TY  - JOUR
A1  - Halilbasic, Emina
A1  - Fuerst, Elisabeth
A1  - Heiden, Denise
A1  - Japtok, Lukasz
A1  - Diesner, Susanne C.
A1  - Trauner, Michael
A1  - Kulu, Askin
A1  - Jaksch, Peter
A1  - Hoetzenecker, Konrad
A1  - Kleuser, Burkhard
A1  - Kazemi-Shirazi, Lili
A1  - Untersmayr, Eva
T1  - Plasma levels of the bioactive sphingolipid metabolite S1P in adult cystic fibrosis patients
BT  - potential target for immunonutrition?
JF  - Nutrients
N2  - Recent research has linked sphingolipid (SL) metabolism with cystic fibrosis transmembrane conductance regulator (CFTR) activity, affecting bioactive lipid mediator sphingosine-1-phosphate (S1P). We hypothesize that loss of CFTR function in cystic fibrosis (CF) patients influenced plasma S1P levels. Total and unbound plasma S1P levels were measured in 20 lung-transplanted adult CF patients and 20 healthy controls by mass spectrometry and enzyme-linked immunosorbent assay (ELISA). S1P levels were correlated with CFTR genotype, routine laboratory parameters, lung function and pathogen colonization, and clinical symptoms. Compared to controls, CF patients showed lower unbound plasma S1P, whereas total S1P levels did not differ. A positive correlation of total and unbound S1P levels was found in healthy controls, but not in CF patients. Higher unbound S1P levels were measured in Delta F508-homozygous compared to Delta F508-heterozygous CF patients (p = 0.038), accompanied by higher levels of HDL in Delta F508-heterozygous patients. Gastrointestinal symptoms were more common in Delta F508 heterozygotes compared to Delta F508 homozygotes. This is the first clinical study linking plasma S1P levels with CFTR function and clinical presentation in adult CF patients. Given the emerging role of immunonutrition in CF, our study might pave the way for using S1P as a novel biomarker and nutritional target in CF.
KW  - sphingolipids
KW  - sphingosine-1-phosphate
KW  - intestine
KW  - high density
KW  - lipoproteins
KW  - cystic fibrosis
KW  - Delta F508 mutation
KW  - immunonutrition
Y1  - 2020
U6  - https://doi.org/10.3390/nu12030765
SN  - 2072-6643
VL  - 12
IS  - 3
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Kyriakos, Konstantinos
A1  - Philipp, Martine
A1  - Adelsberger, Joseph
A1  - Jaksch, Sebastian
A1  - Berezkin, Anatoly V.
A1  - Lugo, Dersy M.
A1  - Richtering, Walter
A1  - Grillo, Isabelle
A1  - Miasnikova, Anna
A1  - Laschewsky, André
A1  - Müller-Buschbaum, Peter
A1  - Papadakis, Christine M.
T1  - Cononsolvency of water/methanol mixtures for PNIPAM and PS-b-PNIPAM: pathway of aggregate formation investigated using time-resolved SANS
JF  - Macromolecules : a publication of the American Chemical Society
N2  - We investigate the cononsolvency effect of poly(N-isopropylacrylamide) (PNIPAM) in mixtures of water and methanol. Two systems are studied: micellar solutions of polystyrene-b-poly(N-isopropylacrylamide) (PS-b-PNIPAM) diblock copolymers and, as a reference, solutions of PNIPAM homopolymers, both at a concentration of 20 mg/mL in DO. Using a stopped-flow instrument, fully deuterated methanol was rapidly added to these solutions at volume fractions between 10 and 20%. Time-resolved turbidimetry revealed aggregate formation within 10-100 s. The structural changes on mesoscopic length scales were followed by time-resolved small-angle neutron scattering (TR-SANS) with a time resolution of 0.1 s. In both systems, the pathway of the aggregation depends on the content of deuterated methanol; however, it is fundamentally different for homopolymer and diblock copolymer solutions: In the former, very large aggregates (>150 nm) are formed within the dead time of the setup, gradient appears at their surface in the late stages. In contrast, the growth of the aggregates in the latter system features different regimes, and the final aggregate size is 50 nm, thus much smaller than for the homopolymer. For the diblock copolymer, the time dependence of the aggregate radius can be described by two models: In the initial stage, the diffusion-limited coalescence model describes the data well; however, the resulting coalescence time is unreasonably high. In the late stage, a logarithmic coalescence model based on an energy barrier which is proportional to the aggregate radius is successfully applied. and a concentration
Y1  - 2014
U6  - https://doi.org/10.1021/ma501434e
SN  - 0024-9297
SN  - 1520-5835
VL  - 47
IS  - 19
SP  - 6867
EP  - 6879
PB  - American Chemical Society
CY  - Washington
ER  -