TY - JOUR A1 - Zhang, Houbin A1 - Hanke-Gogokhia, Christin A1 - Jiang, Li A1 - Li, Xiaobo A1 - Wang, Pu A1 - Gerstner, Cecilia D. A1 - Frederick, Jeanne M. A1 - Yang, Zhenglin A1 - Baehr, Wolfgang T1 - Mistrafficking of prenylated proteins causes retinitis pigmentosa 2 JF - The FASEB journal : the official journal of the Federation of American Societies for Experimental Biology N2 - The retinitis pigmentosa 2 polypeptide (RP2) functions as a GTPase-activating protein (GAP) for ARL3 (Arf-like protein 3), a small GTPase. ARL3 is an effector of phosphodiesterase 6 Delta (PDE6D), a prenyl-binding protein and chaperone of prenylated protein in photoreceptors. Mutations in the human RP2 gene cause X-linked retinitis pigmentosa (XLRP) and cone-rod dystrophy (XL-CORD). To study mechanisms causing XLRP, we generated an RP2 knockout mouse. The RP2h(-/-) mice exhibited a slowly progressing rod-cone dystrophy simulating the human disease. RP2h(-/-) scotopic a-wave and photopic b-wave amplitudes declined at 1 mo of age and continued to decline over the next 6 mo. Prenylated PDE6 subunits and G-protein coupled receptor kinase 1 (GRK1) were unable to traffic effectively to the RP2h(-/-) outer segments. Mechanistically, absence of RP2 GAP activity increases ARL3-GTP levels, forcing PDE6D to assume a predominantly "closed" conformation that impedes binding of lipids. Lack of interaction disrupts trafficking of PDE6 and GRK1 to their destination, the photoreceptor outer segments. We propose that hyperactivity of ARL3-GTP in RP2 knockout mice and human patients with RP2 null alleles leads to XLRP resembling recessive rod-cone dystrophy. KW - rod-cone dystrophy KW - ARL3 KW - PDE6D KW - RP2 KW - XLRP Y1 - 2015 U6 - https://doi.org/10.1096/fj.14-257915 SN - 0892-6638 SN - 1530-6860 VL - 29 IS - 3 SP - 932 EP - 942 PB - Federation of American Societies for Experimental Biology CY - Bethesda ER - TY - BOOK A1 - Chen, Huan A1 - Li, Wei-Xi A1 - Xu, Chao-Jiang T1 - Gevrey hypoellipticity for linear and non-linear fokker-planck equations T3 - Preprint / Universität Potsdam, Institut für Mathematik, Arbeitsgruppe Partiell Y1 - 2007 SN - 1437-739X PB - Univ. CY - Potsdam ER - TY - JOUR A1 - Yang, Guang A1 - Zheng, Wei A1 - Tao, Guoqing A1 - Wu, Libin A1 - Zhou, Qi-Feng A1 - Kochovski, Zdravko A1 - Ji, Tan A1 - Chen, Huaijun A1 - Li, Xiaopeng A1 - Lu, Yan A1 - Ding, Hong-ming A1 - Yang, Hai-Bo A1 - Chen, Guosong A1 - Jiang, Ming T1 - Diversiform and Transformable Glyco-Nanostructures Constructed from Amphiphilic Supramolecular Metallocarbohydrates through Hierarchical Self-Assembly: The Balance between Metallacycles and Saccharides JF - ACS nano N2 - During the past decade, self-assembly of saccharide-containing amphiphilic molecules toward bioinspired functional glycomaterials has attracted continuous attention due to their various applications in fundamental and practical areas. However, it still remains a great challenge to prepare hierarchical glycoassemblies with controllable and diversiform structures because of the complexity of saccharide structures and carbohydrate-carbohydrate interactions. Herein, through hierarchical self-assembly of modulated amphiphilic supramolecular metallocarbohydrates, we successfully prepared various well-defined glyco-nanostructures in aqueous solution, including vesicles, solid spheres, and opened vesicles depending on the molecular structures of metallocarbohydrates. More attractively, these glyco-nanostructures can further transform into other morphological structures in aqueous solutions such as worm-like micelles, tubules, and even tupanvirus-like vesicles (TVVs). It is worth mentioning that distinctive anisotropic structures including the opened vesicles (OVs) and TVVs were rarely reported in glycobased nano-objects. This intriguing diversity was mainly controlled by the subtle structural trade-off of the two major components of the amphiphiles, i.e., the saccharides and metallacycles. To further understand this precise structural control, molecular simulations provided deep physical insights on the morphology evolution and balancing of the contributions from saccharides and metallacycles. Moreover, the multivalency of glyco-nanostructures with different shapes and sizes was demonstrated by agglutination with a diversity of sugarbinding protein receptors such as the plant lectins Concanavalin A (ConA). This modular synthesis strategy provides access to systematic tuning of molecular structure and self-assembled architecture, which undoubtedly will broaden our horizons on the controllable fabrication of biomimetic glycomaterials such as biological membranes and supramolecular lectin inhibitors. KW - glycomaterials KW - diversiform structures KW - hierarchical self-assembly KW - metallocarbohydrates KW - anisotropic structures Y1 - 2019 U6 - https://doi.org/10.1021/acsnano.9b07134 SN - 1936-0851 SN - 1936-086X VL - 13 IS - 11 SP - 13474 EP - 13485 PB - American Chemical Society CY - Washington ER - TY - JOUR A1 - Wang, Li A1 - Razzaq, Muhammad Yasar A1 - Rudolph, Tobias A1 - Heuchel, Matthias A1 - Nöchel, Ulrich A1 - Mansfeld, Ulrich A1 - Jiang, Yi A1 - Gould, Oliver E. C. A1 - Behl, Marc A1 - Kratz, Karl A1 - Lendlein, Andreas T1 - Reprogrammable, magnetically controlled polymeric nanocomposite actuators JF - Material horizons N2 - Soft robots and devices with the advanced capability to perform adaptive motions similar to that of human beings often have stimuli-sensitive polymeric materials as the key actuating component. The external signals triggering the smart polymers’ actuations can be transmitted either via a direct physical connection between actuator and controlling unit (tethered) or remotely without a connecting wire. However, the vast majority of such polymeric actuator materials are limited to one specific type of motion as their geometrical information is chemically fixed. Here, we present magnetically driven nanocomposite actuators, which can be reversibly reprogrammed to different actuation geometries by a solely physical procedure. Our approach is based on nanocomposite materials comprising spatially segregated crystallizable actuation and geometry determining units. Upon exposure to a specific magnetic field strength the actuators’ geometric memory is erased by the melting of the geometry determining units allowing the implementation of a new actuator shape. The actuation performance of the nanocomposites can be tuned and the technical significance was demonstrated in a multi-cyclic experiment with several hundreds of repetitive free-standing shape shifts without losing performance. Y1 - 2018 U6 - https://doi.org/10.1039/c8mh00266e SN - 2051-6347 SN - 2051-6355 VL - 5 IS - 5 SP - 861 EP - 867 PB - Royal Society of Chemistry CY - Cambridge ER - TY - INPR A1 - Chen, Hua A1 - Li, Wei-Xi A1 - Xu, Chao-Jiang T1 - Gevrey hypoellipticity for linear and non-linear Fokker-Planck equations N2 - This paper studies the Gevrey regularity of weak solutions of a class of linear and semilinear Fokker-Planck equations. T3 - Preprint - (2007) 07 Y1 - 2007 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-30283 ER - TY - JOUR A1 - Jia, Weihan A1 - Anslan, Sten A1 - Chen, Fahu A1 - Cao, Xianyong A1 - Dong, Hailiang A1 - Dulias, Katharina A1 - Gu, Zhengquan A1 - Heinecke, Liv A1 - Jiang, Hongchen A1 - Kruse, Stefan A1 - Kang, Wengang A1 - Li, Kai A1 - Liu, Sisi A1 - Liu, Xingqi A1 - Liu, Ying A1 - Ni, Jian A1 - Schwalb, Antje A1 - Stoof-Leichsenring, Kathleen R. A1 - Shen, Wei A1 - Tian, Fang A1 - Wang, Jing A1 - Wang, Yongbo A1 - Wang, Yucheng A1 - Xu, Hai A1 - Yang, Xiaoyan A1 - Zhang, Dongju A1 - Herzschuh, Ulrike T1 - Sedimentary ancient DNA reveals past ecosystem and biodiversity changes on the Tibetan Plateau: overview and prospects JF - Quaternary science reviews : the international multidisciplinary research and review journal N2 - Alpine ecosystems on the Tibetan Plateau are being threatened by ongoing climate warming and intensified human activities. Ecological time-series obtained from sedimentary ancient DNA (sedaDNA) are essential for understanding past ecosystem and biodiversity dynamics on the Tibetan Plateau and their responses to climate change at a high taxonomic resolution. Hitherto only few but promising studies have been published on this topic. The potential and limitations of using sedaDNA on the Tibetan Plateau are not fully understood. Here, we (i) provide updated knowledge of and a brief introduction to the suitable archives, region-specific taphonomy, state-of-the-art methodologies, and research questions of sedaDNA on the Tibetan Plateau; (ii) review published and ongoing sedaDNA studies from the Tibetan Plateau; and (iii) give some recommendations for future sedaDNA study designs. Based on the current knowledge of taphonomy, we infer that deep glacial lakes with freshwater and high clay sediment input, such as those from the southern and southeastern Tibetan Plateau, may have a high potential for sedaDNA studies. Metabarcoding (for microorganisms and plants), metagenomics (for ecosystems), and hybridization capture (for prehistoric humans) are three primary sedaDNA approaches which have been successfully applied on the Tibetan Plateau, but their power is still limited by several technical issues, such as PCR bias and incompleteness of taxonomic reference databases. Setting up high-quality and open-access regional taxonomic reference databases for the Tibetan Plateau should be given priority in the future. To conclude, the archival, taphonomic, and methodological conditions of the Tibetan Plateau are favorable for performing sedaDNA studies. More research should be encouraged to address questions about long-term ecological dynamics at ecosystem scale and to bring the paleoecology of the Tibetan Plateau into a new era. KW - Sedimentary ancient DNA (sedaDNA) KW - Tibetan Plateau KW - Environmental DNA KW - Taphonomy KW - Ecosystem KW - Biodiversity KW - Paleoecology KW - Paleogeography Y1 - 2022 U6 - https://doi.org/10.1016/j.quascirev.2022.107703 SN - 0277-3791 SN - 1873-457X VL - 293 PB - Elsevier CY - Oxford ER - TY - JOUR A1 - Kappel, Christian A1 - Friedrich, Thomas A1 - Oberkofler, Vicky A1 - Jiang, Li A1 - Crawford, Tim A1 - Lenhard, Michael A1 - Bäurle, Isabel T1 - Genomic and epigenomic determinants of heat stress-induced transcriptional memory in Arabidopsis JF - Genome biology : biology for the post-genomic era N2 - Background Transcriptional regulation is a key aspect of environmental stress responses. Heat stress induces transcriptional memory, i.e., sustained induction or enhanced re-induction of transcription, that allows plants to respond more efficiently to a recurrent HS. In light of more frequent temperature extremes due to climate change, improving heat tolerance in crop plants is an important breeding goal. However, not all heat stress-inducible genes show transcriptional memory, and it is unclear what distinguishes memory from non-memory genes. To address this issue and understand the genome and epigenome architecture of transcriptional memory after heat stress, we identify the global target genes of two key memory heat shock transcription factors, HSFA2 and HSFA3, using time course ChIP-seq. Results HSFA2 and HSFA3 show near identical binding patterns. In vitro and in vivo binding strength is highly correlated, indicating the importance of DNA sequence elements. In particular, genes with transcriptional memory are strongly enriched for a tripartite heat shock element, and are hallmarked by several features: low expression levels in the absence of heat stress, accessible chromatin environment, and heat stress-induced enrichment of H3K4 trimethylation. These results are confirmed by an orthogonal transcriptomic data set using both de novo clustering and an established definition of memory genes. Conclusions Our findings provide an integrated view of HSF-dependent transcriptional memory and shed light on its sequence and chromatin determinants, enabling the prediction and engineering of genes with transcriptional memory behavior. KW - Transcriptional memory KW - Priming KW - Heat stress KW - HSFA2 KW - HSFA3 KW - Arabidopsis thaliana KW - Histone H3K4 trimethylation KW - ChIP-seq Y1 - 2023 U6 - https://doi.org/10.1186/s13059-023-02970-5 SN - 1474-760X VL - 24 IS - 1 PB - BioMed Central CY - London ER - TY - THES A1 - Jiang, Li T1 - Analysis of the role of Heat shock factors and Mediator subunits in heat stress memory in Arabidopsis thaliana N2 - In nature, plants often encounter biotic and abiotic stresses, which can cause reduced crop yield and quality, and threaten the nutrition of a growing human population. As heat stress (HS) is one of the main abiotic stresses, and is projected to increase due to global warming, it is necessary to better understand how plants respond and survive under HS. In Arabidopsis thaliana, plants can survive under severe HS if primed by a non-lethal HS, a process called acquisition of thermotolerance. This primed stated can be maintained for several days, and the ability of plants to maintain the primed state is called maintenance of acquired thermotolerance (mATT) or HS memory. According to current research, two Heat shock factors (HSFs) HSFA2 and HSFA3 are known to account for the majority of mATT capability, and there are other HSFs e.g. HSFA1b and HSFA6b in HSF complexes containing HSFA2 and/or HSFA3, however, the roles of these HSFs in HS memory is not clearly understood. Moreover, the mechanism of these HSFs in regulating HS memory is unclear, whether transcriptional machinery e.g. the Mediator complex contributes to transcriptional memory. This work investigates the role of HSFs and Mediator subunits in HS memory in A. thaliana. For the role of HSFs, the interaction between HSFA1b and HSFA2 during HS memory phase was confirmed by in vivo co- immunoprecipitation (Co-IP). HSFA1b, HSFA2, HSFA3 and HSFA6b targeted HS memory-related genes according to DNA affinity purification sequencing (DAP-seq) data, and targets of HSFA1b were confirmed in vivo by chromatin immunoprecipitation qPCR (ChIP-qPCR). The mutant of hsfa6b showed an HS memory deficiency phenotype in mATT survival assay. These data confirmed the role for HSFA2 and HSFA3 in HS memory, and suggest that HSFA1b and HSFA6b also function in HS memory. The Mediator complex functions as an RNA Polymerase II (RNA Pol II) co-regulator, and includes Head, Middle, Tail and Kinase modules. Both MED23 and MED32 belong to the Tail module, and they have a positive role in HS memory. MED23 interacted with HSFA3, as determined by yeast two hybrid (Y2H) and in vivo Co-IP assays. The med23 mutant showed a decreased HS memory phenotype, reduced expression of Type I (sustained expression) memory genes following HS, and reduced accumulation of the memory-associated Tri-methylation of histone H3 lysine 4 (H3K4me3)histone modification at HS memory-related gene loci after HS. MED23 was recruited to HS-inducible memory and non-memory genes after HS, as determined by ChIP-qPCR. The med32 mutant showed a reduced HS memory phenotype, decreased expression of Type I and Type II (hyper-induction) memory genes, and lower accumulation of H3K4me3 at memory gene lociafter HS. However, MED32 did not show interaction with any tested HSF in Y2H or in vivo Co-IP. MED32 regulated the recruitment of RNA Pol II at HS-inducible genes after HS, but was not itself recruited to HS memory genes after HS. These results provided more evidence that the Mediator subunits MED23 and MED32 regulate HS memory on transcriptional and epigenetic levels. In general, this work provides a better insight into the molecular mechanism of how HSFs and Mediator subunits regulate HS memory in plants and will provide new perspectives to breed crops with improved thermotolerance. N2 - In der Natur sind Pflanzen häufig mit biotischen und abiotischen Stressfaktoren konfrontiert, die zu Ertrags- und Qualitätsmängeln führen können und die Ernährung der wachsenden Weltbevölkerung gefährden. Da Hitzestress (HS) einer der wichtigsten abiotischen Stressfaktoren ist und aufgrund der globalen Erwärmung voraussichtlich noch zunehmen wird, steigt die Notwendigkeit zu verstehen wie Pflanzen auf HS reagieren und überleben. Arabidopsis thaliana können unter schwerem Hitzestress überleben, wenn sie durch einen nicht-tödlichen Hitzestress vorbereitet werden, diesen Prozess bezeichnet man als Erwerb von Thermotoleranz. Die Fähigkeit der Pflanzen, diesen Zustand aufrechtzuerhalten, wird als Aufrechterhaltung der erworbenen Thermotoleranz (mATT) oder HS-Gedächtnis bezeichnet. Nach dem derzeitigen Stand der Forschung sind zwei Hitzeschockfaktoren (HSFs), HSFA2 und HSFA3, für den Großteil der mATT-Fähigkeit verantwortlich. Außerdem gibt es weitere HSFs z.B. HSFA1b und HSFA6b, die ebenfalls im HSF-Komplex neben HSFA2 und/oder HSFA3 enthalten sind. Jedoch ist ihre Rolle im HS-Gedächtnis noch nicht eindeutig geklärt. Darüber hinaus ist der Mechanismus dieser HSFs bei der Regulierung des HS-Gedächtnisses unklar, ob z. B. die Transkriptionsmaschinerie, unter anderem der Mediator-Komplex, zum Transkriptionsgedächtnis beiträgt. In dieser Arbeit wird die Rolle der HSFs und der Mediator-Untereinheiten beim HS-Gedächtnis in A. thaliana untersucht. Bezüglich der Rolle der HSFs, wurde die Interaktion zwischen HSFA1b und HSFA2, während der HS-Gedächtnisphase, durch in vivo Co-Immunopräzipitation (Co-IP) bestätigt. HSFA1b, HSFA2, HSFA3 und HSFA6b binden, laut DNA-Affinitätsreinigungs-Sequenzierung (DAP-seq), zielgerichtet an HS-Gedächtnis-verwandte Gene. Die Ziele von HSFA1b wurden in vivo durch Chromatin-Immunpräzipitation qPCR (ChIP-qPCR) bestätigt. Die hsfa6b-Mutante zeigte im mATT-Überlebenstest einen Phänotypen für HS-Gedächtnisdefizit. Diese Daten bestätigten die Rolle von HSFA2 und HSFA3 im HS-Gedächtnis und legen nahe, dass HSFA1b und HSFA6b ebenfalls eine Funktion im HS-Gedächtnis haben. Der Mediator-Komplex fungiert als Co-Regulator der RNA-Polymerase II (RNA Pol II) und umfasst die Module Head, Middle, Tail und Kinase. Sowohl MED23 als auch MED32 gehören zum Tail-Modul und spielen eine positive Rolle im HS-Gedächtnis. MED23 interagiert mit HSFA3, wie durch Hefe-Zwei-Hybrid- (Y2H) und In-vivo-Co-IP-Assays festgestellt wurde. Die med23-Mutante zeigte einen verminderten HS-Gedächtnisphänotyp, eine verringerte Expression von Gedächtnisgenen des Typ I (anhaltende Expression) nach HS und eine verringerte Akkumulation der gedächtnisassoziierten Histonmodifikation Tri-Methylierung von Histon H3 Lysin 4 (H3K4me3) an HS-Gedächtnis-bezogenen Genorten nach HS. MED23 wurde nach HS an HS-induzierbaren Gedächtnis- und Nicht-Gedächtnis-Genen rekrutiert, wie durch ChIP-qPCR festgestellt wurde. Die med32-Mutante zeigte einen reduzierten HS-Gedächtnisphänotyp, eine verringerte Expression von Typ-I- und Typ-II-Gedächtnisgenen (Hyperinduktion) und eine geringere Anhäufung von H3K4me3 an Gedächtnisgenloci nach HS. MED32 zeigte jedoch keine Interaktion mit einem der getesteten HSFs in Y2H oder in vivo Co-IP. MED32 regulierte die Rekrutierung von RNA Pol II an HS-induzierbaren Genen nach HS, wurde aber selbst nicht an HS-Gedächtnisgenen nach HS rekrutiert. Diese Ergebnisse liefern weitere Beweise dafür, dass die Mediator-Untereinheiten MED23 und MED32 das HS-Gedächtnis auf transkriptioneller und epigenetischer Ebene regulieren. Im Allgemeinen bietet diese Arbeit einen besseren Einblick in den molekularen Mechanismus, wie HSFs und Mediator-Untereinheiten das HS-Gedächtnis in Pflanzen regulieren und wird neue Perspektiven für die Züchtung von Pflanzen mit verbesserter Thermotoleranz eröffnen. KW - Heat stress memory, Heat shock factors, Mediator subunits, MED23, MED32, Arabidopsis thaliana Y1 - 2023 ER - TY - JOUR A1 - Ronquillo, Cecinio C. A1 - Hanke-Gogokhia, Christin A1 - Revelo, Monica P. A1 - Frederick, Jeanne M. A1 - Jiang, Li A1 - Baehr, Wolfgang T1 - Ciliopathy-associated IQCB1/NPHP5 protein is required for mouse photoreceptor outer segment formation JF - The FASEB journal : the official journal of the Federation of American Societies for Experimental Biology N2 - Null mutations in the human IQCB1/NPHP5 (nephrocystin-5) gene that encodes NPHP5 are the most frequent cause of Senior-LOken syndrome, a ciliopathy that is characterized by Leber congenital amaurosis and nephronophthisis. We generated germline Nphp5-knockout mice by placing a -Geo gene trap in intron 4, thereby truncating NPHP5 at Leu87 and removing all known functional domains. At eye opening, Nphp5(-/-) mice exhibited absence of scotopic and photopic electroretinogram responses, a phenotype that resembles Leber congenital amaurosis. Outer segment transmembrane protein accumulation in Nphp5(-/-) endoplasmic reticulum was evident as early as postnatal day (P)6. EGFP-CETN2, a centrosome and transition zone marker, identified basal bodies in Nphp5(-/-) photoreceptors, but without fully developed transition zones. Ultrastructure of P6 and 10 Nphp5(-/-) photoreceptors revealed aberrant transition zones of reduced diameter. Nphp5(-/-) photoreceptor degeneration was complete at 1 mo of age but was delayed significantly in Nphp5(-/-);Nrl(-/-) (cone only) retina. Nphp5(-/-) mouse embryonic fibroblast developed normal cilia, and Nphp5(-/-) kidney histology at 1 yr of age showed no significant pathology. Results establish that nephrocystin-5 is essential for photoreceptor outer segment formation but is dispensable for kidney and mouse embryonic fibroblast ciliary formation. KW - Senior-LOken syndrome KW - nephronophthisis KW - Leber congenital amaurosis KW - nephrocystins Y1 - 2016 U6 - https://doi.org/10.1096/fj.201600511R SN - 0892-6638 SN - 1530-6860 VL - 30 SP - 3400 EP - 3412 PB - Federation of American Societies for Experimental Biology CY - Bethesda ER - TY - JOUR A1 - Cook, Katherine V. A1 - Li, Chuang A1 - Cai, Haiyuan A1 - Krumholz, Lee R. A1 - Hambright, K. David A1 - Paerl, Hans W. A1 - Steffen, Morgan M. A1 - Wilson, Alan E. A1 - Burford, Michele A. A1 - Grossart, Hans-Peter A1 - Hamilton, David P. A1 - Jiang, Helong A1 - Sukenik, Assaf A1 - Latour, Delphine A1 - Meyer, Elisabeth I. A1 - Padisak, Judit A1 - Qin, Boqiang A1 - Zamor, Richard M. A1 - Zhu, Guangwei T1 - The global Microcystis interactome JF - Limnology and oceanography N2 - Bacteria play key roles in the function and diversity of aquatic systems, but aside from study of specific bloom systems, little is known about the diversity or biogeography of bacteria associated with harmful cyanobacterial blooms (cyanoHABs). CyanoHAB species are known to shape bacterial community composition and to rely on functions provided by the associated bacteria, leading to the hypothesized cyanoHAB interactome, a coevolved community of synergistic and interacting bacteria species, each necessary for the success of the others. Here, we surveyed the microbiome associated with Microcystis aeruginosa during blooms in 12 lakes spanning four continents as an initial test of the hypothesized Microcystis interactome. We predicted that microbiome composition and functional potential would be similar across blooms globally. Our results, as revealed by 16S rRNA sequence similarity, indicate that M. aeruginosa is cosmopolitan in lakes across a 280 degrees longitudinal and 90 degrees latitudinal gradient. The microbiome communities were represented by a wide range of operational taxonomic units and relative abundances. Highly abundant taxa were more related and shared across most sites and did not vary with geographic distance, thus, like Microcystis, revealing no evidence for dispersal limitation. High phylogenetic relatedness, both within and across lakes, indicates that microbiome bacteria with similar functional potential were associated with all blooms. While Microcystis and the microbiome bacteria shared many genes, whole-community metagenomic analysis revealed a suite of biochemical pathways that could be considered complementary. Our results demonstrate a high degree of similarity across global Microcystis blooms, thereby providing initial support for the hypothesized Microcystis interactome. Y1 - 2019 U6 - https://doi.org/10.1002/lno.11361 SN - 0024-3590 SN - 1939-5590 VL - 65 SP - S194 EP - S207 PB - Wiley CY - Hoboken ER -