TY  - JOUR
A1  - de la Cruz, Jorge Gonzalez
A1  - Machens, Fabian
A1  - Messerschmidt, Katrin
A1  - Bar-Even, Arren
T1  - Core Catalysis of the Reductive Glycine Pathway Demonstrated in Yeast
JF  - ACS synthetic biology
N2  - One-carbon (C1) compounds are attractive microbial feedstocks as they can be efficiently produced from widely available resources. Formate, in particular, represents a promising growth substrate, as it can be generated from electrochemical reduction of CO2 and fed to microorganisms in a soluble form. We previously identified the synthetic reductive glycine pathway as the most efficient route for aerobic growth on formate. We further demonstrated pathway activity in Escherichia coli after expression of both native and foreign genes. Here, we explore whether the reductive glycine pathway could be established in a model microorganism using only native enzymes. We used the yeast Saccharomyces cerevisiae as host and show that overexpression of only endogenous enzymes enables glycine biosynthesis from formate and CO2 in a strain that is otherwise auxotrophic for glycine. We find the pathway to be highly active in this host, where 0.125 mM formate is sufficient to support growth. Notably, the formate-dependent growth rate of the engineered S. cerevisiae strain remained roughly constant over a very wide range of formate concentrations, 1-500 mM, indicating both high affinity for formate use and high tolerance toward elevated concentration of this C1 feedstock. Our results, as well the availability of endogenous NAD-dependent formate dehydrogenase, indicate that yeast might be an especially suitable host for engineering growth on formate.
KW  - metabolic engineering
KW  - synthetic biology
KW  - one-carbon metabolism
KW  - carbon labeling
KW  - tetrahydrofolate
KW  - glycine cleavage system
Y1  - 2019
U6  - https://doi.org/10.1021/acssynbio.8b00464
SN  - 2161-5063
VL  - 8
IS  - 5
SP  - 911
EP  - 917
PB  - American Chemical Society
CY  - Washington
ER  - 
TY  - THES
A1  - Gonzalez de la Cruz, Jorge
T1  - Metabolic engineering of Saccharomyces cerevisiae for formatotrophic growth
Y1  - 2019
ER  - 
TY  - CHAP
A1  - González, Jorge
A1  - Casacuberta, Francisco
T1  - Phrase-based finite state models
N2  - In the last years, statistical machine translation has already demonstrated its usefulness within a wide variety of translation applications. In this line, phrase-based alignment models have become the reference to follow in order to build competitive systems. Finite state models are always an interesting framework because there are well-known efficient algorithms for their representation and manipulation. This document is a contribution to the evolution of finite state models towards a phrase-based approach. The inference of stochastic transducers that are based on bilingual phrases is carefully analysed from a finite state point of view. Indeed, the algorithmic phenomena that have to be taken into account in order to deal with such phrase-based finite state models when in decoding time are also in-depth detailed.
Y1  - 2008
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-27209
ER  - 
TY  - JOUR
A1  - Gonzalez-Chavarria, Ivan
A1  - Duprat, Felix
A1  - Roa, Francisco J.
A1  - Jara, Nery
A1  - Toledo, Jorge R.
A1  - Miranda, Felipe
A1  - Becerra, Jose
A1  - Inostroza, Alejandro
A1  - Kelling, Alexandra
A1  - Schilde, Uwe
A1  - Heydenreich, Matthias
A1  - Paz, Cristian
T1  - Maytenus disticha extract and an isolated β-Dihydroagarofuran induce mitochondrial depolarization and apoptosis in human cancer cells by increasing mitochondrial reactive oxygen species
JF  - Biomolecules
N2  - Maytenus disticha (Hook F.), belonging to the Celastraceae family, is an evergreen shrub, native of the central southern mountains of Chile. Previous studies demonstrated that the total extract of M. disticha (MD) has an acetylcholinesterase inhibitory activity along with growth regulatory and insecticidal activities. beta-Dihydroagarofurans sesquiterpenes are the most active components in the plant. However, its activity in cancer has not been analyzed yet. Here, we demonstrate that MD has a cytotoxic activity on breast (MCF-7), lung (PC9), and prostate (C4-2B) human cancer cells with an IC50 (mu g/mL) of 40, 4.7, and 5 mu g/mL, respectively, an increasing Bax/Bcl2 ratio, and inducing a mitochondrial membrane depolarization. The beta-dihydroagarofuran-type sesquiterpene (MD-6), dihydromyricetin (MD-9), and dihydromyricetin-3-O-beta-glucoside (MD-10) were isolated as the major compounds from MD extracts. From these compounds, only MD-6 showed cytotoxic activity on MCF-7, PC9, and C4-2B with an IC50 of 31.02, 17.58, and 42.19 mu M, respectively. Furthermore, the MD-6 increases cell ROS generation, and MD and MD-6 induce a mitochondrial superoxide generation and apoptosis on MCF-7, PC9, and C4-2B, which suggests that the cytotoxic effect of MD is mediated in part by the beta-dihydroagarofuran-type that induces apoptosis by a mitochondrial dysfunction.
KW  - Maytenus disticha
KW  - beta-dihydroagarofuran-type sesquiterpene
KW  - dihydromyricetin
KW  - dihydromyricetin-3-O-beta-glucoside
KW  - cytotoxic
KW  - activity
KW  - Mitochondrial ROS
Y1  - 2020
U6  - https://doi.org/10.3390/biom10030377
SN  - 2218-273X
VL  - 10
IS  - 3
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Hofman, Maarten P. G.
A1  - Hayward, M. W.
A1  - Heim, M.
A1  - Marchand, P.
A1  - Rolandsen, C. M.
A1  - Mattisson, Jenny
A1  - Urbano, F.
A1  - Heurich, M.
A1  - Mysterud, A.
A1  - Melzheimer, J.
A1  - Morellet, N.
A1  - Voigt, Ulrich
A1  - Allen, B. L.
A1  - Gehr, Benedikt
A1  - Rouco Zufiaurre, Carlos
A1  - Ullmann, Wiebke
A1  - Holand, O.
A1  - Jorgensen, n H.
A1  - Steinheim, G.
A1  - Cagnacci, F.
A1  - Kroeschel, M.
A1  - Kaczensky, P.
A1  - Buuveibaatar, B.
A1  - Payne, J. C.
A1  - Palmegiani, I
A1  - Jerina, K.
A1  - Kjellander, P.
A1  - Johansson, O.
A1  - LaPoint, S.
A1  - Bayrakcismith, R.
A1  - Linnell, J. D. C.
A1  - Zaccaroni, M.
A1  - Jorge, M. L. S.
A1  - Oshima, J. E. F.
A1  - Songhurst, A.
A1  - Fischer, C.
A1  - Mc Bride, R. T.
A1  - Thompson, J. J.
A1  - Streif, S.
A1  - Sandfort, R.
A1  - Bonenfant, Christophe
A1  - Drouilly, M.
A1  - Klapproth, M.
A1  - Zinner, Dietmar
A1  - Yarnell, Richard
A1  - Stronza, A.
A1  - Wilmott, L.
A1  - Meisingset, E.
A1  - Thaker, Maria
A1  - Vanak, A. T.
A1  - Nicoloso, S.
A1  - Graeber, R.
A1  - Said, S.
A1  - Boudreau, M. R.
A1  - Devlin, A.
A1  - Hoogesteijn, R.
A1  - May-Junior, J. A.
A1  - Nifong, J. C.
A1  - Odden, J.
A1  - Quigley, H. B.
A1  - Tortato, F.
A1  - Parker, D. M.
A1  - Caso, A.
A1  - Perrine, J.
A1  - Tellaeche, C.
A1  - Zieba, F.
A1  - Zwijacz-Kozica, T.
A1  - Appel, C. L.
A1  - Axsom, I
A1  - Bean, W. T.
A1  - Cristescu, B.
A1  - Periquet, S.
A1  - Teichman, K. J.
A1  - Karpanty, S.
A1  - Licoppe, A.
A1  - Menges, V
A1  - Black, K.
A1  - Scheppers, Thomas L.
A1  - Schai-Braun, S. C.
A1  - Azevedo, F. C.
A1  - Lemos, F. G.
A1  - Payne, A.
A1  - Swanepoel, L. H.
A1  - Weckworth, B.
A1  - Berger, A.
A1  - Bertassoni, Alessandra
A1  - McCulloch, G.
A1  - Sustr, P.
A1  - Athreya, V
A1  - Bockmuhl, D.
A1  - Casaer, J.
A1  - Ekori, A.
A1  - Melovski, D.
A1  - Richard-Hansen, C.
A1  - van de Vyver, D.
A1  - Reyna-Hurtado, R.
A1  - Robardet, E.
A1  - Selva, N.
A1  - Sergiel, A.
A1  - Farhadinia, M. S.
A1  - Sunde, P.
A1  - Portas, R.
A1  - Ambarli, Hüseyin
A1  - Berzins, R.
A1  - Kappeler, P. M.
A1  - Mann, G. K.
A1  - Pyritz, L.
A1  - Bissett, C.
A1  - Grant, T.
A1  - Steinmetz, R.
A1  - Swedell, Larissa
A1  - Welch, R. J.
A1  - Armenteras, D.
A1  - Bidder, O. R.
A1  - Gonzalez, T. M.
A1  - Rosenblatt, A.
A1  - Kachel, S.
A1  - Balkenhol, N.
T1  - Right on track?
BT  - Performance of satellite telemetry in terrestrial wildlife research
JF  - PLoS one
N2  - Satellite telemetry is an increasingly utilized technology in wildlife research, and current devices can track individual animal movements at unprecedented spatial and temporal resolutions. However, as we enter the golden age of satellite telemetry, we need an in-depth understanding of the main technological, species-specific and environmental factors that determine the success and failure of satellite tracking devices across species and habitats. Here, we assess the relative influence of such factors on the ability of satellite telemetry units to provide the expected amount and quality of data by analyzing data from over 3,000 devices deployed on 62 terrestrial species in 167 projects worldwide. We evaluate the success rate in obtaining GPS fixes as well as in transferring these fixes to the user and we evaluate failure rates. Average fix success and data transfer rates were high and were generally better predicted by species and unit characteristics, while environmental characteristics influenced the variability of performance. However, 48% of the unit deployments ended prematurely, half of them due to technical failure. Nonetheless, this study shows that the performance of satellite telemetry applications has shown improvements over time, and based on our findings, we provide further recommendations for both users and manufacturers.
Y1  - 2019
U6  - https://doi.org/10.1371/journal.pone.0216223
SN  - 1932-6203
VL  - 14
IS  - 5
PB  - PLoS
CY  - San Fransisco
ER  -