TY  - JOUR
A1  - Woodhouse, Jason Nicholas
A1  - Makower, A. Katharina
A1  - Yeung, Anna C. Y.
A1  - Ongley, Sarah E.
A1  - Micallef, Melinda L.
A1  - Moffitt, Michelle C.
A1  - Neilan, Brett A.
T1  - Advances in genomics, transcriptomics and proteomics of toxin-producing cyanobacteria
JF  - Environmental microbiology reports
N2  - A common misconception persists that the genomes of toxic and non-toxic cyanobacterial strains are largely conserved with the exception of the presence or absence of the genes responsible for toxin production. Implementation of -omics era technologies has challenged this paradigm, with comparative analyses providing increased insight into the differences between strains of the same species. The implementation of genomic, transcriptomic and proteomic approaches has revealed distinct profiles between toxin-producing and non-toxic strains. Further, metagenomics and metaproteomics highlight the genomic potential and functional state of toxic bloom events over time. In this review, we highlight how these technologies have shaped our understanding of the complex relationship between these molecules, their producers and the environment at large within which they persist.
Y1  - 2016
U6  - https://doi.org/10.1111/1758-2229.12366
SN  - 1758-2229
VL  - 8
SP  - 3
EP  - 13
PB  - Wiley-Blackwell
CY  - Hoboken
ER  - 
TY  - JOUR
A1  - Makower, A. Katharina
A1  - Schuurmans, J. Merijn
A1  - Groth, Detlef
A1  - Zilliges, Yvonne
A1  - Matthijs, Hans C. P.
A1  - Dittmann-Thünemann, Elke
T1  - Transcriptomics-Aided dissection of the intracellular and extracellular roles of microcystin in microcystis aeruginosa PCC 7806
JF  - Applied and environmental microbiology
N2  - Recent studies have provided evidence for both intracellular and extracellular roles of the potent hepatotoxin microcystin (MC) in the bloom-forming cyanobacterium Microcystis. Here, we surveyed transcriptomes of the wild-type strain M. aeruginosa PCC 7806 and the microcystin-deficient Delta mcyB mutant under low light conditions with and without the addition of external MC of the LR variant (MC-LR). Transcriptomic data acquired by microarray and quantitative PCR revealed substantial differences in the relative expression of genes of the central intermediary metabolism, photosynthesis, and energy metabolism. In particular, the data provide evidence for a lower photosystem I (PSI)-to-photosystem II (PSII) ratio and a more pronounced carbon limitation in the microcystin-deficient mutant. Interestingly, only 6% of the transcriptional differences could be complemented by external microcystin-LR addition. This MC signaling effect was seen exclusively for genes of the secondary metabolism category. The orphan polyketide synthase gene cluster IPF38-51 was specifically downregulated in response to external MC-LR under low light. Our data suggest a hierarchical and light-dependent cross talk of secondary metabolites and support both an intracellular and an extracellular role of MC in Microcystis.
Y1  - 2015
U6  - https://doi.org/10.1128/AEM.02601-14
SN  - 0099-2240
SN  - 1098-5336
VL  - 81
IS  - 2
SP  - 544
EP  - 554
PB  - American Society for Microbiology
CY  - Washington
ER  -