TY - JOUR A1 - Rein, Julia A1 - Zimmermann, Bernhard A1 - Hille, Carsten A1 - Lang, Ingo A1 - Walz, Bernd A1 - Baumann, Otto T1 - Fluorescence measurements of serotonin-induced V-ATPase-dependent pH changes at the luminal surface in salivary glands of the blowfly Calliphora vicina N2 - Secretion in blowfly salivary glands is induced by the neurohormone serotonin and powered by a vacuolar-type H+- ATPase (V-ATPase) located in the apical membrane of the secretory cells. We have established a microfluorometric method for analysing pH changes at the luminal surface of the secretory epithelial cells by using the fluorescent dye 5-N- hexadecanoyl-aminofluorescein (HAF). After injection of HAF into the lumen of the tubular salivary gland, the fatty acyl chain of the dye molecule partitions into the outer leaflet of the plasma membrane and its pH-sensitive fluorescent moiety is exposed at the cell surface. Confocal imaging has confirmed that HAF distributes over the entire apical membrane of the secretory cells and remains restricted to this membrane domain. Ratiometric analysis of HAF fluorescence demonstrates that serotonin leads to a reversible dose-dependent acidification at the luminal surface. Inhibition by concanamycin A confirms that the serotonin-induced acidification at the luminal surface is due to H+ transport across the apical membrane via V-ATPase. Measurements with pH-sensitive microelectrodes corroborate a serotonin-induced luminal acidification and demonstrate that luminal pH decreases by about 0.4 pH units at saturating serotonin concentrations. We conclude that ratiometric measurements of HAF fluorescence provide an elegant method for monitoring V-ATPase-dependent H+ transport in the blowfly salivary gland in vivo and for analysing the spatiotemporal pattern of pH changes at the luminal surface Y1 - 2006 UR - http://jeb.biologists.org/ U6 - https://doi.org/10.1242/Jeb.02187 SN - 0022-0949 ER - TY - JOUR A1 - Birkhofer, Klaus A1 - Schöning, Ingo A1 - Alt, Fabian A1 - Herold, Nadine A1 - Klarner, Bernhard A1 - Maraun, Mark A1 - Marhan, Sven A1 - Oelmann, Yvonne A1 - Wubet, Tesfaye A1 - Yurkov, Andrey A1 - Begerow, Dominik A1 - Berner, Doreen A1 - Buscot, Francois A1 - Daniel, Rolf A1 - Diekötter, Tim A1 - Ehnes, Roswitha B. A1 - Erdmann, Georgia A1 - Fischer, Christiane A1 - Fösel, Baerbel A1 - Groh, Janine A1 - Gutknecht, Jessica A1 - Kandeler, Ellen A1 - Lang, Christa A1 - Lohaus, Gertrud A1 - Meyer, Annabel A1 - Nacke, Heiko A1 - Näther, Astrid A1 - Overmann, Jörg A1 - Polle, Andrea A1 - Pollierer, Melanie M. A1 - Scheu, Stefan A1 - Schloter, Michael A1 - Schulze, Ernst-Detlef A1 - Schulze, Waltraud X. A1 - Weinert, Jan A1 - Weisser, Wolfgang W. A1 - Wolters, Volkmar A1 - Schrumpf, Marion T1 - General relationships between abiotic soil properties and soil biota across spatial scales and different land-use types JF - PLoS one N2 - Very few principles have been unraveled that explain the relationship between soil properties and soil biota across large spatial scales and different land-use types. Here, we seek these general relationships using data from 52 differently managed grassland and forest soils in three study regions spanning a latitudinal gradient in Germany. We hypothesize that, after extraction of variation that is explained by location and land-use type, soil properties still explain significant proportions of variation in the abundance and diversity of soil biota. If the relationships between predictors and soil organisms were analyzed individually for each predictor group, soil properties explained the highest amount of variation in soil biota abundance and diversity, followed by land-use type and sampling location. After extraction of variation that originated from location or land-use, abiotic soil properties explained significant amounts of variation in fungal, meso-and macrofauna, but not in yeast or bacterial biomass or diversity. Nitrate or nitrogen concentration and fungal biomass were positively related, but nitrate concentration was negatively related to the abundances of Collembola and mites and to the myriapod species richness across a range of forest and grassland soils. The species richness of earthworms was positively correlated with clay content of soils independent of sample location and land-use type. Our study indicates that after accounting for heterogeneity resulting from large scale differences among sampling locations and land-use types, soil properties still explain significant proportions of variation in fungal and soil fauna abundance or diversity. However, soil biota was also related to processes that act at larger spatial scales and bacteria or soil yeasts only showed weak relationships to soil properties. We therefore argue that more general relationships between soil properties and soil biota can only be derived from future studies that consider larger spatial scales and different land-use types. Y1 - 2012 U6 - https://doi.org/10.1371/journal.pone.0043292 SN - 1932-6203 VL - 7 IS - 8 PB - PLoS CY - San Fransisco ER - TY - JOUR A1 - Lang, Ingo A1 - Walz, Bernd T1 - Dopamine-induced epithelial K+ and Na+ movements in the salivary ducts of Periplaneta americana Y1 - 2001 ER - TY - JOUR A1 - Lang, Ingo A1 - Walz, Bernd T1 - Dye-coupling between cells of the salivary glands in the cockroach Periplaneta americana Y1 - 1999 ER - TY - JOUR A1 - Lang, Ingo A1 - Martens, Holger T1 - Na transport in sheep rumen is modulated by voltage-dependent cation conductance in apical membrane Y1 - 1999 ER - TY - JOUR A1 - Schweigel, Monika A1 - Lang, Ingo A1 - Martens, Holger T1 - Mg2+transport of sheep rumen epithelium : evidence for an electrodiffusive uptake mechanism Y1 - 1999 ER - TY - JOUR A1 - Lang, Ingo A1 - Walz, Bernd T1 - Dopamine stimulates salivary duct cells in the cockroach Pertiplaneta americana Y1 - 1999 ER -