TY - JOUR A1 - Gossner, Martin M. A1 - Lewinsohn, Thomas M. A1 - Kahl, Tiemo A1 - Grassein, Fabrice A1 - Boch, Steffen A1 - Prati, Daniel A1 - Birkhofer, Klaus A1 - Renner, Swen C. A1 - Sikorski, Johannes A1 - Wubet, Tesfaye A1 - Arndt, Hartmut A1 - Baumgartner, Vanessa A1 - Blaser, Stefan A1 - Blüthgen, Nico A1 - Börschig, Carmen A1 - Buscot, Francois A1 - Diekötter, Tim A1 - Jorge, Leonardo Re A1 - Jung, Kirsten A1 - Keyel, Alexander C. A1 - Klein, Alexandra-Maria A1 - Klemmer, Sandra A1 - Krauss, Jochen A1 - Lange, Markus A1 - Müller, Jörg A1 - Overmann, Jörg A1 - Pasalic, Esther A1 - Penone, Caterina A1 - Perovic, David J. A1 - Purschke, Oliver A1 - Schall, Peter A1 - Socher, Stephanie A. A1 - Sonnemann, Ilja A1 - Tschapka, Marco A1 - Tscharntke, Teja A1 - Türke, Manfred A1 - Venter, Paul Christiaan A1 - Weiner, Christiane N. A1 - Werner, Michael A1 - Wolters, Volkmar A1 - Wurst, Susanne A1 - Westphal, Catrin A1 - Fischer, Markus A1 - Weisser, Wolfgang W. A1 - Allan, Eric T1 - Land-use intensification causes multitrophic homogenization of grassland communities JF - Nature : the international weekly journal of science N2 - Land-use intensification is a major driver of biodiversity loss(1,2). Alongside reductions in local species diversity, biotic homogenization at larger spatial scales is of great concern for conservation. Biotic homogenization means a decrease in beta-diversity (the compositional dissimilarity between sites). Most studies have investigated losses in local (alpha)-diversity(1,3) and neglected biodiversity loss at larger spatial scales. Studies addressing beta-diversity have focused on single or a few organism groups (for example, ref. 4), and it is thus unknown whether land-use intensification homogenizes communities at different trophic levels, above-and belowground. Here we show that even moderate increases in local land-use intensity (LUI) cause biotic homogenization across microbial, plant and animal groups, both above- and belowground, and that this is largely independent of changes in alpha-diversity. We analysed a unique grassland biodiversity dataset, with abundances of more than 4,000 species belonging to 12 trophic groups. LUI, and, in particular, high mowing intensity, had consistent effects on beta-diversity across groups, causing a homogenization of soil microbial, fungal pathogen, plant and arthropod communities. These effects were nonlinear and the strongest declines in beta-diversity occurred in the transition from extensively managed to intermediate intensity grassland. LUI tended to reduce local alpha-diversity in aboveground groups, whereas the alpha-diversity increased in belowground groups. Correlations between the alpha-diversity of different groups, particularly between plants and their consumers, became weaker at high LUI. This suggests a loss of specialist species and is further evidence for biotic homogenization. The consistently negative effects of LUI on landscape-scale biodiversity underscore the high value of extensively managed grasslands for conserving multitrophic biodiversity and ecosystem service provision. Indeed, biotic homogenization rather than local diversity loss could prove to be the most substantial consequence of land-use intensification. Y1 - 2016 U6 - https://doi.org/10.1038/nature20575 SN - 0028-0836 SN - 1476-4687 VL - 540 SP - 266 EP - + PB - Nature Publ. Group CY - London ER - TY - JOUR A1 - Kahl, Sandra A1 - Kappel, Christian A1 - Joshi, Jasmin Radha A1 - Lenhard, Michael T1 - Phylogeography of a widely distributed plant species reveals cryptic genetic lineages with parallel phenotypic responses to warming and drought conditions JF - Ecology and Evolution N2 - To predict how widely distributed species will perform under future climate change, it is crucial to understand and reveal their underlying phylogenetics. However, detailed information about plant adaptation and its genetic basis and history remains scarce and especially widely distributed species receive little attention despite their putatively high adaptability. To examine the adaptation potential of a widely distributed species, we sampled the model plant Silene vulgaris across Europe. In a greenhouse experiment, we exposed the offspring of these populations to a climate change scenario for central Europe and revealed the population structure through whole-genome sequencing. Plants were grown under two temperatures (18°C and 21°C) and three precipitation regimes (65, 75, and 90 mm) to measure their response in biomass and fecundity-related traits. To reveal the population genetic structure, ddRAD sequencing was employed for a whole-genome approach. We found three major genetic clusters in S. vulgaris from Europe: one cluster comprising Southern European populations, one cluster of Western European populations, and another cluster containing central European populations. Population genetic diversity decreased with increasing latitude, and a Mantel test revealed significant correlations between FST and geographic distances as well as between genetic and environmental distances. Our trait analysis showed that the genetic clusters significantly differed in biomass-related traits and in the days to flowering. However, half of the traits showed parallel response patterns to the experimental climate change scenario. Due to the differentiated but parallel response patterns, we assume that phenotypic plasticity plays an important role for the adaptation of the widely distributed species S. vulgaris and its intraspecific genetic lineages. KW - climate adaptation KW - ddRAD KW - Silene vulgaris Y1 - 2021 U6 - https://doi.org/10.1002/ece3.8103 SN - 2045-7758 VL - 11 IS - 20 SP - 13986 EP - 14002 PB - John Wiley & Sons, Inc. CY - Hoboken ER - TY - JOUR A1 - Zwickel, Theresa A1 - Kahl, Sandra A1 - Rychlik, Michael A1 - Müller, Marina E. H. T1 - Chemotaxonomy of Mycotoxigenic Small-Spored Alternaria Fungi BT - Do Multitoxin Mixtures Act as an Indicator for Species Differentiation? JF - Frontiers in microbiology N2 - Necrotrophic as well as saprophytic small-spored Altemaria (A.) species are annually responsible for major losses of agricultural products, such as cereal crops, associated with the contamination of food and feedstuff with potential health-endangering Altemaria toxins. Knowledge of the metabolic capabilities of different species-groups to form mycotoxins is of importance for a reliable risk assessment. 93 Altemaria strains belonging to the four species groups Alternaria tenuissima, A. arborescens, A. altemata, and A. infectoria were isolated from winter wheat kernels harvested from fields in Germany and Russia and incubated under equal conditions. Chemical analysis by means of an HPLC-MS/MS multi-Alternaria-toxin-method showed that 95% of all strains were able to form at least one of the targeted 17 non-host specific Altemaria toxins. Simultaneous production of up to 15 (modified) Altemaria toxins by members of the A. tenuissima, A. arborescens, A. altemata species-groups and up to seven toxins by A. infectoria strains was demonstrated. Overall tenuazonic acid was the most extensively formed mycotoxin followed by alternariol and alternariol mono methylether, whereas altertoxin I was the most frequently detected toxin. Sulfoconjugated modifications of alternariol, alternariol mono methylether, altenuisol and altenuene were frequently determined. Unknown perylene quinone derivatives were additionally detected. Strains of the species-group A. infectoria could be segregated from strains of the other three species-groups due to significantly lower toxin levels and the specific production of infectopyrone. Apart from infectopyrone, alterperylenol was also frequently produced by 95% of the A. infectoria strains. Neither by the concentration nor by the composition of the targeted Altemaria toxins a differentiation between the species-groups A. altemata, A. tenuissima and A. arborescens was possible. KW - small-spored Alternaria fungi KW - Alternaria species-groups KW - Alternaria mycotoxins KW - chemotaxonomy KW - secondary metabolite profiling KW - LC-MS/MS KW - wheat KW - perylene quinone derivatives Y1 - 2018 U6 - https://doi.org/10.3389/fmicb.2018.01368 SN - 1664-302X VL - 9 PB - Frontiers Research Foundation CY - Lausanne ER - TY - JOUR A1 - Neiber, Marco Thomas A1 - Kahl, Sandra A1 - Wiggering, Benedikt A1 - Glaubrecht, Matthias T1 - Adding the West-African riverine component BT - Revision of the Recent freshwater snails belonging to Pseudocleopatra Thiele, 1928 (Caenogastropoda, Cerithioidea, Paludomidae) JF - Zootaxa : an international journal of zootaxonomy ; a rapid international journal for animal taxonomists N2 - While Africa’s lacustrine gastropod fauna, in particular of Lake Tanganyika, has received much attention, the continent’s riverine malacofauna has long been neglected. Pseudocleopatra is a relatively poorly known paludomid gastropod genus with species found throughout the lower reaches of the West African Volta and Congo rivers. In the course of ongoing systematic revisions of African paludomids, we present here a morphometric analysis and revision of the recent species assigned to the genus, i.e., P. togoensis, P. voltana, P. dartevellei and P. bennikei, to improve taxonomic acuity for this group. We use available museum material for geometric morphometrics, multivariate ratio analysis and comparisons of radular and opercular characters. Our results demonstrate that the four recent species of Pseudocleopatra are clearly distinguishable on the basis of ratios of shell measurements as well as radular and opercular characters. Pseudocleopatra has generic-level synapomorphies including: concentric opercula with relatively large paucispiral nuclei, and rachidian radular teeth with usually 13–20 cusps. On the basis of this characterisation, the nominal species Cleopatra broecki is transferred to Pseudocleopatra. Additionally, the nominal taxon P. bennikei is synonymized with P. broecki n. comb. The phylogenetic relationships of Pseudocleopatra are currently unknown, but the observed tendency of some fossil taxa assigned to Pseudocleopatra towards thalassoidism, i.e., the resemblance to marine gastropods, has led to the hypothesis that some of the thalassoid endemics in Lake Tanganyika may have originated from or be related to Pseudocleopatra. Should this hypothesis be correct, which is in need of testing by molecular genetic methods when suitable samples become available, Pseudocleopatra may play a crucial role in understanding of the evolution of thalassoidism in African Paludomidae. KW - Africa KW - Congo River KW - Volta River KW - thalassoidism KW - geometric morphometrics KW - multivariate ratio analysis Y1 - 2019 U6 - https://doi.org/10.11646/zootaxa.4674.3.1 SN - 1175-5326 SN - 1175-5334 VL - 4674 IS - 3 SP - 301 EP - 328 PB - Magnolia Press CY - Auckland ER - TY - JOUR A1 - Kahl, Sandra A1 - Lenhard, Michael A1 - Joshi, Jasmin Radha T1 - Compensatory mechanisms to climate change in the widely distributed species Silene vulgaris JF - The journal of ecology N2 - The adaptation of plants to future climatic conditions is crucial for their survival. Not surprisingly, phenotypic responses to climate change have already been observed in many plant populations. These responses may be due to evolutionary adaptive changes or phenotypic plasticity. Especially plant species with a wide geographic range are either expected to show genetic differentiation in response to differing climate conditions or to have a high phenotypic plasticity. We investigated phenotypic responses and plasticity as an estimate of the adaptive potential in the widespread species Silene vulgaris. In a greenhouse experiment, 25 European populations covering a geographic range from the Canary Islands to Sweden were exposed to three experimental precipitation and two temperature regimes mimicking a possible climate-change scenario for central Europe. We hypothesized that southern populations have a better performance under high temperature and drought conditions, as they are already adapted to a comparable environment. We found that our treatments significantly influenced the plants, but did not reveal a latitudinal difference in response to climate treatments for most plant traits. Only flower number showed a stronger plasticity in northern European populations (e.g. Swedish populations) where numbers decreased more drastically with increased temperature and decreased precipitation treatment. Synthesis. The significant treatment response in Silene vulgaris, independent of population origin - except for the number of flowers produced - suggests a high degree of universal phenotypic plasticity in this widely distributed species. This reflects the likely adaptation strategy of the species and forms the basis for a successful survival strategy during upcoming climatic changes. However, as flower number, a strongly fitness-related trait, decreased more strongly in northern populations under a climate-change scenario, there might be limits to adaptation even in this widespread, plastic species. KW - climate change KW - global change ecology KW - latitudinal gradient KW - local adaptation KW - phenotypic plasticity KW - plant performance KW - temperature increase Y1 - 2019 U6 - https://doi.org/10.1111/1365-2745.13133 SN - 0022-0477 SN - 1365-2745 VL - 107 IS - 4 SP - 1918 EP - 1930 PB - Wiley CY - Hoboken ER - TY - GEN A1 - Zwickel, Theresa A1 - Kahl, Sandra A1 - Klaffke, Horst A1 - Rychlik, Michael A1 - Müller, Marina E. H. T1 - Spotlight on the underdogs BT - an analysis of underrepresented alternaria mycotoxins formed depending on varying substrate, time and temperature conditions N2 - Alternaria (A.) is a genus of widespread fungi capable of producing numerous, possibly health-endangering Alternaria toxins (ATs), which are usually not the focus of attention. The formation of ATs depends on the species and complex interactions of various environmental factors and is not fully understood. In this study the influence of temperature (7 °C, 25 °C), substrate (rice, wheat kernels) and incubation time (4, 7, and 14 days) on the production of thirteen ATs and three sulfoconjugated ATs by three different Alternaria isolates from the species groups A. tenuissima and A. infectoria was determined. High-performance liquid chromatography coupled with tandem mass spectrometry was used for quantification. Under nearly all conditions, tenuazonic acid was the most extensively produced toxin. At 25 °C and with increasing incubation time all toxins were formed in high amounts by the two A. tenuissima strains on both substrates with comparable mycotoxin profiles. However, for some of the toxins, stagnation or a decrease in production was observed from day 7 to 14. As opposed to the A. tenuissima strains, the A. infectoria strain only produced low amounts of ATs, but high concentrations of stemphyltoxin III. The results provide an essential insight into the quantitative in vitro AT formation under different environmental conditions, potentially transferable to different field and storage conditions T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 353 KW - Alternaria infectoria KW - A. tenuissima KW - mycotoxin profile KW - wheat KW - rice KW - Alternaria toxin sulfates KW - modified Alternaria toxins KW - altertoxins KW - altenuic acid KW - HPLC-MS/MS Y1 - 2017 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-400438 ER - TY - JOUR A1 - Romero Mujalli, Daniel A1 - Rochow, Markus A1 - Kahl, Sandra A1 - Paraskevopoulou, Sofia A1 - Folkertsma, Remco A1 - Jeltsch, Florian A1 - Tiedemann, Ralph T1 - Adaptive and nonadaptive plasticity in changing environments BT - Implications for sexual species with different life history strategies JF - Ecology and evolution N2 - Populations adapt to novel environmental conditions by genetic changes or phenotypic plasticity. Plastic responses are generally faster and can buffer fitness losses under variable conditions. Plasticity is typically modeled as random noise and linear reaction norms that assume simple one-to- one genotype–phenotype maps and no limits to the phenotypic response. Most studies on plasticity have focused on its effect on population viability. However, it is not clear, whether the advantage of plasticity depends solely on environmental fluctuations or also on the genetic and demographic properties (life histories) of populations. Here we present an individual-based model and study the relative importance of adaptive and nonadaptive plasticity for populations of sexual species with different life histories experiencing directional stochastic climate change. Environmental fluctuations were simulated using differentially autocorrelated climatic stochasticity or noise color, and scenarios of directiona climate change. Nonadaptive plasticity was simulated as a random environmental effect on trait development, while adaptive plasticity as a linear, saturating, or sinusoidal reaction norm. The last two imposed limits to the plastic response and emphasized flexible interactions of the genotype with the environment. Interestingly, this assumption led to (a) smaller phenotypic than genotypic variance in the population (many-to- one genotype–phenotype map) and the coexistence of polymorphisms, and (b) the maintenance of higher genetic variation—compared to linear reaction norms and genetic determinism—even when the population was exposed to a constant environment for several generations. Limits to plasticity led to genetic accommodation, when costs were negligible, and to the appearance of cryptic variation when limits were exceeded. We found that adaptive plasticity promoted population persistence under red environmental noise and was particularly important for life histories with low fecundity. Populations produing more offspring could cope with environmental fluctuations solely by genetic changes or random plasticity, unless environmental change was too fast. KW - developmental canalization KW - environmental change KW - genetic accommodation KW - Individual-based models KW - limits KW - many-to-one genotype–phenotype map KW - noise color KW - phenotypic plasticity KW - reaction norms KW - stochastic fluctuations Y1 - 2020 U6 - https://doi.org/10.1002/ece3.7485 SN - 2045-7758 VL - 11 IS - 11 SP - 6341 EP - 6357 PB - John Wiley & Sons, Inc. CY - New Jersey ER - TY - THES A1 - Kahl, Sandra T1 - Evolutionary adaptive responses to rapid climate change in plants T1 - Evolutionäre Anpassungsstrategien von Pflanzen an den Klimawandel BT - a case study of the widely distributed species Silene vulgaris BT - ein Fallbeispiel der weit verbreiteten Art Silene vulgaris N2 - The ongoing climate change is altering the living conditions for many organisms on this planet at an unprecedented pace. Hence, it is crucial for the survival of species to adapt to these changing conditions. In this dissertation Silene vulgaris is used as a model organism to understand the adaption strategies of widely distributed plant species to the current climate change. Especially plant species that possess a wide geographic range are expected to have a high phenotypic plasticity or to show genetic differentiation in response to the different climate conditions they grow in. However, they are often underrepresented in research. In the greenhouse experiment presented in this thesis, I examined the phenotypic responses and plasticity in S. vulgaris to estimate its’ adaptation potential. Seeds from 25 wild European populations were collected along a latitudinal gradient and grown in a greenhouse under three different precipitation (65 mm, 75 mm, 90 mm) and two different temperature regimes (18°C, 21°C) that resembled a possible climate change scenario for central Europe. Afterwards different biomass and fecundity-related plant traits were measured. The treatments significantly influenced the plants but did not reveal a latitudinal difference in response to climate treatments for most plant traits. The number of flowers per individual however, showed a stronger plasticity in northern European populations (e.g., Swedish populations) where numbers decreased more drastically with increased temperature and decreased precipitation. To gain an even deeper understanding of the adaptation of S. vulgaris to climate change it is also important to reveal the underlying phylogeny of the sampled populations. Therefore, I analysed their population genetic structure through whole genome sequencing via ddRAD. The sequencing revealed three major genetic clusters in the S. vulgaris populations sampled in Europe: one cluster comprised Southern European populations, one cluster Western European populations and another cluster contained central European populations. A following analysis of experimental trait responses among the clusters to the climate-change scenario showed that the genetic clusters significantly differed in biomass-related traits and in the days to flowering. However, half of the traits showed parallel response patterns to the experimental climate-change scenario. In addition to the potential geographic and genetic adaptation differences to climate change this dissertation also deals with the response differences between the sexes in S. vulgaris. As a gynodioecious species populations of S. vulgaris consist of female and hermaphrodite individuals and the sexes can differ in their morphological traits which is known as sexual dimorphism. As climate change is becoming an important factor influencing plant morphology it remains unclear if and how different sexes may respond in sexually dimorphic species. To examine this question the sex of each individual plant was determined during the greenhouse experiment and the measured plant traits were analysed accordingly. In general, hermaphrodites had a higher number of flowers but a lower number of leaves than females. With regards to the climate change treatment, I found that hermaphrodites showed a milder negative response to higher temperatures in the number of flowers produced and in specific leaf area (SLA) compared to females. Synthesis – The significant treatment response in Silene vulgaris, independent of population origin in most traits suggests a high degree of universal phenotypic plasticity. Also, the three European intraspecific genetic lineages detected showed comparable parallel response patterns in half of the traits suggesting considerable phenotypic plasticity. Hence, plasticity might represent a possible adaptation strategy of this widely distributed species during ongoing and future climatic changes. The results on sexual dimorphism show that females and hermaphrodites are differing mainly in their number of flowers and females are affected more strongly by the experimental climate-change scenario. These results provide a solid knowledge basis on the sexual dimorphism in S. vulgaris under climate change, but further research is needed to determine the long-term impact on the breeding system for the species. In summary this dissertation provides a comprehensive insight into the adaptation mechanisms and consequences of a widely distributed and gynodioecious plant species and leverages our understanding of the impact of anthropogenic climate change on plants. N2 - Der derzeitige Klimawandel verändert die Lebensbedingungen für viele Tiere und Pflanzen auf unserem Planeten in nie da gewesenem Maße. Damit Arten überleben, ist es von besonderer Wichtigkeit, dass sich diese an die sich ändernden Klimabedingungen anpassen können. Die vorliegende Dissertation befasst sich mit der Modellpflanze Silene vulgaris und versucht zu ergründen, wie sich solch weit verbreitete Pflanzenarten an den Klimawandel anpassen. Dabei ist zu erwarten, dass sie eine hohe phänotypische Plastizität besitzen, durch die sie sich gut anpassen können oder, dass sie sich durch eine genetische Differenzierung als Antwort auf die vorherrschenden Umweltbedingungen auszeichnen. Im experimentellen Ansatz dieser Dissertation untersuchte ich daher die phänotypischen Anpassungen und die phänotypische Plastizität von S. vulgaris an ein mögliches Klimawandelszenario für Zentraleuropa. Dabei wurden die Samen von 25 europäischen Populationen gesammelt und in einem Gewächshausexperiment unter drei verschiedenen Niederschlagsbedingungen (65 mm, 75 mm, 90 mm) und zwei verschiedenen Temperaturbedingungen (18°C, 21°C) herangezogen. Im Anschluss wurden verschiedene Biomasse- und Fertilitätsmerkmale gemessen. Für ein tiefergehendes Verständnis der Anpassungsmöglichkeiten von S. vulgaris an den Klimawandel ist es zudem wichtig, auch die zugrundeliegende Phylogenie der Populationen zu verstehen. In diesem Zusammenhang nutzte ich eine genomweite Sequenziermethode mittels ddRAD. Die Bedingungen im Gewächshausexperiment beeinflussten die Pflanzen signifikant in ihren phänotypischen Merkmalen, jedoch ließ sich kein Unterschied zwischen Population unterschiedlicher Herkunft erkennen. Lediglich die Anzahl der Blüten zeigte eine größere Plastizität in nördlichen europäischen Populationen, wo sich die Blütenzahl stärker dezimierte unter höheren Temperaturen und stärkerer Trockenheit. Die populationsgenetische Analyse ergab drei distinkte phylogenetische Gruppen für die untersuchten europäischen Populationen von S. vulgaris: eine Gruppe beinhaltete südeuropäische Populationen aus Spanien und Südfrankreich, eine weitere Gruppe bestand aus den gesammelten Individuen der westfranzösischen Populationen, während die dritte Gruppe, die Populationen aus Mittel- und Nordeuropa enthielt. Diese genetischen Gruppen wurden anschließend ebenfalls der Merkmalsanalyse unter den Gewächshausbedingungen unterzogen. Dabei stellte sich heraus, dass sich die genetischen Gruppen in ihren phänotypischen Merkmalen unterschieden, jedoch eine ähnliche Anpassung ihrer Merkmale an die experimentellen Klimawandelbedingungen zeigten. Der dritte Aspekt dieser Dissertation befasste sich mit möglichen Anpassungsunterschieden zwischen den Geschlechtern in S. vulgaris. Als gynodiözische Art bestehen ihre Populationen sowohl aus weiblichen, also auch aus zwittrigen Individuen. Die phänotypischen Merkmale beider Geschlechter können sich dabei unterscheiden, was man als Sexualdimorphismus bezeichnet. Es ist bereits bekannt, dass sich Pflanzenmerkmale durch den anhaltenden Klimawandel bereits verändern, jedoch ist es nicht gut erforscht, ob und wie sich die unterschiedlichen Geschlechter bei einer sexuell dimorphen Art unter diesem Selektionsdruck verhalten. Während des Gewächshausexperiments wurden daher die Geschlechter der Individuen bestimmt und die phänotypischen Unterschiede zwischen weiblichen und zwittrigen Pflanzen analysiert. Allgemein lässt sich sagen, dass zwittrige Individuen mehr Blüten aber weniger Blätter hatten als weibliche. Im Hinblick auf die experimentellen Klimawandelbedingungen konnte ich zudem feststellen, dass Hermaphroditen in ihrer spezifischen Blattfläche und der Blütenanzahl weniger stark negativ auf höhere Temperaturen reagierten. Synthese – Die signifikanten Merkmalsanpassungen an die Gewächshausbedingungen waren unabhängig von der geographischen Herkunft oder genetischen Gruppe der Individuen. Dies lässt ein hohes Maß an universeller, phänotypischer Plastizität vermuten. Dementsprechend kann davon ausgegangen werden, dass phänotypische Plastizität ein möglicher Anpassungsmechanismus für diese weit verbreitete Art an den Klimawandel sein könnte. Im Hinblick auf den Sexualdimorphismus in S. vulgaris lässt sich sagen, dass sich beide Geschlechter vornehmlich in der Anzahl der Blüten unterscheiden und dass weibliche Pflanzen stärker von den Bedingungen des Gewächshausexperiments beeinflusst wurden. Diese Dissertation konnte damit erstmals darüber Aufschluss geben, wie sich S. vulgaris im Hinblick auf ihren Sexualdimorphismus unter Klimawandelbedingungen verhält. Weitere Forschung wird nun benötigt, um auch den Langzeiteffekt des Klimawandels auf das Fortpflanzungssystem dieser Art abschätzen zu können. Zusammenfassend lässt sich sagen, dass die vorliegende Arbeit einen umfassenden Einblick in die Anpassungsmechanismen einer weit verbreiteten Pflanzenart an den anthropogenen Klimawandel gibt. Zudem bestärkt sie unser Verständnis der Auswirkungen, die sich daraus für eine gynodiözische Art, wie S. vulgaris ergeben. KW - Silene vulgaris KW - climate change KW - plant adaptation KW - Silene vulgaris KW - Klimawandel KW - Pflanzenanpassung Y1 - 2022 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-556483 ER - TY - GEN A1 - Zwickel, Theresa A1 - Kahl, Sandra A1 - Rychlik, Michael A1 - Müller, Marina E. H. T1 - Chemotaxonomy of mycotoxigenic small-spored Alternaria fungi BT - do multitoxin mixtures act as an indicator for species differentiation? T2 - Postprints der Universität Potsdam Mathematisch-Naturwissenschaftliche Reihe N2 - Necrotrophic as well as saprophytic small-spored Altemaria (A.) species are annually responsible for major losses of agricultural products, such as cereal crops, associated with the contamination of food and feedstuff with potential health-endangering Altemaria toxins. Knowledge of the metabolic capabilities of different species-groups to form mycotoxins is of importance for a reliable risk assessment. 93 Altemaria strains belonging to the four species groups Alternaria tenuissima, A. arborescens, A. altemata, and A. infectoria were isolated from winter wheat kernels harvested from fields in Germany and Russia and incubated under equal conditions. Chemical analysis by means of an HPLC-MS/MS multi-Alternaria-toxin-method showed that 95% of all strains were able to form at least one of the targeted 17 non-host specific Altemaria toxins. Simultaneous production of up to 15 (modified) Altemaria toxins by members of the A. tenuissima, A. arborescens, A. altemata species-groups and up to seven toxins by A. infectoria strains was demonstrated. Overall tenuazonic acid was the most extensively formed mycotoxin followed by alternariol and alternariol mono methylether, whereas altertoxin I was the most frequently detected toxin. Sulfoconjugated modifications of alternariol, alternariol mono methylether, altenuisol and altenuene were frequently determined. Unknown perylene quinone derivatives were additionally detected. Strains of the species-group A. infectoria could be segregated from strains of the other three species-groups due to significantly lower toxin levels and the specific production of infectopyrone. Apart from infectopyrone, alterperylenol was also frequently produced by 95% of the A. infectoria strains. Neither by the concentration nor by the composition of the targeted Altemaria toxins a differentiation between the species-groups A. altemata, A. tenuissima and A. arborescens was possible. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 696 KW - small-spored Alternaria fungi KW - Alternaria species-groups KW - Alternaria mycotoxins KW - chemotaxonomy KW - secondary metabolite profiling KW - LC-MS/MS KW - wheat KW - perylene quinone derivatives Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-426623 SN - 1866-8372 IS - 696 ER - TY - GEN A1 - Kahl, Sandra A1 - Kappel, Christian A1 - Joshi, Jasmin Radha A1 - Lenhard, Michael T1 - Phylogeography of a widely distributed plant species reveals cryptic genetic lineages with parallel phenotypic responses to warming and drought conditions T2 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe N2 - To predict how widely distributed species will perform under future climate change, it is crucial to understand and reveal their underlying phylogenetics. However, detailed information about plant adaptation and its genetic basis and history remains scarce and especially widely distributed species receive little attention despite their putatively high adaptability. To examine the adaptation potential of a widely distributed species, we sampled the model plant Silene vulgaris across Europe. In a greenhouse experiment, we exposed the offspring of these populations to a climate change scenario for central Europe and revealed the population structure through whole-genome sequencing. Plants were grown under two temperatures (18°C and 21°C) and three precipitation regimes (65, 75, and 90 mm) to measure their response in biomass and fecundity-related traits. To reveal the population genetic structure, ddRAD sequencing was employed for a whole-genome approach. We found three major genetic clusters in S. vulgaris from Europe: one cluster comprising Southern European populations, one cluster of Western European populations, and another cluster containing central European populations. Population genetic diversity decreased with increasing latitude, and a Mantel test revealed significant correlations between FST and geographic distances as well as between genetic and environmental distances. Our trait analysis showed that the genetic clusters significantly differed in biomass-related traits and in the days to flowering. However, half of the traits showed parallel response patterns to the experimental climate change scenario. Due to the differentiated but parallel response patterns, we assume that phenotypic plasticity plays an important role for the adaptation of the widely distributed species S. vulgaris and its intraspecific genetic lineages. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1218 KW - climate adaptation KW - ddRAD KW - Silene vulgaris Y1 - 2021 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-530035 SN - 1866-8372 SP - 13986 EP - 14002 ER - TY - GEN A1 - Romero-Mujalli, Daniel A1 - Rochow, Markus A1 - Kahl, Sandra A1 - Paraskevopoulou, Sofia A1 - Folkertsma, Remco A1 - Jeltsch, Florian A1 - Tiedemann, Ralph T1 - Adaptive and nonadaptive plasticity in changing environments: Implications for sexual species with different life history strategies T2 - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe N2 - Populations adapt to novel environmental conditions by genetic changes or phenotypic plasticity. Plastic responses are generally faster and can buffer fitness losses under variable conditions. Plasticity is typically modeled as random noise and linear reaction norms that assume simple one-to- one genotype–phenotype maps and no limits to the phenotypic response. Most studies on plasticity have focused on its effect on population viability. However, it is not clear, whether the advantage of plasticity depends solely on environmental fluctuations or also on the genetic and demographic properties (life histories) of populations. Here we present an individual-based model and study the relative importance of adaptive and nonadaptive plasticity for populations of sexual species with different life histories experiencing directional stochastic climate change. Environmental fluctuations were simulated using differentially autocorrelated climatic stochasticity or noise color, and scenarios of directiona climate change. Nonadaptive plasticity was simulated as a random environmental effect on trait development, while adaptive plasticity as a linear, saturating, or sinusoidal reaction norm. The last two imposed limits to the plastic response and emphasized flexible interactions of the genotype with the environment. Interestingly, this assumption led to (a) smaller phenotypic than genotypic variance in the population (many-to- one genotype–phenotype map) and the coexistence of polymorphisms, and (b) the maintenance of higher genetic variation—compared to linear reaction norms and genetic determinism—even when the population was exposed to a constant environment for several generations. Limits to plasticity led to genetic accommodation, when costs were negligible, and to the appearance of cryptic variation when limits were exceeded. We found that adaptive plasticity promoted population persistence under red environmental noise and was particularly important for life histories with low fecundity. Populations produing more offspring could cope with environmental fluctuations solely by genetic changes or random plasticity, unless environmental change was too fast. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1170 KW - developmental canalization KW - environmental change KW - genetic accommodation KW - Individual-based models KW - limits KW - many-to-one genotype–phenotype map KW - noise color KW - phenotypic plasticity KW - reaction norms KW - stochastic fluctuations Y1 - 2020 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-523201 SN - 1866-8372 IS - 1170 ER - TY - JOUR A1 - Zwickel, Theresa A1 - Kahl, Sandra A1 - Klaffke, Horst A1 - Rychlik, Michael A1 - Müller, Marina E. H. T1 - Spotlight on the Underdogs-An Analysis of Underrepresented Alternaria Mycotoxins Formed Depending on Varying Substrate, Time and Temperature Conditions JF - Toxins N2 - Alternaria (A.) is a genus of widespread fungi capable of producing numerous, possibly health-endangering Alternaria toxins (ATs), which are usually not the focus of attention. The formation of ATs depends on the species and complex interactions of various environmental factors and is not fully understood. In this study the influence of temperature (7 degrees C, 25 degrees C), substrate (rice, wheat kernels) and incubation time (4, 7, and 14 days) on the production of thirteen ATs and three sulfoconjugated ATs by three different Alternaria isolates from the species groups A. tenuissima and A. infectoria was determined. High-performance liquid chromatography coupled with tandem mass spectrometry was used for quantification. Under nearly all conditions, tenuazonic acid was the most extensively produced toxin. At 25 degrees C and with increasing incubation time all toxins were formed in high amounts by the two A. tenuissima strains on both substrates with comparable mycotoxin profiles. However, for some of the toxins, stagnation or a decrease in production was observed from day 7 to 14. As opposed to the A. tenuissima strains, the A. infectoria strain only produced low amounts of ATs, but high concentrations of stemphyltoxin III. The results provide an essential insight into the quantitative in vitro AT formation under different environmental conditions, potentially transferable to different field and storage conditions. KW - Alternaria infectoria KW - A. tenuissima KW - mycotoxin profile KW - wheat KW - rice KW - Alternaria toxin sulfates KW - modified Alternaria toxins KW - altertoxins KW - altenuic acid KW - HPLC-MS/MS Y1 - 2016 U6 - https://doi.org/10.3390/toxins8110344 SN - 2072-6651 VL - 8 SP - 570 EP - 583 PB - MDPI CY - Basel ER -