TY  - JOUR
A1  - Morgner, Frank
A1  - Bennemann, Mark
A1  - Cywiński, Piotr J.
A1  - Kollosche, Matthias
A1  - Górski, Krzysztof
A1  - Pietraszkiewicz, Marek
A1  - Geßner, André
A1  - Löhmannsröben, Hans-Gerd
T1  - Elastic FRET sensors for contactless pressure measurement
JF  - RSC Advances : an international journal to further the chemical sciences
N2  - Contactless pressure monitoring based on Forster resonance energy transfer between donor/acceptor pairs immobilized within elastomers is demonstrated. The donor/acceptor energy transfer is employed by dispersing terbium(III) tris[(2-hydroxybenzoyl)-2-aminoethyl] amine complex (LLC, donor) and CdSe/ZnS quantum dots (QD655, acceptor) in styrene-ethylene/buthylene-styrene (SEBS) and poly(dimethylsiloxane) (PDMS). The continuous monitoring of QD luminescence showed a reversible intensity change as the pressure signal is alternated between two stable states indicating a pressure sensitivity of 6350 cps kPa(-1). Time-resolved measurements show the pressure impact on the FRET signal due to an increase of decay time (270 ms up to 420 ms) for the donor signal and parallel drop of decay time (170 mu s to 155 mu s) for the acceptor signal as the net pressure applied. The LLC/QD655 sensors enable a contactless readout as well as space resolved monitoring to enable miniaturization towards smaller integrated stretchable opto-electronics. Elastic FRET sensors can potentially lead to developing profitable analysis systems capable to outdo conventional wired electronic systems (inductive, capacitive, ultrasonic and photoelectric sensors) especially for point-of-care diagnostics, biological monitoring required for wearable electronics.
Y1  - 2017
U6  - https://doi.org/10.1039/c7ra06379b
SN  - 2046-2069
VL  - 7
SP  - 50578
EP  - 50583
PB  - RSC Publishing
CY  - Cambridge
ER  - 
TY  - JOUR
A1  - Cywinski, Piotr J.
A1  - Pietraszkiewicz, Marek
A1  - Maciejczyk, Michal
A1  - Gorski, Krzysztof
A1  - Hammann, Tommy
A1  - Liermann, Konstanze
A1  - Paulke, Bernd-Reiner
A1  - Löhmannsröben, Hans-Gerd
T1  - Total protein concentration quantification using nanobeads with a new highly luminescent terbium(III) complex
JF  - RSC Advances
N2  - Total protein concentration (TPC) is a key parameter in many biochemical experiments and its quantification is often necessary for isolation, separation, and analysis of proteins. A sensitive and rapid nanobead-based TPC quantification assay based on Forster Resonance Energy Transfer (FRET) has been developed. A new, highly luminescent Tb(III) complex has been synthesised and applied as donor in this FRET assay with an organic dye (Cy5) as acceptor. FRET-induced changes in luminescence have been investigated both at donor and acceptor emission wavelength using time-resolved luminescence spectroscopy with time-gated detection. In the assay, the Tb(III) complex and fine-tuned polyglycidyl methacrylate (PGMA) nanobeads ensure that an improvement in sensitivity and background reduction is achieved. Using 40 nm large PGMA nanobeads loaded with the Tb(III) complex, it is possible to determine TPC down to 50 ng mL(-1) in just 10 minutes. Through specific assay components the sensitivity has been improved when compared to existing nanobead-based assays and to currently known commercial methods. Additionally, the assay is relatively insensitive to the presence of contaminants, such as non-ionic detergents commonly found in biological samples. Due to no need for any centrifugal steps, this mix-and-measure bioassay can easily be implemented into routine TPC quantification protocols in biochemical laboratories.
Y1  - 2016
U6  - https://doi.org/10.1039/c6ra23207h
SN  - 2046-2069
VL  - 6
SP  - 115068
EP  - 115073
PB  - Royal Society of Chemistry
CY  - Cambridge
ER  -