER - TY - THES A1 - Lehmann, Martin T1 - Back to the roots : regulation of arabidopsis root metabolism during oxidative stress Y1 - 2011 CY - Potsdam ER - TY - JOUR A1 - Janssen, Annette B. G. A1 - Arhonditsis, George B. A1 - Beusen, Arthur A1 - Bolding, Karsten A1 - Bruce, Louise A1 - Bruggeman, Jorn A1 - Couture, Raoul-Marie A1 - Downing, Andrea S. A1 - Elliott, J. Alex A1 - Frassl, Marieke A. A1 - Gal, Gideon A1 - Gerla, Daan J. A1 - Hipsey, Matthew R. A1 - Hu, Fenjuan A1 - Ives, Stephen C. A1 - Janse, Jan H. A1 - Jeppesen, Erik A1 - Joehnk, Klaus D. A1 - Kneis, David A1 - Kong, Xiangzhen A1 - Kuiper, Jan J. A1 - Lehmann, Moritz K. A1 - Lemmen, Carsten A1 - Oezkundakci, Deniz A1 - Petzoldt, Thomas A1 - Rinke, Karsten A1 - Robson, Barbara J. A1 - Sachse, Rene A1 - Schep, Sebastiaan A. A1 - Schmid, Martin A1 - Scholten, Huub A1 - Teurlincx, Sven A1 - Trolle, Dennis A1 - Troost, Tineke A. A1 - Van Dam, Anne A. A1 - Van Gerven, Luuk P. A. A1 - Weijerman, Mariska A1 - Wells, Scott A. A1 - Mooij, Wolf M. T1 - Exploring, exploiting and evolving diversity of aquatic ecosystem models: a community perspective JF - Aquatic ecology : the international forum covering research in freshwater and marine environments N2 - Here, we present a community perspective on how to explore, exploit and evolve the diversity in aquatic ecosystem models. These models play an important role in understanding the functioning of aquatic ecosystems, filling in observation gaps and developing effective strategies for water quality management. In this spirit, numerous models have been developed since the 1970s. We set off to explore model diversity by making an inventory among 42 aquatic ecosystem modellers, by categorizing the resulting set of models and by analysing them for diversity. We then focus on how to exploit model diversity by comparing and combining different aspects of existing models. Finally, we discuss how model diversity came about in the past and could evolve in the future. Throughout our study, we use analogies from biodiversity research to analyse and interpret model diversity. We recommend to make models publicly available through open-source policies, to standardize documentation and technical implementation of models, and to compare models through ensemble modelling and interdisciplinary approaches. We end with our perspective on how the field of aquatic ecosystem modelling might develop in the next 5-10 years. To strive for clarity and to improve readability for non-modellers, we include a glossary. KW - Water quality KW - Ecology KW - Geochemistry KW - Hydrology KW - Hydraulics KW - Hydrodynamics KW - Physical environment KW - Socio-economics KW - Model availability KW - Standardization KW - Linking Y1 - 2015 U6 - https://doi.org/10.1007/s10452-015-9544-1 SN - 1386-2588 SN - 1573-5125 VL - 49 IS - 4 SP - 513 EP - 548 PB - Springer CY - Dordrecht ER - TY - JOUR A1 - Franz, Alexandra A1 - Többens, Daniel M. A1 - Lehmann, Frederike A1 - Kärgell, Martin A1 - Schorr, Susan T1 - The influence of deuteration on the crystal structure of hybrid halide perovskites: a temperature-dependent neutron diffraction study of FAPbBr(3) JF - Acta crystallographica; Section B, Structural science, crystal engineering and materials N2 - This paper discusses the full structural solution of the hybrid perovskite formamidinium lead tribromide (FAPbBr(3)) and its temperature-dependent phase transitions in the range from 3 K to 300 K using neutron powder diffraction and synchrotron X-ray diffraction. Special emphasis is put on the influence of deuteration on formamidinium, its position in the unit cell and disordering in comparison to fully hydrogenated FAPbBr(3). The temperature-dependent measurements show that deuteration critically influences the crystal structures, i.e. results in partially-ordered temperature-dependent structural modifications in which two symmetry-independent molecule positions with additional dislocation of the molecular centre atom and molecular angle inclinations are present. KW - hybrid perovskite KW - FAPbBr(3) KW - deuteration KW - neutron powder diffraction KW - crystal structure Y1 - 2020 U6 - https://doi.org/10.1107/S2052520620002620 SN - 2052-5206 VL - 76 IS - 2 SP - 267 EP - 274 PB - Wiley-Blackwell CY - Oxford [u.a.] ER - TY - JOUR A1 - Hermanussen, Michael A1 - Lieberman, L. S. A1 - Schönfeld Janewa, U. A1 - Scheffler, Christiane A1 - Ghosh, A. A1 - Bogin, Barry A1 - Godina, E. A1 - Kaczmarek, M. A1 - El-Shabrawi, M. A1 - Salama, E. E. A1 - Rühli, F. J. A1 - Staub, K. A1 - Woitek, U. A1 - Blaha, Pawel A1 - vanBuurren, S. A1 - Lehmann, A. A1 - Satake, T. A1 - Thodberg, H. H. A1 - Jopp, E. A1 - Kirchengast, S. A1 - Tutkuviene, J. A1 - McIntyre, M. H. A1 - Wittwer-Backofen, U. A1 - Boldsen, J. L. A1 - Martin, D. D. A1 - Meier, J. T1 - Diversity in auxology: between theory and practice Proceedings of the 18th Aschauer Soiree, 13th November 2010 Y1 - 2012 SN - 0003-5548 ER - TY - JOUR A1 - Hermanussen, Michael A1 - Lieberman, Leslie Su A1 - Janewa, V. Schoenfeld A1 - Scheffler, Christiane A1 - Ghosh, Arunava A1 - Bogin, Barry A1 - Godina, Elena A1 - Kaczmarek, M. A1 - El-Shabrawi, M. A1 - Salama, E. E. A1 - Rühli, Frank J. A1 - Staub, Kaspar A1 - Woitek, U. A1 - Blaha, Pawel A1 - Aßmann, Christian A1 - van Buuren, Stef A1 - Lehmann, A. A1 - Satake, T. A1 - Thodberg, H. H. A1 - Jopp, E. A1 - Kirchengast, S. A1 - Tutkuviene, J. A1 - McIntyre, M. H. A1 - Wittwer-Backofen, U. A1 - Boldsen, Jesper L. A1 - Martin, D. D. A1 - Meier, J. T1 - Diversity in auxology between theory and practice JF - Journal of biological and clinical anthropology : Anthropologischer Anzeiger ; Mitteilungsorgan der Gesellschaft für Anthropologie N2 - Auxology has developed from mere describing child and adolescent growth into a vivid and interdisciplinary research area encompassing human biologists, physicians, social scientists, economists and biostatisticians. The meeting illustrated the diversity in auxology, with the various social, medical, biological and biostatistical aspects in studies on child growth and development. KW - child growth KW - adolescent growth KW - child development KW - height KW - weight KW - body mass KW - socio-economic environment Y1 - 2012 U6 - https://doi.org/10.1127/0003-5548/2012/0133 SN - 0003-5548 VL - 69 IS - 2 SP - 159 EP - 174 PB - Schweizerbart CY - Stuttgart ER - TY - JOUR A1 - Hensch, Martin A1 - Dahm, Torsten A1 - Ritter, Joachim A1 - Heimann, Sebastian A1 - Schmidt, Bernd A1 - Stange, Stefan A1 - Lehmann, Klaus T1 - Deep low-frequency earthquakes reveal ongoing magmatic recharge beneath Laacher See Volcano (Eifel, Germany) JF - Geophysical journal international N2 - The occurrence of deep low-frequency (DLF) microearthquakes beneath volcanoes is commonly attributed to mass transport in the volcanic plumbing system and used to infer feeding channels from and into magma reservoirs. The key question is how magmas migrate from depth to the shallow crust and whether magma reservoirs are currently being recharged. For the first time since the improvement of the local seismic networks in the East Eifel region (Rhineland-Palatinate, Germany), we detect and locate recurrent DLF earthquakes in the lower crust and upper mantle beneath the Laacher See Volcano (LSV), using a joint data set of permanent sensors and a temporary deployment. So far, eight DLF earthquake sequences were observed in four distinct clusters between 10 and 40 km depth. These clusters of weak events (M-L< 2) align along an approximately 80. southeast dipping line south of the LSV. Moment tensor solutions of these events have large shear components, and the irregular dispersion and long coda of body waves indicate interaction processes between shear cracks and fluids. We find a rotation of P-axes orientation for shallow tectonic earthquakes compared to DLF events, indicating that the stress field in the depth interval of DLF events might favour a vertical migration of magma or magmatic fluids. The caldera of the LSV was formed by the last major eruption of the East Eifel Volcanic Field only 12.9 kyr ago, fed by a shallow magma chamber at 5-8 km depth and erupting a total magma volume of 6.7 km(3). The observed DLF earthquake activity and continuous volcanic gas emissions around the LSV indicate an active magmatic system, possibly connected with an upper mantle melt zone. KW - Waveform inversion KW - Volcano seismology KW - Magma migration and fragmentation KW - Volcano monitoring Y1 - 2019 U6 - https://doi.org/10.1093/gji/ggy532 SN - 0956-540X SN - 1365-246X VL - 216 IS - 3 SP - 2025 EP - 2036 PB - Oxford Univ. Press CY - Oxford ER - TY - JOUR A1 - Puchkov, Dmytro A1 - Müller, Paul Markus A1 - Lehmann, Martin A1 - Matthäus, Claudia T1 - Analyzing the cellular plasma membrane by fast and efficient correlative STED and platinum replica EM JF - Frontiers in cell and developmental biology N2 - The plasma membrane of mammalian cells links transmembrane receptors, various structural components, and membrane-binding proteins to subcellular processes, allowing inter- and intracellular communication. Therefore, membrane-binding proteins, together with structural components such as actin filaments, modulate the cell membrane in their flexibility, stiffness, and curvature. Investigating membrane components and curvature in cells remains challenging due to the diffraction limit in light microscopy. Preparation of 5–15-nm-thin plasma membrane sheets and subsequent inspection by metal replica transmission electron microscopy (TEM) reveal detailed information about the cellular membrane topology, including the structure and curvature. However, electron microscopy cannot identify proteins associated with specific plasma membrane domains. Here, we describe a novel adaptation of correlative super-resolution light microscopy and platinum replica TEM (CLEM-PREM), allowing the analysis of plasma membrane sheets with respect to their structural details, curvature, and associated protein composition. We suggest a number of shortcuts and troubleshooting solutions to contemporary PREM protocols. Thus, implementation of super-resolution stimulated emission depletion (STED) microscopy offers significant reduction in sample preparation time and reduced technical challenges for imaging and analysis. Additionally, highly technical challenges associated with replica preparation and transfer on a TEM grid can be overcome by scanning electron microscopy (SEM) imaging. The combination of STED microscopy and platinum replica SEM or TEM provides the highest spatial resolution of plasma membrane proteins and their underlying membrane and is, therefore, a suitable method to study cellular events like endocytosis, membrane trafficking, or membrane tension adaptations. KW - plasma membrane KW - endocytosis KW - CLEM KW - STED KW - TEM KW - SEM KW - electron microscopy Y1 - 2023 U6 - https://doi.org/10.3389/fcell.2023.1305680 SN - 2296-634X VL - 11 PB - Frontiers Media CY - Lausanne ER - TY - JOUR A1 - Lehmann, Nico A1 - Kuhn, Yves-Alain A1 - Keller, Martin A1 - Aye, Norman A1 - Herold, Fabian A1 - Draganski, Bogdan A1 - Taube, Wolfgang A1 - Taubert, Marco T1 - Brain activation during active balancing and its behavioral relevance in younger and older adults BT - a functional near-infrared spectroscopy (fNIRS) study JF - Frontiers in Aging Neuroscience N2 - Age-related deterioration of balance control is widely regarded as an important phenomenon influencing quality of life and longevity, such that a more comprehensive understanding of the neural mechanisms underlying this process is warranted. Specifically, previous studies have reported that older adults typically show higher neural activity during balancing as compared to younger counterparts, but the implications of this finding on balance performance remain largely unclear. Using functional near-infrared spectroscopy (fNIRS), differences in the cortical control of balance between healthy younger (n = 27) and older (n = 35) adults were explored. More specifically, the association between cortical functional activity and balance performance across and within age groups was investigated. To this end, we measured hemodynamic responses (i.e., changes in oxygenated and deoxygenated hemoglobin) while participants balanced on an unstable device. As criterion variables for brain-behavior-correlations, we also assessed postural sway while standing on a free-swinging platform and while balancing on wobble boards with different levels of difficulty. We found that older compared to younger participants had higher activity in prefrontal and lower activity in postcentral regions. Subsequent robust regression analyses revealed that lower prefrontal brain activity was related to improved balance performance across age groups, indicating that higher activity of the prefrontal cortex during balancing reflects neural inefficiency. We also present evidence supporting that age serves as a moderator in the relationship between brain activity and balance, i.e., cortical hemodynamics generally appears to be a more important predictor of balance performance in the older than in the younger. Strikingly, we found that age differences in balance performance are mediated by balancing-induced activation of the superior frontal gyrus, thus suggesting that differential activation of this region reflects a mechanism involved in the aging process of the neural control of balance. Our study suggests that differences in functional brain activity between age groups are not a mere by-product of aging, but instead of direct behavioral relevance for balance performance. Potential implications of these findings in terms of early detection of fall-prone individuals and intervention strategies targeting balance and healthy aging are discussed. KW - aging KW - neuroimaging KW - functional near-infrared spectroscopy (fNIRS) KW - balance KW - postural control KW - prefrontal cortex KW - neural inefficiency Y1 - 2022 U6 - https://doi.org/10.3389/fnagi.2022.828474 SN - 1663-4365 VL - 14 PB - Frontiers Media CY - Lausanne ER - TY - JOUR A1 - Koziel, Slawomir A1 - Scheffler, Christiane A1 - Tutkuviene, Janina A1 - Jakimaviciene, Egle Marija A1 - Mumm, Rebekka A1 - Barbieri, Davide A1 - Godina, Elena A1 - El-Shabrawi, Mortada A1 - Elhusseini, Mona A1 - Musalek, Martin A1 - Pruszkowska-Przybylska, Paulina A1 - El Dash, Hanaa H. A1 - Safar, Hebatalla Hassan A1 - Lehmann, Andreas A1 - Swanson, James A1 - Bogin, Barry A1 - Liu, Yuk-Chien A1 - Groth, Detlef A1 - Kirchengast, Sylvia A1 - Siniarska, Anna A1 - Nieczuja-Dwojacka, Joanna A1 - Kralik, Miroslav A1 - Satake, Takashi A1 - Harc, Tomasz A1 - Roelants, Mathieu A1 - Hermanussen, Michael T1 - Meeting Report: Growth and social environment BT - Proceedings of the 25th Aschauer Soiree, held at Krobielowice, Poland, November 18th 2017 JF - Pediatric Endocrinology Reviews N2 - Twenty-two scientists met at Krobielowice, Poland, to discuss the impact of the social environment, spatial proximity, migration, poverty, but also psychological factors such as body perception and satisfaction, and social stressors such as elite sports, and teenage pregnancies, on child and adolescent growth. The data analysis included linear mixed effects models with different random effects, Monte Carlo analyses, and network simulations. The work stressed the importance of the peer group, but also included historic material, some considerations about body proportions, and growth in chronic liver, and congenital heart disease. KW - Body height KW - Social environment KW - Strategic growth adjustment KW - Competitive growth KW - Community effects on growth Y1 - 2018 U6 - https://doi.org/10.17458/per.vol15.2018.ksh.mr.GrowthSocialEnvironment SN - 1565-4753 VL - 15 IS - 4 SP - 319 EP - 329 PB - Medical Media CY - Netanya ER -