TY - GEN A1 - Westendorf, Christian A1 - Bae, Albert J. A1 - Erlenkamper, Christoph A1 - Galland, Edouard A1 - Franck, Carl A1 - Bodenschatz, Eberhard A1 - Beta, Carsten T1 - Live cell flattening BT - traditional and novel approaches T2 - Postprints der Universität Potsdam : Mathematisch Naturwissenschaftliche Reihe N2 - Eukaryotic cell flattening is valuable for improving microscopic observations, ranging from bright field (BF) to total internal reflection fluorescence (TIRF) microscopy. Fundamental processes, such as mitosis and in vivo actin polymerization, have been investigated using these techniques. Here, we review the well known agar overlayer protocol and the oil overlay method. In addition, we present more elaborate microfluidics-based techniques that provide us with a greater level of control. We demonstrate these techniques on the social amoebae Dictyostelium discoideum, comparing the advantages and disadvantages of each method. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 835 KW - PDMS KW - microfluidic device KW - lower channel KW - total internal reflection fluorescence KW - total internal reflection fluorescence microscopy Y1 - 2020 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-428311 SN - 1866-8372 IS - 835 ER - TY - JOUR A1 - Miegel, Konrad A1 - Graeff, Thomas A1 - Selle, Benny A1 - Salzmann, Thomas A1 - Franck, Christian A1 - Bronstert, Axel T1 - Investigation of a renatured fen on the Baltic Sea coast of Mecklenburg - Part I: System description and basic hydrological characterisation JF - Hydrologie und Wasserbewirtschaftung KW - Baltic Sea Coast KW - coastal wetland KW - drainage of the catchment area KW - fens KW - groundwater flow KW - management effects KW - system analysis KW - water balance Y1 - 2016 U6 - https://doi.org/10.5675/HyWa_2016.4_1 SN - 1439-1783 VL - 60 SP - 242 EP - 258 PB - Bundesanst. für Gewässerkunde CY - Koblenz ER - TY - JOUR A1 - Miegel, Konrad A1 - Gräff, Thomas A1 - Franck, Christian A1 - Salzmann, Thomas A1 - Bronstert, Axel A1 - Walther, Marc A1 - Oswald, Sascha T1 - Auswirkungen des Sturmhochwassers der Ostsee am 4./5. Januar 2017 auf das renaturierte Nieder- moor „Hütelmoor und Heiligensee“ an der deut- schen Ostseeküste BT - Effects of the January 4/5 2017 storm surge on a restored fen at the German Baltic Sea coast JF - Hydrologie und Wasserbewirtschaftung N2 - Entlang der Küstenniederung des Naturschutzgebietes „Hütelmoor und Heiligensee“, ca. 6 km nordöstlich von Rostock-Warnemünde gelegen, wird seit dem Jahr 2000 die Küstendüne nicht mehr instand gehalten. Im Rahmen der Renaturierung des Gebietes werden so grundsätzlich wieder Überflutungen bei Ostseehochwassern zugelassen, was bisher jedoch noch nicht eingetreten ist. Am 4./5. Januar 2017 ereignete sich ein Sturmhochwasser der Ostsee, mit einem Scheitelwasserstand in Warnemünde, der sich zwischen dem 10- und 20-jährlichen Hochwasserstand einordnet. Dennoch kam es bei diesem Ereignis nicht zum Dünendurchbruch und zur seeseitigen Überflutung, wohl aber zum binnenseitigen Einstrom von Salz- bzw. Brackwasser. Dieser erfolgte über den Graben, durch den das Gebiet normalerweise über die Warnow in die Ostsee entwässert. Durch das Einströmen über die Sohlschwelle, sonst Auslass des Gebietes, stiegen die Wasserstände und Salzkonzentrationen in der südwestlichen Hälfte der Niederung an. Mit zunehmender Entfernung zur Sohlschwelle waren diese Auswirkungen jedoch geringer spürbar. Dies gilt wegen der Retentionswirkung der Niederung mehr für den Wasserstand als für die Salzkonzentration. Während der Wasserstand durch den Einstau der Niederung und Überschwemmungen flächenhaft anstieg, breitete sich die Salzfront präferentiell in den ehemaligen Entwässerungsgräben, die trotz des Einstaus nach wie vor hydraulisch aktiv sind, eher linienhaft aus. Diese Interpretation beruht auf Messergebnissen von Wasserstand, elektrischer Leitfähigkeit und Wassertemperatur. N2 - Maintenance and repair of the coastal dunes of the conservation area, Hutelmoor und Heiligensee" have been ceased for renaturation purposes since the year 2000 to aid flooding of the area during high water levels of the Baltic Sea. On January 4th and 5th 2017, the Baltic Sea experienced a storm surge resulting in a water level in Warnemunde with a 10-to 20-year reoccurrence rate. Nevertheless, the event caused neither a dune failure nor a sea-sided flooding, but did result in an upstream inflow of salt and brackwater through the trench which normally drains the area into the Baltic Sea via the Warnow. Water levels and salinity concentrations rose in the south-western part of the area due to the influx via a ground sill, which normally acts as the outlet of the lowland. The effects within the lowland were less pronounced with increasing distance to the ground sill, which proved to be even more significant for the water levels than for salinities due to the retention capabilities of the area. While water levels increased extensively as a result of the flooding, the salinity front is presumed to have spread primarily along the former draining channels. This interpretation is based on monitoring data of the water level as well as the environmental tracers electrical conductivity and water temperature. KW - Baltic Sea KW - storm surge KW - coastal lowland KW - flooding KW - salinization KW - environmental tracer KW - process analysis KW - Ostsee KW - Sturmhochwasser KW - Küstenniederung KW - Überflutung KW - Versalzung KW - Umwelttracer KW - Prozessgeschehen Y1 - 2017 U6 - https://doi.org/10.5675/HyWa_2017,4_2 SN - 1439-1783 VL - 61 IS - 4 SP - 232 EP - 243 PB - Bundesanst. für Gewässerkunde CY - Koblenz ER - TY - JOUR A1 - Allan, Eric A1 - Weisser, Wolfgang W. A1 - Fischer, Markus A1 - Schulze, Ernst-Detlef A1 - Weigelt, Alexandra A1 - Roscher, Christiane A1 - Baade, Jussi A1 - Barnard, Romain L. A1 - Bessler, Holger A1 - Buchmann, Nina A1 - Ebeling, Anne A1 - Eisenhauer, Nico A1 - Engels, Christof A1 - Fergus, Alexander J. F. A1 - Gleixner, Gerd A1 - Gubsch, Marlen A1 - Halle, Stefan A1 - Klein, Alexandra-Maria A1 - Kertscher, Ilona A1 - Kuu, Annely A1 - Lange, Markus A1 - Le Roux, Xavier A1 - Meyer, Sebastian T. A1 - Migunova, Varvara D. A1 - Milcu, Alexandru A1 - Niklaus, Pascal A. A1 - Oelmann, Yvonne A1 - Pasalic, Esther A1 - Petermann, Jana S. A1 - Poly, Franck A1 - Rottstock, Tanja A1 - Sabais, Alexander C. W. A1 - Scherber, Christoph A1 - Scherer-Lorenzen, Michael A1 - Scheu, Stefan A1 - Steinbeiss, Sibylle A1 - Schwichtenberg, Guido A1 - Temperton, Vicky A1 - Tscharntke, Teja A1 - Voigt, Winfried A1 - Wilcke, Wolfgang A1 - Wirth, Christian A1 - Schmid, Bernhard T1 - A comparison of the strength of biodiversity effects across multiple functions JF - Oecologia N2 - In order to predict which ecosystem functions are most at risk from biodiversity loss, meta-analyses have generalised results from biodiversity experiments over different sites and ecosystem types. In contrast, comparing the strength of biodiversity effects across a large number of ecosystem processes measured in a single experiment permits more direct comparisons. Here, we present an analysis of 418 separate measures of 38 ecosystem processes. Overall, 45 % of processes were significantly affected by plant species richness, suggesting that, while diversity affects a large number of processes not all respond to biodiversity. We therefore compared the strength of plant diversity effects between different categories of ecosystem processes, grouping processes according to the year of measurement, their biogeochemical cycle, trophic level and compartment (above- or belowground) and according to whether they were measures of biodiversity or other ecosystem processes, biotic or abiotic and static or dynamic. Overall, and for several individual processes, we found that biodiversity effects became stronger over time. Measures of the carbon cycle were also affected more strongly by plant species richness than were the measures associated with the nitrogen cycle. Further, we found greater plant species richness effects on measures of biodiversity than on other processes. The differential effects of plant diversity on the various types of ecosystem processes indicate that future research and political effort should shift from a general debate about whether biodiversity loss impairs ecosystem functions to focussing on the specific functions of interest and ways to preserve them individually or in combination. KW - Bottom-up effects KW - Carbon cycling KW - Ecological synthesis KW - Ecosystem processes KW - Grasslands KW - Jena experiment KW - Nitrogen cycling Y1 - 2013 U6 - https://doi.org/10.1007/s00442-012-2589-0 SN - 0029-8549 VL - 173 IS - 1 SP - 223 EP - 237 PB - Springer CY - New York ER -