TY - JOUR A1 - Gilles, Maria A1 - Otto, Henrike A1 - Wolf, Isabell A. C. A1 - Scharnholz, Barbara A1 - Peus, Verena A1 - Schredl, Michael A1 - Suetterlin, Marc W. A1 - Witt, Stephanie H. A1 - Rietschel, Marcella A1 - Laucht, Manfred A1 - Deuschle, Michael T1 - Maternal hypothalamus-pituitary-adrenal (HPA) system activity and stress measures at birth JF - Psychoneuroendocrinology N2 - Background: Prenatal maternal stress might be a risk for the developing fetus and may have long-lasting effects on child and adult vulnerability to somatic and psychiatric disease. Over-exposure of the unborn to excess glucocorticoids and subsequent alteration of fetal development is hypothesized to be one of the key mechanisms linking prenatal stress with negative child outcome. Methods: In this prospective longitudinal study, mothers-to-be (n = 405) in late pregnancy (36.8 +/- 1.9 weeks of gestational age) and their singleton neonates were studied. We investigated the impact of different prenatal stress indices derived from six stress variables (perceived stress, specific prenatal worries, negative life events, symptoms of depression, trait anxiety, neuroticism) and diurnal maternal saliva cortisol secretion on gestational age and anthropometric measures at birth. KW - Early life stress KW - Gestational age KW - Anthropometric measures at birth KW - Cortisol KW - Prenatal distress KW - Pregnancy Y1 - 2018 U6 - https://doi.org/10.1016/j.psyneuen.2018.04.022 SN - 0306-4530 VL - 94 SP - 152 EP - 161 PB - Elsevier CY - Oxford ER - TY - JOUR A1 - Schieferdecker, Henrike L. A1 - Pestel, Sabine A1 - Püschel, Gerhard Paul A1 - Götze, Otto T1 - Increase by anaphylatoxin C5a of glucose output in perfused rat liver via prostanoids derived from nonparenchymal cells : direct action of prostaglandins and indirect action of thromboxane A(2) on hepatocytes N2 - In the perfused rat liver the anaphylatoxin C5a enhanced glucose output, reduced flow, and elevated prostanoid overflow. Because hepatocytes (HCs) do not express C5a receptors, the metabolic C5a actions must be indirect, mediated by e.g. prostanoids from Kupffer cells (KCs) and hepatic stellate cells (HSCs), which possess C5a receptors. Surprisingly, the metabolic C5a effects were not only impaired by the prostanoid synthesis inhibitor, indomethacin, but also by the thromboxane A(2) (TXA(2)) receptor antagonist, daltroban, even though HCs do not express TXA(2) receptors. TXA(2) did not induce prostaglandin (PG) or an unknown factor release from KCs or sinusoidal endothelial cells (SECs), which express TXA(2) receptors, because (1) daltroban did neither influence the C5a-induced release of prostanoids from cultured KCs nor the C5a-dependent activation of glycogen phosphorylase in KC/HC cocultures and because (2) the TXA(2) analog, U46619, failed to stimulate prostanoid release from cultured KCs or SECs or to activate glycogen phosphorylase in KC/HC or SEC/HC cocultures. In the perfused liver, Ca(2+)-deprivation inhibited not only flow reduction but also glucose output elicited by C5a to similar extents as daltroban. Similarly, in the absence of extracellular Ca(2+), flow reduction and glucose output induced by U46619 were almost completely prevented, whereas glucose output induced by the directly acting PGF(2alpha) was only slightly lowered. Thus, in the perfused rat liver PGs released after C5a- stimulation from KCs and HSCs directly activated glycogen phosphorylase in HCs, and TXA(2) enhanced glucose output indirectly mainly by causing hypoxia as a result of flow reduction. Y1 - 1999 ER - TY - JOUR A1 - Mahlow, Sebastian A1 - Hejazi, Mahdi A1 - Kuhnert, Franziska A1 - Garz, Andreas A1 - Brust, Henrike A1 - Baumann, Otto A1 - Fettke, Jörg T1 - Phosphorylation of transitory starch by -glucan, water dikinase during starch turnover affects the surface properties and morphology of starch granules JF - New phytologist : international journal of plant science N2 - Glucan, water dikinase (GWD) is a key enzyme of starch metabolism but the physico-chemical properties of starches isolated from GWD-deficient plants and their implications for starch metabolism have so far not been described. Transgenic Arabidopsis thaliana plants with reduced or no GWD activity were used to investigate the properties of starch granules. In addition, using various in vitro assays, the action of recombinant GWD, -amylase, isoamylase and starch synthase 1 on the surface of native starch granules was analysed. The internal structure of granules isolated from GWD mutant plants is unaffected, as thermal stability, allomorph, chain length distribution and density of starch granules were similar to wild-type. However, short glucan chain residues located at the granule surface dominate in starches of transgenic plants and impede GWD activity. A similarly reduced rate of phosphorylation by GWD was also observed in potato tuber starch fractions that differ in the proportion of accessible glucan chain residues at the granule surface. A model is proposed to explain the characteristic morphology of starch granules observed in GWD transgenic plants. The model postulates that the occupancy rate of single glucan chains at the granule surface limits accessibility to starch-related enzymes. KW - Arabidopsis thaliana KW - glucan KW - water dikinase (GWD) KW - sex1-8 KW - starch granule surface KW - starch phosphorylation Y1 - 2014 U6 - https://doi.org/10.1111/nph.12801 SN - 0028-646X SN - 1469-8137 VL - 203 IS - 2 SP - 495 EP - 507 PB - Wiley-Blackwell CY - Hoboken ER -