TY - JOUR A1 - Xie, J. A1 - Techritz, S. A1 - Haebel, Sophie A1 - Horn, A. A1 - Neitzel, H. A1 - Klose, J. A1 - Schuelke, M. T1 - A two-dimensional electrophoretic map of human mitochondrial proteins from immortalized lymphoblastoid cell lines: a prerequisite to study mitochondrial disorders in patients N2 - Mitochondrial diseases may be caused by numerous mutations that alter proteins of the respiratory chain and of other metabolic pathways in the mitochondrium. For clinicians this disease group poses a considerable diagnostic challenge due to ambiguous genotype-phenotype relationships. Until now, only 30 % of the mitochondriopathies can be diagnosed at the molecular level. We therefore need a new diagnostic tool that offers a wide view on the mitochondrial proteins. Here, we present a method to generate a high-resolution, large-gel two-dimensional gel electrophoretic (2-DE) map of a purified fraction of mitochondrial proteins from Epstein-Barr virus-immortalized lymphoblastoid cell line (LCL). LCLs can be easily obtained from patients and control subjects in a routine clinical setting. They often express the biochemical phenotype and can be cultured to high cell numbers, sufficient to gain enough purified material for 2- DE. In total we identified 166 mitochondrial proteins. Thirteen proteins were earlier not known to be of mitochondrial origin. Thirty-nine proteins were associated with human diseases ranging from respiratory chain enzyme deficiencies to disorders of P-oxidation and amino acid metabolism. This 2-DE map is intended to be the first step to diagnose mitochondrial diseases at the proteomic level Y1 - 2005 SN - 1615-9853 ER - TY - JOUR A1 - Mendez-Rebolledo, Guillermo A1 - Gatica-Rojas, Valeska A1 - Martinez-Valdes, Eduardo Andrés A1 - Xie, H. B. T1 - The recruitment order of scapular muscles depends on the characteristics of the postural task JF - Journal of electromyography and kinesiology N2 - Previous studies show that the scapular muscle recruitment order could possibly change according to the characteristics of the postural task. We aimed to compare the activation latencies of serratus anterior (SA), upper, middle, and lower trapezius (UT, MT and LT, respectively) between an unpredictable perturbation (sudden arm destabilization) and a predictable task (voluntary arm raise) and, to determine the differences in the muscle recruitment order in each task. The electromyographic signals of 23 participants were recorded while the tasks were performed. All scapular muscles showed earlier onset latency in the voluntary arm raise than in the sudden arm destabilization. No significant differences were observed in the muscle recruitment order for the sudden arm destabilization (p > 0.05). Conversely, for voluntary arm raise the MT, LT SA and anterior deltoid (AD) were activated significantly earlier than the UT (p < 0.001). Scapular muscles present a specific recruitment order during a predictable task: SA was activated prior to the AD and the UT after the AD, in a recruitment order of SA, AD, UT, MT, and LT. While in an unpredictable motor task, all muscles were activated after the destabilization without a specific recruitment order, but rather a simultaneous activation. (C) 2016 Elsevier Ltd. All rights reserved. KW - Reaction time KW - Muscle latency KW - Electromyography KW - Motor control KW - Anticipatory KW - Compensatory Y1 - 2016 U6 - https://doi.org/10.1016/j.jelekin.2016.09.001 SN - 1050-6411 SN - 1873-5711 VL - 31 SP - 40 EP - 47 PB - Elsevier CY - Oxford ER - TY - JOUR A1 - Kröner, A. A1 - Alexeiev, D. V. A1 - Kovach, V. P. A1 - Rojas-Agramonte, Y. A1 - Tretyakov, A. A. A1 - Mikolaichuk, A. V. A1 - Xie, H. A1 - Sobel, Edward T1 - Zircon ages, geochemistry and Nd isotopic systematics for the Palaeoproterozoic 2.3-1.8 Ga Kuilyu Complex, East Kyrgyzstan BT - the oldest continental basement fragment in the Tianshan orogenic belt JF - Journal of Asian earth sciences N2 - Precambrian microcontinents represent key tectonic units in the accretionary collages of the western Central Asian Orogenic Belt (CAOB), and their geological history is reasonably well established since the Mesoproterozoic but remains weakly constrained for older epochs due to a scarcity of exposed Palaeoproterozoic and Archaean rocks. Early Precambrian rocks were previously reported from several metamorphic complexes in the Kyrgyz Tianshan orogenic belt, mainly based on multigrain conventional zircon dating, but the present study only confirmed such rocks at one site, namely in the Kuilyu Complex of eastern Kyrgyzstan. New single grain SHRIMP II zircon ages, geochemical data, and whole-rock Nd isotopic compositions for granitoid gneisses of the Kuilyu Complex elucidate the age, origin and tectonic settings of this oldest continental fragment in the Tianshan. The Kuilyu Complex is part of the basement in the Ishim - Middle Tianshan microcontinent. It consist of a strongly deformed and metamorphosed supracrustal assemblage of paragneisses and schists which are tectonically interlayered with amphibolites, migmatites and granitoid gneisses. Our zircon dating indicates that the Kuilyu Complex contains two suites of Palaeoproterozoic granitoid gneisses with magmatic protolith ages of ca. 2.32-2.33 Ga and 1.85 Ga. Granitoid magmatism at 1.85 Ga was almost immediately followed by amphibolite-facies metamorphism at ca 1.83 Ga, evidenced by growth of metamorphic zircon rims. The older, ca 2.3 Ga granitoid gneisses chemically correspond to calc-alkaline, metaluminous, I-type magnesian quartz diorite and granodiorite. The protolith of the younger, ca. 1.85 Ga granite-gneiss is an alkalic-calcic, metaluminous to peraluminous, ferroan medium-grained porphyric granite with chemical features resembling A-type granites. The 2.3 Ga and 1.85 Ga granitoid gneisses have slightly to distinctly negative initial epsilon(Nd) values of -1.2 and -6.6, and similar depleted mantle Nd model ages of 2.7-2.6 Ga, which imply melting of Neoarchaean continental crust. The zircon age patterns of the Kuilyu Complex resemble those of exposed rocks in the Tarim Craton, where episodes of granitoid magmatism at ca. 2.3-2.4 and 1.85 Ga, followed by amphibolite-facies metamorphism at ca 1.85 Ga, are also recorded. Similarities in the early Precambrian magmatic and metamorphic episodes as well as similar histories during the Neoproterozoic and early Palaeozoic suggest that the Ishim-Middle Tianshan microcontinent was rifted off the Tarim Craton. Similar age patterns also suggest possible tectonic links of the Kuilyu and Tarim continental blocks with the Baidrag Block of central Mongolia. In contrast, substantial differences in age and Precambrian evolution between the Anrakhai block of southern Kazakhstan and the Kuilyu Complex argue against a previous connection and suggest the former to represent an independent continental terrane. Current data show that early Precambrian rocks in the western CAOB outside Tarim only occur at two sites, namely in the Anrakhai Complex of southern Kazakhstan and in the Kuilyu Complex of eastern Kyrgyzstan. (C) 2016 Elsevier Ltd. All rights reserved. KW - Kuilyu Complex KW - Zircon dating KW - Microcontinent KW - CAOB KW - Middle Tianshan KW - Kyrgyzstan Y1 - 2017 U6 - https://doi.org/10.1016/j.jseaes.2016.12.022 SN - 1367-9120 SN - 1878-5786 VL - 135 SP - 122 EP - 135 PB - Elsevier CY - Oxford ER - TY - GEN A1 - Xie, Chao A1 - Jia, Tianye A1 - Rolls, Edmund T. A1 - Robbins, Trevor W. A1 - Sahakian, Barbara J. A1 - Zhang, Jie A1 - Liu, Zhaowen A1 - Cheng, Wei A1 - Luo, Qiang A1 - Zac Lo, Chun-Yi A1 - Schumann, Gunter A1 - Feng, Jianfeng A1 - Wang, He A1 - Banaschewski, Tobias A1 - Barker, Gareth J. A1 - Bokde, Arun L.W. A1 - Büchel, Christian A1 - Quinlan, Erin Burke A1 - Desrivières, Sylvane A1 - Flor, Herta A1 - Grigis, Antoine A1 - Garavan, Hugh A1 - Gowland, Penny A1 - Heinz, Andreas A1 - Hohmann, Sarah A1 - Ittermann, Bernd A1 - Martinot, Jean-Luc A1 - Paillère Martinot, Marie-Laure A1 - Nees, Frauke A1 - Papadopoulos Orfanos, Dimitri A1 - Paus, Tomáš A1 - Poustka, Luise A1 - Fröhner, Juliane H. A1 - Smolka, Michael N. A1 - Walter, Henrik A1 - Whelan, Robert T1 - Reward versus nonreward sensitivity of the medial versus lateral orbitofrontal cortex relates to the severity of depressive symptoms T2 - Zweitveröffentlichungen der Universität Potsdam : Humanwissenschaftliche Reihe N2 - BACKGROUND: The orbitofrontal cortex (OFC) is implicated in depression. The hypothesis investigated was whether the OFC sensitivity to reward and nonreward is related to the severity of depressive symptoms. METHODS: Activations in the monetary incentive delay task were measured in the IMAGEN cohort at ages 14 years (n = 1877) and 19 years (n = 1140) with a longitudinal design. Clinically relevant subgroups were compared at ages 19 (high-severity group: n = 116; low-severity group: n = 206) and 14. RESULTS: The medial OFC exhibited graded activation increases to reward, and the lateral OFC had graded activation increases to nonreward. In this general population, the medial and lateral OFC activations were associated with concurrent depressive symptoms at both ages 14 and 19 years. In a stratified high-severity depressive symptom group versus control group comparison, the lateral OFC showed greater sensitivity for the magnitudes of activations related to nonreward in the high-severity group at age 19 (p = .027), and the medial OFC showed decreased sensitivity to the reward magnitudes in the high-severity group at both ages 14 (p = .002) and 19 (p = .002). In a longitudinal design, there was greater sensitivity to nonreward of the lateral OFC at age 14 for those who exhibited high depressive symptom severity later at age 19 (p = .003). CONCLUSIONS: Activations in the lateral OFC relate to sensitivity to not winning, were associated with high depressive symptom scores, and at age 14 predicted the depressive symptoms at ages 16 and 19. Activations in the medial OFC were related to sensitivity to winning, and reduced reward sensitivity was associated with concurrent high depressive symptom scores. T3 - Zweitveröffentlichungen der Universität Potsdam : Humanwissenschaftliche Reihe - 860 KW - adolescents KW - depression KW - monetary incentive delay task KW - nonreward sensitivity KW - orbitofrontal cortex KW - reward anticipation KW - reward sensitivity KW - ventral striatum Y1 - 2021 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-557882 SN - 1866-8364 IS - 3 ER - TY - JOUR A1 - Xie, Chao A1 - Jia, Tianye A1 - Rolls, Edmund T. A1 - Robbins, Trevor W. A1 - Sahakian, Barbara J. A1 - Zhang, Jie A1 - Liu, Zhaowen A1 - Cheng, Wei A1 - Luo, Qiang A1 - Zac Lo, Chun-Yi A1 - Schumann, Gunter A1 - Feng, Jianfeng A1 - Wang, He A1 - Banaschewski, Tobias A1 - Barker, Gareth J. A1 - Bokde, Arun L.W. A1 - Büchel, Christian A1 - Quinlan, Erin Burke A1 - Desrivières, Sylvane A1 - Flor, Herta A1 - Grigis, Antoine A1 - Garavan, Hugh A1 - Gowland, Penny A1 - Heinz, Andreas A1 - Hohmann, Sarah A1 - Ittermann, Bernd A1 - Martinot, Jean-Luc A1 - Paillère Martinot, Marie-Laure A1 - Nees, Frauke A1 - Papadopoulos Orfanos, Dimitri A1 - Paus, Tomáš A1 - Poustka, Luise A1 - Fröhner, Juliane H. A1 - Smolka, Michael N. A1 - Walter, Henrik A1 - Whelan, Robert T1 - Reward versus nonreward sensitivity of the medial versus lateral orbitofrontal cortex relates to the severity of depressive symptoms JF - Biological Psychiatry: Cognitive Neuroscience and Neuroimaging N2 - BACKGROUND: The orbitofrontal cortex (OFC) is implicated in depression. The hypothesis investigated was whether the OFC sensitivity to reward and nonreward is related to the severity of depressive symptoms. METHODS: Activations in the monetary incentive delay task were measured in the IMAGEN cohort at ages 14 years (n = 1877) and 19 years (n = 1140) with a longitudinal design. Clinically relevant subgroups were compared at ages 19 (high-severity group: n = 116; low-severity group: n = 206) and 14. RESULTS: The medial OFC exhibited graded activation increases to reward, and the lateral OFC had graded activation increases to nonreward. In this general population, the medial and lateral OFC activations were associated with concurrent depressive symptoms at both ages 14 and 19 years. In a stratified high-severity depressive symptom group versus control group comparison, the lateral OFC showed greater sensitivity for the magnitudes of activations related to nonreward in the high-severity group at age 19 (p = .027), and the medial OFC showed decreased sensitivity to the reward magnitudes in the high-severity group at both ages 14 (p = .002) and 19 (p = .002). In a longitudinal design, there was greater sensitivity to nonreward of the lateral OFC at age 14 for those who exhibited high depressive symptom severity later at age 19 (p = .003). CONCLUSIONS: Activations in the lateral OFC relate to sensitivity to not winning, were associated with high depressive symptom scores, and at age 14 predicted the depressive symptoms at ages 16 and 19. Activations in the medial OFC were related to sensitivity to winning, and reduced reward sensitivity was associated with concurrent high depressive symptom scores. KW - adolescents KW - depression KW - monetary incentive delay task KW - nonreward sensitivity KW - orbitofrontal cortex KW - reward anticipation KW - reward sensitivity KW - ventral striatum Y1 - 2021 U6 - https://doi.org/10.1016/j.bpsc.2020.08.017 SN - 2451-9022 SN - 2451-9030 VL - 6 IS - 3 SP - 259 EP - 269 PB - Elsevier Science CY - Amsterdam ER -