TY - JOUR A1 - Gutbier, Birgitt A1 - Schönrock, Stefanie M. A1 - Ehrler, Carolin A1 - Haberberger, Rainer A1 - Dietert, Kristina A1 - Gruber, Achim D. A1 - Kummer, Wolfgang A1 - Michalick, Laura A1 - Kuebler, Wolfgang M. A1 - Hocke, Andreas C. A1 - Szymanski, Kolja A1 - Letsiou, Eleftheria A1 - Lüth, Anja A1 - Schumacher, Fabian A1 - Kleuser, Burkhard A1 - Mitchell, Timothy J. A1 - Bertrams, Wilhelm A1 - Schmeck, Bernd A1 - Treue, Denise A1 - Klauschen, Frederick A1 - Bauer, Torsten T. A1 - Tönnies, Mario A1 - Weissmann, Norbert A1 - Hippenstiel, Stefan A1 - Suttorp, Norbert A1 - Witzenrath, Martin T1 - Sphingosine Kinase 1 Regulates Inflammation and Contributes to Acute Lung Injury in Pneumococcal Pneumonia via the Sphingosine-1-Phosphate Receptor 2 JF - Critical care medicine N2 - Objectives: Severe pneumonia may evoke acute lung injury, and sphingosine-1-phosphate is involved in the regulation of vascular permeability and immune responses. However, the role of sphingosine-1-phosphate and the sphingosine-1-phosphate producing sphingosine kinase 1 in pneumonia remains elusive. We examined the role of the sphingosine-1-phosphate system in regulating pulmonary vascular barrier function in bacterial pneumonia. Design: Controlled, in vitro, ex vivo, and in vivo laboratory study. Subjects: Female wild-type and SphK1-deficient mice, 8-10 weeks old. Human postmortem lung tissue, human blood-derived macrophages, and pulmonary microvascular endothelial cells. Interventions: Wild-type and SphK1-deficient mice were infected with Streptococcus pneumoniae. Pulmonary sphingosine-1-phosphate levels, messenger RNA expression, and permeability as well as lung morphology were analyzed. Human blood-derived macrophages and human pulmonary microvascular endothelial cells were infected with S. pneumoniae. Transcellular electrical resistance of human pulmonary microvascular endothelial cell monolayers was examined. Further, permeability of murine isolated perfused lungs was determined following exposition to sphingosine-1-phosphate and pneumolysin. Measurements and Main Results: Following S. pneumoniae infection, murine pulmonary sphingosine-1-phosphate levels and sphingosine kinase 1 and sphingosine-1-phosphate receptor 2 expression were increased. Pneumonia-induced lung hyperpermeability was reduced in SphK1(-/-) mice compared with wild-type mice. Expression of sphingosine kinase 1 in macrophages recruited to inflamed lung areas in pneumonia was observed in murine and human lungs. S. pneumoniae induced the sphingosine kinase 1/sphingosine-1-phosphate system in blood-derived macrophages and enhanced sphingosine-1-phosphate receptor 2 expression in human pulmonary microvascular endothelial cell in vitro. In isolated mouse lungs, pneumolysin-induced hyperpermeability was dose dependently and synergistically increased by sphingosine-1-phosphate. This sphingosine-1-phosphate-induced increase was reduced by inhibition of sphingosine-1-phosphate receptor 2 or its downstream effector Rho-kinase. Conclusions: Our data suggest that targeting the sphingosine kinase 1-/sphingosine-1-phosphate-/sphingosine-1-phosphate receptor 2-signaling pathway in the lung may provide a novel therapeutic perspective in pneumococcal pneumonia for prevention of acute lung injury. KW - acute lung injury KW - pneumococcal pneumonia KW - sphingosine kinase 1 KW - sphingosine-1-phosphate KW - sphingosine-1-phosphate receptor 2 Y1 - 2018 U6 - https://doi.org/10.1097/CCM.0000000000002916 SN - 0090-3493 SN - 1530-0293 VL - 46 IS - 3 SP - e258 EP - e267 PB - Lippincott Williams & Wilkins CY - Philadelphia ER - TY - JOUR A1 - Jage, Horst A1 - Kummer, Volker A1 - Illig, Hubert A1 - Petrick, Wolfgang T1 - Beitrag zur Kenntnis phytoparasitischer Kleinpilze in der Niederlausitz (Land Brandenburg) : Teil 2 Y1 - 2006 ER - TY - JOUR A1 - Fischer, Wolfgang A1 - Kummer, Volker A1 - Pötsch, Joachim T1 - Zur Vegetation des Feuchtgebietes Internationaler Bedeutung (FIB) Untere Havel Y1 - 1994 ER - TY - JOUR A1 - Fischer, Wolfgang A1 - Kummer, Volker T1 - Zur Verbreitung und Soziologie von S colochloa festucacea L : in Nordostdeutschland und seine Unterscheidung von ähnlichen Gräsern nach vegetativen Merkmalen Y1 - 1994 ER - TY - JOUR A1 - Fischer, Wolfgang A1 - Kummer, Volker T1 - Ein Beitrag zur Pilzflora der Höllenberge bei Langengrassau Y1 - 1995 ER - TY - JOUR A1 - Baral, Hans Otto A1 - Rönsch, Peter A1 - Richter, Udo A1 - Urban, Alexander A1 - Kruse, Julia A1 - Bemmann, Martin A1 - Kummer, Volker A1 - Javier Valencia, Francisco A1 - Huth, Wolfgang T1 - Schroeteria decaisneana, S. poeltii, and Ciboria ploettneriana (Sclerotiniaceae, Helotiales, Ascomycota), three parasites on Veronica seeds BT - first report of teleomorphs in Schroeteria JF - Mycological progress : international journal of the German Mycological Society N2 - Ciboria ploettneriana, Schroeteria decaisneana, and S. poeltii produce morphologically very similar apothecia emerging from fallen stromatized seeds of Veronica spp., the former two on V. hederifolia agg. in temperate central Europe and S. poeltii on V. cymbalaria in mediterranean southern Europe. They are described and illustrated in detail based on fresh collections or moist chamber cultures of infected seeds. A key is provided to differentiate the three species from their teleomorphs. For the first time, connections between two teleomorphs and two Schroeteria anamorphs are reported. Members of the anamorph-typified genus Schroeteria are known as host-specific plant parasites that infect seeds of different Veronica spp. In earlier times, they were classified in the Ustilaginales (Basidiomycota), but since more than 30 years, they are referred to as false smut fungi producing smut-like chlamydospores, based on light microscopic and ultrastructural studies which referred them to the Sclerotiniaceae (Helotiales). During the present study, rDNA sequences were obtained for the first time from chlamydospores of Schroeteria bornmuelleri (on V. rubrifolia), S. decaisneana (on V. hederifolia), S. delastrina (generic type, on V. arvensis), and S. poeltii (on V. cymbalaria) and from apothecia of C. ploettneriana, S. decaisneana, and S. poeltii. As a result, the anamorph-teleomorph connection could be established for S. decaisneana and S. poeltii by a 100% ITS similarity, whereas C. ploettneriana could not be connected to a smut-like anamorph. Ciboria ploettneriana in the here-redefined sense clustered in our combined phylogenetic analyses of ITS and LSU in relationship of Sclerotinia s.l., Botrytis, and Myriosclerotinia rather than Ciboria, but its placement was not supported. Its affiliation in Ciboria was retained until a better solution is found. Also Schroeteria poeltii clustered unresolved in this relationship but with a much higher molecular distance. The remaining three Schroeteria spp. formed a strongly supported monophyletic group, here referred to as "Schroeteria core clade", which clustered with medium to high support as a sister clade of Monilinia jezoensis, a member of the Monilinia alpina group of section Disjunctoriae. We observed ITS distances of 5-6.3% among members of the Schroeteria core clade, but 13.8-14.7% between this clade and S. poeltii, which appears to be correlated with the deviating chlamydospore morphology of S. poeltii. Despite its apparent paraphyly, Schroeteria is accepted here in a wide sense as a genus distinct from Monilinia, particularly because of its very special anamorphs. A comparable heterogeneity in rDNA analyses was observed in Monilinia and other genera of Sclerotiniaceae. Such apparent heterogeneity should be met with skepticism, however, because the inclusion of protein-coding genes in phylogenetic analyses resulted in a monophyletic genus Monilinia. More sclerotiniaceous taxa should be analysed for protein-coding genes in the future, including Schroeteria. Four syntype specimens of Ciboria ploettneriana in B were reexamined in the present study, revealing a mixture of the two species growing on V. hederifolia agg. Based on its larger ascospores in comparison with S. decaisneana, a lectotype is proposed for C. ploettneriana. KW - anamorph-teleomorph connection KW - plant parasite KW - false smut fungi KW - seed KW - infection KW - Veronica hederifolia KW - Veronica cymbalaria Y1 - 2022 U6 - https://doi.org/10.1007/s11557-021-01742-4 SN - 1617-416X SN - 1861-8952 VL - 21 IS - 1 SP - 359 EP - 407 PB - Springer CY - Berlin ; Heidelberg ER - TY - JOUR A1 - Burkart, Michael A1 - Kummer, Volker A1 - Fischer, Wolfgang T1 - Floristische Neu- und Wiederfunde im Gebiet der Unteren Havel Y1 - 1995 ER - TY - JOUR A1 - Scherber, Christoph A1 - Eisenhauer, Nico A1 - Weisser, Wolfgang W. A1 - Schmid, Bernhard A1 - Voigt, Winfried A1 - Fischer, Markus A1 - Schukze, Ernst-Detlef A1 - Roscher, Christiane A1 - Weigelt, Alexandra A1 - Allan, Eric A1 - Beßler, Holger A1 - Bonkowski, Michael A1 - Buchmann, Nina A1 - Buscot, François A1 - Clement, Lars W. A1 - Ebeling, Anne A1 - Engels, Christof A1 - Halle, Stefan A1 - Kertscher, Ilona A1 - Klein, Alexandra-Maria A1 - Koller, Robert A1 - König, Stephan A1 - Kowalski, Esther A1 - Kummer, Volker A1 - Kuu, Annely A1 - Lange, Markus A1 - Lauterbach, Dirk T1 - Bottom-up effects of plant diversity on multitrophic interactions in a biodiversity experiment Y1 - 2010 UR - http://www.nature.com/nature/journal/v468/n7323/full/nature09492.html SN - 0028-0836 ER -