TY - JOUR A1 - Spengler, D. A1 - Obata, M. A1 - Hirajima, T. A1 - Ottolini, L. A1 - Ohfuji, H. A1 - Tamura, A. A1 - Arai, S. T1 - Exsolution of garnet and clinopyroxene from High-Al Pyroxenes in Xugou Peridotite, Eastern China JF - Journal of petrology N2 - Serpentinized massif peridotite in the Xugou, Su-Lu ultrahigh-pressure (UHP) metamorphic belt, eastern China, preserves texturally old (porphyroclastic) ortho- and clinopyroxene with up to two generations of lamellae of garnet, clinopyroxene and Mg-chromite. Their crystallographic orientation with respect to the host pyroxene is consistent with an origin by solid-state exsolution. Comparison of integrated mineral chemistry with simplified and natural chemical datasets suggests that both aluminous precursor pyroxenes were in equilibrium at a minimum pressure of similar to 4 GPa and within a temperature range of about 1300-1500 degrees C. Steep isopleths of Ca in orthopyroxene imply that exsolution occurred during cooling. Al diffusion modelling suggests growth of widely spaced lamellae in orthopyroxene down to about 900 degrees C. Integrated Al contents between wide lamellae record a minimum of 4 GPa pressure during cooling. Compositionally uniform exsolved minerals were formed at 4 center dot 3 +/- 0 center dot 3 GPa and 730 +/- 30 degrees C and reflect a cratonic geotherm with about 33 mW m(-2) surface heat flow. The peridotite matrix mineral assemblage of olivine + orthopyroxene +/- garnet +/- Mg-chromite +/- clinopyroxene +/- phlogopite records strain-induced recrystallization that partially to completely replaced precursor porphyroclasts. The recrystallized minerals lack lamellar exsolution. Recrystallized orthopyroxene, with Al2O3 at 0 center dot 13 wt %, indicates conditions of 5 center dot 5 +/- 0 center dot 3 GPa and 760 +/- 30 degrees C, which are higher-grade metamorphic conditions than those preserved in the chemically equilibrated exsolution microstructures. Both estimates overlap with the range reported for the Early Mesozoic UHP metamorphism in the region (4 center dot 0-6 center dot 7 GPa and 760-970 degrees C). Major element melt models applied to previously published Xugou peridotite data suggest high degrees of melt extraction (30-35 %) in the garnet peridotite stability field (3-4 center dot 5 GPa) until garnet and clinopyroxene exhaustion. Coincidence in pressure and in the order of temperature of equilibration of precursor pyroxenes and peridotite melting implies that peridotite formation occurred at similar to 135 km depth in the subcontinental lithospheric mantle (SCLM) beneath the Archaean North China Craton. Subsequent refertilization, mineral exsolution and chemical re-equilibration during long-term cooling in the SCLM occurred prior to deformation and incorporation of the mantle fragment into the continental crust during UHP metamorphism at a minimum depth of 170 km. Because the Xugou precursor pyroxenes and peridotite formed at depths greater than the regional SCLM (c. 90 km), we infer that the orogenic peridotite massif formed part of the former hanging wall of the Archaean SCLM, which delaminated after the Late Mesozoic. KW - Archaean SCLM KW - Grt-Pyx exsolution KW - orogenic peridotite KW - UHP metamorphism Y1 - 2012 U6 - https://doi.org/10.1093/petrology/egs023 SN - 0022-3530 VL - 53 IS - 7 SP - 1477 EP - 1504 PB - Oxford Univ. Press CY - Oxford ER - TY - JOUR A1 - Gechev, Tsanko S. A1 - Benina, Maria A1 - Obata, Toshihiro A1 - Tohge, Takayuki A1 - Neerakkal, Sujeeth A1 - Minkov, Ivan A1 - Hille, Jacques A1 - Temanni, Mohamed-Ramzi A1 - Marriott, Andrew S. A1 - Bergström, Ed A1 - Thomas-Oates, Jane A1 - Antonio, Carla A1 - Müller-Röber, Bernd A1 - Schippers, Jos H. M. A1 - Fernie, Alisdair R. A1 - Toneva, Valentina T1 - Molecular mechanisms of desiccation tolerance in the resurrection glacial relic Haberlea rhodopensis JF - Cellular and molecular life sciences N2 - Haberlea rhodopensis is a resurrection plant with remarkable tolerance to desiccation. Haberlea exposed to drought stress, desiccation, and subsequent rehydration showed no signs of damage or severe oxidative stress compared to untreated control plants. Transcriptome analysis by next-generation sequencing revealed a drought-induced reprogramming, which redirected resources from growth towards cell protection. Repression of photosynthetic and growth-related genes during water deficiency was concomitant with induction of transcription factors (members of the NAC, NF-YA, MADS box, HSF, GRAS, and WRKY families) presumably acting as master switches of the genetic reprogramming, as well as with an upregulation of genes related to sugar metabolism, signaling, and genes encoding early light-inducible (ELIP), late embryogenesis abundant (LEA), and heat shock (HSP) proteins. At the same time, genes encoding other LEA, HSP, and stress protective proteins were constitutively expressed at high levels even in unstressed controls. Genes normally involved in tolerance to salinity, chilling, and pathogens were also highly induced, suggesting a possible cross-tolerance against a number of abiotic and biotic stress factors. A notable percentage of the genes highly regulated in dehydration and subsequent rehydration were novel, with no sequence homology to genes from other plant genomes. Additionally, an extensive antioxidant gene network was identified with several gene families possessing a greater number of antioxidant genes than most other species with sequenced genomes. Two of the transcripts most abundant during all conditions encoded catalases and five more catalases were induced in water-deficient samples. Using the pharmacological inhibitor 3-aminotriazole (AT) to compromise catalase activity resulted in increased sensitivity to desiccation. Metabolome analysis by GC or LC-MS revealed accumulation of sucrose, verbascose, spermidine, and gamma-aminobutyric acid during drought, as well as particular secondary metabolites accumulating during rehydration. This observation, together with the complex antioxidant system and the constitutive expression of stress protective genes suggests that both constitutive and inducible mechanisms contribute to the extreme desiccation tolerance of H. rhodopensis. KW - Antioxidant genes KW - Catalase KW - Desiccation tolerance KW - Drought stress KW - Metabolome analysis KW - Resurrection plants Y1 - 2013 U6 - https://doi.org/10.1007/s00018-012-1155-6 SN - 1420-682X VL - 70 IS - 4 SP - 689 EP - 709 PB - Springer CY - Basel ER - TY - JOUR A1 - Schmidt, Romy A1 - Mieulet, Delphine A1 - Hubberten, Hans-Michael A1 - Obata, Toshihiro A1 - Höfgen, Rainer A1 - Fernie, Alisdair R. A1 - Fisahn, Joachim A1 - Segundo, Blanca San A1 - Guiderdoni, Emmanuel A1 - Schippers, Jos H. M. A1 - Müller-Röber, Bernd T1 - Salt-responsive ERF1 regulates reactive oxygen species-dependent signaling during the initial response to salt stress in rice JF - The plant cell N2 - Early detection of salt stress is vital for plant survival and growth. Still, the molecular processes controlling early salt stress perception and signaling are not fully understood. Here, we identified SALT-RESPONSIVE ERF1 (SERF1), a rice (Oryza sativa) transcription factor (TF) gene that shows a root-specific induction upon salt and hydrogen peroxide (H2O2) treatment. Loss of SERF1 impairs the salt-inducible expression of genes encoding members of a mitogen-activated protein kinase (MAPK) cascade and salt tolerance-mediating TFs. Furthermore, we show that SERF1-dependent genes are H2O2 responsive and demonstrate that SERF1 binds to the promoters of MAPK KINASE KINASE6 (MAP3K6), MAPK5, DEHYDRATION-RESPONSIVE ELEMENT BINDING2A (DREB2A), and ZINC FINGER PROTEIN179 (ZFP179) in vitro and in vivo. SERF1 also directly induces its own gene expression. In addition, SERF1 is a phosphorylation target of MAPK5, resulting in enhanced transcriptional activity of SERF1 toward its direct target genes. In agreement, plants deficient for SERF1 are more sensitive to salt stress compared with the wild type, while constitutive overexpression of SERF1 improves salinity tolerance. We propose that SERF1 amplifies the reactive oxygen species-activated MAPK cascade signal during the initial phase of salt stress and translates the salt-induced signal into an appropriate expressional response resulting in salt tolerance. Y1 - 2013 U6 - https://doi.org/10.1105/tpc.113.113068 SN - 1040-4651 VL - 25 IS - 6 SP - 2115 EP - 2131 PB - American Society of Plant Physiologists CY - Rockville ER - TY - JOUR A1 - Schmidt, Romy A1 - Schippers, Jos H. M. A1 - Mieulet, Delphine A1 - Obata, Toshihiro A1 - Fernie, Alisdair R. A1 - Guiderdoni, Emmanuel A1 - Müller-Röber, Bernd T1 - Multipass, a rice R2R3-type MYB transcription factor, regulates adaptive growth by integrating multiple hormonal pathways JF - The plant journal N2 - Growth regulation is an important aspect of plant adaptation during environmental perturbations. Here, the role of MULTIPASS (OsMPS), an R2R3-type MYB transcription factor of rice, was explored. OsMPS is induced by salt stress and expressed in vegetative and reproductive tissues. Over-expression of OsMPS reduces growth under non-stress conditions, while knockdown plants display increased biomass. OsMPS expression is induced by abscisic acid and cytokinin, but is repressed by auxin, gibberellin and brassinolide. Growth retardation caused by OsMPS over-expression is partially restored by auxin application. Expression profiling revealed that OsMPS negatively regulates the expression of EXPANSIN (EXP) and cell-wall biosynthesis as well as phytohormone signaling genes. Furthermore, the expression of OsMPS-dependent genes is regulated by auxin, cytokinin and abscisic acid. Moreover, we show that OsMPS is a direct upstream regulator of OsEXPA4, OsEXPA8, OsEXPB2, OsEXPB3, OsEXPB6 and the endoglucanase genes OsGLU5 and OsGLU14. The multiple responses of OsMPS and its target genes to various hormones suggest an integrative function of OsMPS in the cross-talk between phytohormones and the environment to regulate adaptive growth. KW - development KW - expansin KW - transcription KW - Oryza sativa KW - hormone KW - abiotic stress Y1 - 2013 U6 - https://doi.org/10.1111/tpj.12286 SN - 0960-7412 SN - 1365-313X VL - 76 IS - 2 SP - 258 EP - 273 PB - Wiley-Blackwell CY - Hoboken ER -