TY - GEN A1 - Lang, Judith A1 - Bohn, Patrick A1 - Bhat, Hilal A1 - Jastrow, Holger A1 - Walkenfort, Bernd A1 - Cansiz, Feyza A1 - Fink, Julian A1 - Bauer, Michael A1 - Schumacher, Fabian A1 - Kleuser, Burkhard A1 - Lang, Karl S. T1 - Acid ceramidase of macrophages traps herpes simplex virus in multivesicular bodies and protects from severe disease T2 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe N2 - Macrophages have important protective functions during infection with herpes simplex virus type 1 (HSV-1). However, molecular mechanisms that restrict viral propagation and protect from severe disease are unclear. Here we show that macrophages take up HSV-1 via endocytosis and transport the virions into multivesicular bodies (MVBs). In MVBs, acid ceramidase (aCDase) converts ceramide into sphingosine and increases the formation of sphingosine-rich intraluminal vesicles (ILVs). Once HSV-1 particles reach MVBs, sphingosine-rich ILVs bind to HSV-1 particles, which restricts fusion with the limiting endosomal membrane and prevents cellular infection. Lack of aCDase in macrophage cultures or in Asah1(-/-) mice results in replication of HSV-1 and Asah1(-/-) mice die soon after systemic or intravaginal inoculation. The treatment of macrophages with sphingosine enhancing compounds blocks HSV-1 propagation, suggesting a therapeutic potential of this pathway. In conclusion, aCDase loads ILVs with sphingosine, which prevents HSV-1 capsids from penetrating into the cytosol. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1400 KW - immunology KW - infection KW - membrane fusion KW - phagocytosis KW - sphingolipids Y1 - 2020 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-515661 SN - 1866-8372 IS - 1 ER - TY - JOUR A1 - Lang, Judith A1 - Bohn, Patrick A1 - Bhat, Hilal A1 - Jastrow, Holger A1 - Walkenfort, Bernd A1 - Cansiz, Feyza A1 - Fink, Julian A1 - Bauer, Michael A1 - Schumacher, Fabian A1 - Kleuser, Burkhard A1 - Lang, Karl S. T1 - Acid ceramidase of macrophages traps herpes simplex virus in multivesicular bodies and protects from severe disease JF - Nature Communications N2 - Macrophages have important protective functions during infection with herpes simplex virus type 1 (HSV-1). However, molecular mechanisms that restrict viral propagation and protect from severe disease are unclear. Here we show that macrophages take up HSV-1 via endocytosis and transport the virions into multivesicular bodies (MVBs). In MVBs, acid ceramidase (aCDase) converts ceramide into sphingosine and increases the formation of sphingosine-rich intraluminal vesicles (ILVs). Once HSV-1 particles reach MVBs, sphingosine-rich ILVs bind to HSV-1 particles, which restricts fusion with the limiting endosomal membrane and prevents cellular infection. Lack of aCDase in macrophage cultures or in Asah1(-/-) mice results in replication of HSV-1 and Asah1(-/-) mice die soon after systemic or intravaginal inoculation. The treatment of macrophages with sphingosine enhancing compounds blocks HSV-1 propagation, suggesting a therapeutic potential of this pathway. In conclusion, aCDase loads ILVs with sphingosine, which prevents HSV-1 capsids from penetrating into the cytosol. KW - immunology KW - infection KW - membrane fusion KW - phagocytosis KW - sphingolipids Y1 - 2020 U6 - https://doi.org/10.1038/s41467-020-15072-8 SN - 2041-1723 VL - 11 IS - 1 SP - 1 EP - 15 PB - Nature Publishing Group UK CY - London ER - TY - JOUR A1 - Clahsen, Harald A1 - Siegmüller, Julia A1 - Penke, Martina A1 - Schröder, Astrid A1 - Hofmann, Janine A1 - Holzgrefe-Lang, Julia A1 - Skerra, Antje A1 - Adani, Flavia A1 - Gagarina, Natalʹja Vladimirovna A1 - Schröter, Carolin A1 - Frieg, Hendrike A1 - Belke, Eva A1 - Schwab, Susanne A1 - Seifert, Susanne A1 - Watko, Petra A1 - Obendrauf, Tanja A1 - Trauntschnig, Mike A1 - Gasteiger-Klicpera, Barbara A1 - Adelt, Anne A1 - Hanne, Sandra A1 - Burchert, Frank A1 - Swietza, Romy A1 - Doppelbauer, Lea A1 - Dralle, Jenny A1 - Purat, Patricia A1 - Webersinke, Dorothea A1 - Schwytay, Jeannine A1 - Stadie, Nicole A1 - Hoppe, Carina A1 - Heide, Judith A1 - Marusch, Tina A1 - von der Malsburg, Titus Raban A1 - Bastiaanse, Roelien A1 - Schultheiss, Corinna A1 - Nahrstaedt, Holger A1 - Schauer, Thomas A1 - Seidl, Rainer Ottis A1 - Rath, Elisa ED - Fritzsche, Tom ED - Meyer, Corinna B. ED - Adelt, Anne ED - Roß, Jennifer T1 - Spektrum Patholinguistik = Schwerpunktthema: Labyrinth Grammatik: Therapie von syntaktischen Störungen bei Kindern und Erwachsenen N2 - Das Herbsttreffen Patholinguistik wird seit 2007 jährlich vom Verband für Patholinguistik e.V. (vpl) durchgeführt. Das 6. Herbsttreffen mit dem Schwerpunktthema "Labyrinth Grammatik: Therapie von syntaktischen Störungen bei Kindern und Erwachsenen" fand am 17.11.2012 in Potsdam statt. Im vorliegenden Tagungsband finden sich alle Beiträge der Veranstaltung: die vier Hauptvorträge zum Schwerpunkthema, die Vorträge aus Praxis und Forschung von vier Patholinguistinnen in der Reihe Spektrum Patholinguistik sowie die Abstracts der Posterpräsentation. N2 - The 'Herbsttreffen Patholinguistik' is an annual conference organized by the Association for Patholinguistics (Verband für Patholinguistik e.V./vpl) since 2007. The 6th Herbsttreffen with its theme "Labyrinth Grammar: Therapy of Syntactic Disorders in Children and Adults" took place on November 17th, 2012 in Potsdam. These proceedings contain all contributions of the meeting: the four keynote talks, the talks on speech/language therapy and research in the section "Spektrum Patholinguistik" by four patholinguists as well as the abstracts of the poster session. T3 - Spektrum Patholinguistik - 6 KW - Patholinguistik KW - syntaktische Störungen KW - Aphasie KW - Agrammatismus KW - Dysgrammatismus KW - patholinguistics KW - speech/language therapy KW - syntactic disorders KW - aphasia KW - agrammatism Y1 - 2013 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-67659 SN - 978-3-86956-270-4 SN - 1869-3822 SN - 1866-9433 IS - 6 PB - Universitätsverlag Potsdam CY - Potsdam ER - TY - JOUR A1 - Stadie, Nicole A1 - Ulrich, Tanja A1 - Glück, Christian W. A1 - Richter, Kerstin A1 - Klassert, Annegret A1 - Krause, Carina Denise A1 - Wagner, Susanne A1 - Holzgrefe-Lang, Julia A1 - Lorenz, Elisa A1 - Oelze, Vera A1 - Schütz, Vivien A1 - Peinhardt, Ulrich A1 - Laßmann, Inga A1 - Hanne, Sandra A1 - Wollenberg, Maxi A1 - Düring, Sarah A1 - Laubscheer, Ann-Katrin A1 - Heide, Judith A1 - Gruhn, Sophie A1 - Segers, Eliane A1 - Keuning, Jos A1 - Verhoeven, Ludo A1 - Obry, Svenja A1 - Bohn, Bianca A1 - Neise, Romy A1 - Pregla, Dorothea A1 - Wiehe, Lea A1 - Weiland, Katharina A1 - Wahl, Michael ED - Fritzsche, Tom ED - Breitenstein, Sarah ED - Wunderlich, Hanna ED - Ferchland, Lisa ED - Krug, Ragna T1 - Spektrum Patholinguistik Band 13. Schwerpunktthema: Nur ein Wort? Diagnostik und Therapie von Wortabrufstörungen bei Kindern und Erwachsenen T2 - Spektrum Patholinguistik N2 - Das 13. Herbsttreffen Patholinguistik mit dem Schwerpunktthema »Nur ein Wort? Diagnostik und Therapie von Wortabrufstörungen bei Kindern und Erwachsenen« fand am 16.11.2019 in Potsdam statt. Das Herbsttreffen wird seit 2007 jährlich vom Verband für Patholinguistik e.V. (vpl) in Kooperation mit dem Deutschen Bundesverband für akademische Sprachtherapie und Logopädie (dbs) und der Universität Potsdam durchgeführt. Der vorliegende Tagungsband beinhaltet die Hauptvorträge zum Schwerpunktthema sowie die Beiträge der Kurzvorträge im »Spektrum Patholinguistik« und der Posterpräsentationen zu weiteren Themen aus der sprachtherapeutischen Forschung und Praxis. N2 - The Thirteenth Autumn Meeting Patholinguistics with its main topic »Just a word? Diagnostics and therapy of word-finding difficulties in children and adults« took place in Potsdam on the 16th of November 2019. This annual meeting has been organised since 2007 by the Association for Patholinguistics (vpl) in cooperation with the German Federal Association for Academic Speech/Language Therapy and Logopaedics (dbs) and the University of Potsdam. The present proceedings feature the keynote presentations on the main topic as well as the short presentations from the section »Spectrum Patholinguistics« and from the poster session covering a broad range of areas in research and practice of speech/language therapy. T3 - Spektrum Patholinguistik - 13 KW - Patholinguistik KW - Sprachtherapie KW - Wortfindungsstörungen KW - Wortabruf KW - lexikalisch-semantische Verarbeitung KW - patholinguistics KW - speech/language therapy KW - word-finding difficulties KW - word retrieval KW - lexical-semantic processing Y1 - 2020 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-460777 SN - 978-3-86956-488-3 SN - 1866-9433 SN - 1869-3822 IS - 13 PB - Universitätsverlag Potsdam CY - Potsdam ER -