TY  - JOUR
A1  - Voss, Björn
A1  - Bolhuis, Henk
A1  - Fewer, David P.
A1  - Kopf, Matthias
A1  - Möke, Fred
A1  - Haas, Fabian
A1  - El-Shehawy, Rehab
A1  - Hayes, Paul
A1  - Bergman, Birgitta
A1  - Sivonen, Kaarina
A1  - Dittmann-Thünemann, Elke
A1  - Scanlan, Dave J.
A1  - Hagemann, Martin
A1  - Stal, Lucas J.
A1  - Hess, Wolfgang R.
T1  - Insights into the physiology and ecology of the brackish-water-adapted cyanobacterium nodularia spumigena CCY9414 based on a genome-transcriptome analysis
JF  - PLoS one
N2  - Nodularia spumigena is a filamentous diazotrophic cyanobacterium that dominates the annual late summer cyanobacterial blooms in the Baltic Sea. But N. spumigena also is common in brackish water bodies worldwide, suggesting special adaptation allowing it to thrive at moderate salinities. A draft genome analysis of N. spumigena sp. CCY9414 yielded a single scaffold of 5,462,271 nucleotides in length on which genes for 5,294 proteins were annotated. A subsequent strand-specific transcriptome analysis identified more than 6,000 putative transcriptional start sites (TSS). Orphan TSSs located in intergenic regions led us to predict 764 non-coding RNAs, among them 70 copies of a possible retrotransposon and several potential RNA regulators, some of which are also present in other N2-fixing cyanobacteria. Approximately 4% of the total coding capacity is devoted to the production of secondary metabolites, among them the potent hepatotoxin nodularin, the linear spumigin and the cyclic nodulapeptin. The transcriptional complexity associated with genes involved in nitrogen fixation and heterocyst differentiation is considerably smaller compared to other Nostocales. In contrast, sophisticated systems exist for the uptake and assimilation of iron and phosphorus compounds, for the synthesis of compatible solutes, and for the formation of gas vesicles, required for the active control of buoyancy. Hence, the annotation and interpretation of this sequence provides a vast array of clues into the genomic underpinnings of the physiology of this cyanobacterium and indicates in particular a competitive edge of N. spumigena in nutrient-limited brackish water ecosystems.
Y1  - 2013
U6  - https://doi.org/10.1371/journal.pone.0060224
SN  - 1932-6203
VL  - 8
IS  - 3
PB  - PLoS
CY  - San Fransisco
ER  - 
TY  - JOUR
A1  - Duncan, Niall W.
A1  - Hayes, Dave J.
A1  - Wiebking, Christine
A1  - Tiret, Brice
A1  - Pietruska, Karin
A1  - Chen, David Q.
A1  - Rainville, Pierre
A1  - Marjanska, Malgorzata
A1  - Ayad, Omar
A1  - Doyon, Julien
A1  - Hodaie, Mojgan
A1  - Northoff, Georg
T1  - Negative childhood experiences alter a prefrontal-insular-motor cortical network in healthy adults: A preliminary multimodal rsfMRI-fMRI-MRS-dMRI study
JF  - Human brain mapping : a journal devoted to functional neuroanatomy and neuroimaging
N2  - Research in humans and animals has shown that negative childhood experiences (NCE) can have long-term effects on the structure and function of the brain. Alterations have been noted in grey and white matter, in the brain's resting state, on the glutamatergic system, and on neural and behavioural responses to aversive stimuli. These effects can be linked to psychiatric disorder such as depression and anxiety disorders that are influenced by excessive exposure to early life stressors. The aim of the current study was to investigate the effect of NCEs on these systems. Resting state functional MRI (rsfMRI), aversion task fMRI, glutamate magnetic resonance spectroscopy (MRS), and diffusion magnetic resonance imaging (dMRI) were combined with the Childhood Trauma Questionnaire (CTQ) in healthy subjects to examine the impact of NCEs on the brain. Low CTQ scores, a measure of NCEs, were related to higher resting state glutamate levels and higher resting state entropy in the medial prefrontal cortex (mPFC). CTQ scores, mPFC glutamate and entropy, correlated with neural BOLD responses to the anticipation of aversive stimuli in regions throughout the aversion-related network, with strong correlations between all measures in the motor cortex and left insula. Structural connectivity strength, measured using mean fractional anisotropy, between the mPFC and left insula correlated to aversion-related signal changes in the motor cortex. These findings highlight the impact of NCEs on multiple inter-related brain systems. In particular, they highlight the role of a prefrontal-insular-motor cortical network in the processing and responsivity to aversive stimuli and its potential adaptability by NCEs. Hum Brain Mapp 36:4622-4637, 2015. (c) 2015 Wiley Periodicals, Inc.
KW  - affect
KW  - early life stress
KW  - brain networks
KW  - aversion
KW  - resting state
KW  - mood disorder
Y1  - 2015
U6  - https://doi.org/10.1002/hbm.22941
SN  - 1065-9471
SN  - 1097-0193
VL  - 36
IS  - 11
SP  - 4622
EP  - 4637
PB  - Wiley-Blackwell
CY  - Hoboken
ER  -