TY  - JOUR
A1  - Jüppner, Jessica
A1  - Mubeen, Umarah
A1  - Leisse, Andrea
A1  - Caldana, Camila
A1  - Brust, Henrike
A1  - Steup, Martin
A1  - Herrmann, Marion
A1  - Steinhauser, Dirk
A1  - Giavalisco, Patrick
T1  - Dynamics of lipids and metabolites during the cell cycle of Chlamydomonas reinhardtii
JF  - The plant journal
N2  - Metabolites and lipids are the final products of enzymatic processes, distinguishing the different cellular functions and activities of single cells or whole tissues. Understanding these cellular functions within a well-established model system requires a systemic collection of molecular and physiological information. In the current report, the green alga Chlamydomonas reinhardtii was selected to establish a comprehensive workflow for the detailed multi-omics analysis of a synchronously growing cell culture system. After implementation and benchmarking of the synchronous cell culture, a two-phase extraction method was adopted for the analysis of proteins, lipids, metabolites and starch from a single sample aliquot of as little as 10-15million Chlamydomonas cells. In a proof of concept study, primary metabolites and lipids were sampled throughout the diurnal cell cycle. The results of these time-resolved measurements showed that single compounds were not only coordinated with each other in different pathways, but that these complex metabolic signatures have the potential to be used as biomarkers of various cellular processes. Taken together, the developed workflow, including the synchronized growth of the photoautotrophic cell culture, in combination with comprehensive extraction methods and detailed metabolic phenotyping has the potential for use in in-depth analysis of complex cellular processes, providing essential information for the understanding of complex biological systems.
KW  - Chlamydomonas reinhardtii
KW  - synchronized cell cultures
KW  - photoautotrophic growth
KW  - cell cycle
KW  - metabolomics
KW  - lipidomics
KW  - systems biology
KW  - two-phase extraction
KW  - diurnal cycle
KW  - technical advance
Y1  - 2017
U6  - https://doi.org/10.1111/tpj.13642
SN  - 0960-7412
SN  - 1365-313X
VL  - 92
SP  - 331
EP  - 343
PB  - Wiley
CY  - Hoboken
ER  - 
TY  - JOUR
A1  - Tracy, Rosemarie
A1  - Heide, Judith
A1  - Wahl, Michael
A1  - Triarchi-Herrmann, Vassilia
A1  - Grimm, Angela
A1  - Wotschack, Christiane
A1  - Kulik, Sylvia
A1  - Frank, Ulrike
A1  - Klassert, Annegret
A1  - Gagarina, Natalʹja Vladimirovna
A1  - Kauschke, Christina
A1  - Eicher, Iris
A1  - Tsakmaki, Barbara
A1  - Akkaya, Zeynep
A1  - Castillo, Esmeralda
A1  - Groba, Agnes
A1  - Höhle, Barbara
A1  - Miertsch, Barbara
A1  - Hubert, Anja
A1  - Sauerland, Uli
A1  - Schröder, Caroline
A1  - Stadie, Nicole
A1  - Wittler, Marion
A1  - Berendes, Karin
A1  - Gottal, Stephanie
A1  - Grabherr, Britta
A1  - Zaps, Jennifer
A1  - Ptok, Martin
A1  - Hanne, Sandra
A1  - Sekerina, Irina A.
A1  - Vasishth, Shravan
A1  - Burchert, Frank
A1  - De Bleser, Ria
A1  - Kleissendorf, Barbara
A1  - Jaecks, Petra
A1  - Stenneken, Prisca
A1  - Fischer, Ivette
A1  - Moedebeck, Petra
ED  - Heide, Judith
ED  - Hanne, Sandra
ED  - Brandt-Kobele, Oda-Christina
ED  - Fritzsche, Tom
T1  - Spektrum Patholinguistik = Schwerpunktthema: Ein Kopf - Zwei Sprachen : Mehrsprachigkeit in Forschung und Therapie
N2  - "Spektrum Patholinguistik" (Band 2) ist der Tagungsband zum 2. Herbsttreffen Patholinguistik, das der Verband für Patholinguistik (vpl) e.V. am 22.11.2008 an der Universität Potsdam veranstaltet hat. Zum Schwerpunktthema "Ein Kopf - Zwei Sprachen: Mehrsprachigkeit in Forschung und Therapie" sind die drei Hauptvorträge und vier Abstracts von Posterpräsentationen veröffentlicht. Desweiteren enthält der Tagungsband freie Beiträge, u.a. zu Satzverarbeitung und Agrammatismus, Lesestrategien und LRS, Prosodie-Entwicklung, kindlichen Aphasien, Dysphagie-Therapie sowie zu kognitiven Defiziten bei älteren Menschen.
N2  - "Spektrum Patholinguistik" (vol. 2) contains the proceedings of the "2nd Herbsttreffen Patholinguistik" which was held at the University of Potsdam on November 22, 2008. Many talks and posters focused on bilingual language processing in normal and impaired speakers. The other contributions covered various topics, e.g. sentence processing and agrammatism, reading strategies and developmental dyslexia, language development, childhood aphasia, dysphagia therapy and cognitive skills in aging.
T3  - Spektrum Patholinguistik - 2 
KW  - Patholinguistik
KW  - Bilingualismus
KW  - Mehrsprachigkeit
KW  - Sprachtherapie
KW  - Sprachförderung
KW  - patholinguistics
KW  - bilingualism
KW  - speech and language therapy
Y1  - 2009
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-30451
SN  - 978-3-940793-89-8
SN  - 1869-3822
SN  - 1866-9433
IS  - 2
PB  - Universitätsverlag Potsdam
CY  - Potsdam
ER  -