TY  - JOUR
A1  - Koschorreck, Matthias
A1  - Frömmichen, René
A1  - Herzsprung, Peter
A1  - Tittel, Jörg
A1  - Wendt-Potthoff, Katrin
T1  - Function of straw for in situ remediation of acidic mining lakes
Y1  - 2002
SN  - 0049-6979
ER  - 
TY  - JOUR
A1  - Klapper, H.
A1  - Boehrer, Bertram
A1  - Packroff, G.
A1  - Schultze, M.
A1  - Tittel, Jörg
A1  - Wendt-Potthoff, Katrin
T1  - Bergbaufolgegewässer
Y1  - 2001
ER  - 
TY  - JOUR
A1  - Dunker, Susanne
A1  - Boyd, Matthew
A1  - Durka, Walter
A1  - Erler, Silvio
A1  - Harpole, W. Stanley
A1  - Henning, Silvia
A1  - Herzschuh, Ulrike
A1  - Hornick, Thomas
A1  - Knight, Tiffany
A1  - Lips, Stefan
A1  - Mäder, Patrick
A1  - Švara, Elena Motivans
A1  - Mozarowski, Steven
A1  - Rakosy, Demetra
A1  - Römermann, Christine
A1  - Schmitt-Jansen, Mechthild
A1  - Stoof-Leichsenring, Kathleen
A1  - Stratmann, Frank
A1  - Treudler, Regina
A1  - Virtanen, Risto
A1  - Wendt-Potthoff, Katrin
A1  - Wilhelm, Christian
T1  - The potential of multispectral imaging flow cytometry for environmental monitoring
JF  - Cytometry part A
N2  - Environmental monitoring involves the quantification of microscopic cells and particles such as algae, plant cells, pollen, or fungal spores. Traditional methods using conventional microscopy require expert knowledge, are time-intensive and not well-suited for automated high throughput. Multispectral imaging flow cytometry (MIFC) allows measurement of up to 5000 particles per second from a fluid suspension and can simultaneously capture up to 12 images of every single particle for brightfield and different spectral ranges, with up to 60x magnification. The high throughput of MIFC has high potential for increasing the amount and accuracy of environmental monitoring, such as for plant-pollinator interactions, fossil samples, air, water or food quality that currently rely on manual microscopic methods. Automated recognition of particles and cells is also possible, when MIFC is combined with deep-learning computational techniques. Furthermore, various fluorescence dyes can be used to stain specific parts of the cell to highlight physiological and chemical features including: vitality of pollen or algae, allergen content of individual pollen, surface chemical composition (carbohydrate coating) of cells, DNA- or enzyme-activity staining. Here, we outline the great potential for MIFC in environmental research for a variety of research fields and focal organisms. In addition, we provide best practice recommendations.
KW  - environmental monitoring
KW  - imaging flow cytometry
KW  - plant traits
Y1  - 2022
U6  - https://doi.org/10.1002/cyto.a.24658
SN  - 1552-4922
SN  - 1552-4930
VL  - 101
IS  - 9
SP  - 782
EP  - 799
PB  - Wiley
CY  - Hoboken
ER  -