TY - JOUR A1 - Beye, Martin A1 - Öberg, Henrik A1 - Xin, Hongliang A1 - Dakovski, Georgi L. A1 - Föhlisch, Alexander A1 - Gladh, Jorgen A1 - Hantschmann, Markus A1 - Hieke, Florian A1 - Kaya, Sarp A1 - Kühn, Danilo A1 - LaRue, Jerry A1 - Mercurio, Giuseppe A1 - Minitti, Michael P. A1 - Mitra, Ankush A1 - Moeller, Stefan P. A1 - Ng, May Ling A1 - Nilsson, Anders A1 - Nordlund, Dennis A1 - Norskov, Jens A1 - Öström, Henrik A1 - Ogasawara, Hirohito A1 - Persson, Mats A1 - Schlotter, William F. A1 - Sellberg, Jonas A. A1 - Wolf, Martin A1 - Abild-Pedersen, Frank A1 - Pettersson, Lars G. M. A1 - Wurth, Wilfried T1 - Chemical Bond Activation Observed with an X-ray Laser JF - The journal of physical chemistry letters N2 - The concept of bonding and antibonding orbitals is fundamental in chemistry. The population of those orbitals and the energetic difference between the two reflect the strength of the bonding interaction. Weakening the bond is expected to reduce this energetic splitting, but the transient character of bond-activation has so far prohibited direct experimental access. Here we apply time-resolved soft X-ray spectroscopy at a free electron laser to directly observe the decreased bonding antibonding splitting following bond-activation using an ultrashort optical laser pulse. Y1 - 2016 U6 - https://doi.org/10.1021/acs.jpclett.6b01543 SN - 1948-7185 VL - 7 SP - 3647 EP - 3651 PB - American Chemical Society CY - Washington ER - TY - JOUR A1 - Stede, Manfred A1 - Kuhn, Florian T1 - Identifying the content zones of German court decisions Y1 - 2009 SN - 978-3-642- 03423-7 ER - TY - BOOK A1 - Schroeder, Christoph A1 - Schellhardt, Christin A1 - Akinci, Mehmet-Ali A1 - Dollnick, Meral A1 - Dux, Ginesa A1 - Gülbeyaz, Esin Işıl A1 - Jähnert, Anne A1 - Koç-Gültürk, Ceren A1 - Kühmstedt, Patrick A1 - Kuhn, Florian A1 - Mezger, Verena A1 - Pfaff, Carol A1 - Ürkmez, Betül Sena ED - Schroeder, Christoph ED - Schellhardt, Christin T1 - MULTILIT BT - manual, criteria of transcription and analysis for German, Turkish and English N2 - This paper presents an overview of the linguistic analyses developed in the MULTILIT project and the processing of the oral and written texts collected. The project investigates the language abilities of multilingual children and adolescents, in particular, those who have Turkish and/or Kurdish as a mother tongue. A further aim of the project is to examine from a psycholinguistic and sociolinguistic perspective the extent to which competence in academic registers is achieved on the basis of the languages spoken by the children, including the language(s) spoken at the home, the language of the country of residence and the first foreign language. To be able to examine these questions using corpus linguistic parameters, we created categories of analysis in MULTILIT. The data collection comprises texts from bilingual and monolingual children and adolescents in Germany in their first language Turkish, their second language German und their foreign language English. Pupils aged between nine and twenty years of age produced monologue oral and written texts in the two genres of narrative and discursive. On the basis of these samples, we examine linguistic features such as lexical expression (lexical density, lexical diversity), syntactic complexity (syntactic and discursive packaging) as well as phonology in the oral texts and orthography in the written texts, with the aim of investigating the pupils’ growing mastery of these features in academic and informal registers. To this end the raw data have been transcribed by the use of transcription conventions developed especially for the needs of the MULTILIT data. They are based on the commonly used HIAT and GAT transcription conventions and supplemented with conventions that provide additional information such as features at the graphic level. The categories of analysis comprise a large number of linguistic categories such as word classes, syntax, noun phrase complexity, complex verbal morphology, direct speech and text structures. We also annotate errors and norm deviations at a wide range of levels (orthographic, morphological, lexical, syntactic and textual). In view of the different language systems, these criteria are considered separately for all languages investigated in the project. N2 - Die vorliegende Arbeit stellt eine Übersicht der linguistischen Analysen dar, die im Rahmen des MULTILIT Projektes entwickelt wurden. Darüber hinaus wird die Aufbereitung der erhobenen mündlichen und schriftlichen Texte vorgestellt. Das Projekt betrachtet die sprachlichen Fähigkeiten multilingualer Kinder und Jugendlicher, insbesondere mit der Muttersprache Türkisch und/oder Kurdisch. Ein weiteres Ziel des Projektes ist die Untersuchung der Entwicklung eines akademischen Registers der Sprachen der Kinder, d.h. der zu Hause gesprochenen Sprache(n), der Sprache des Aufenthaltslandes und der ersten Fremdsprache unter psycholinguistischen und soziolinguistischen Gesichtspunkten. Zur Untersuchung dieser Forschungsfragen unter korpuslinguistischen Parametern wurden in MULTILIT Analysekriterien entwickelt. Die Datenerhebung umfasst Texte bilingualer und monolingualer Kinder und Jugendlicher in ihrer Erstsprache Türkisch, ihrer Zweitsprache Deutsch sowie ihrer ersten Fremdsprache Englisch. Schüler im Alter von 9 bis 20 Jahren haben sowohl mündliche als auch schriftliche monologische Texte in zwei Genres produziert – erzählend und erörternd. Basierend auf diese Daten untersuchen wir linguistische Bereiche wie lexikalischer Ausdruck (lexikalische Dichte, lexikalische Vielfalt), syntaktische Komplexität (syntaktische und diskursive Verdichtung) sowie Phonologie in den mündlichen Texten und Orthographie in den schriftlichen Texten mit dem Ziel, die wachsende Beherrschung dieser Bereiche in akademischen und informellen Registern durch die Schüler zu untersuchen. Dafür wurden die Rohdaten mit Transkriptionskonventionen verarbeitet, die speziell auf die Bedürfnisse des MULTILIT Projektes zugeschnitten sind. Sie basieren auf den weit verbreiteten Transkriptionskonventionen HIAT und GAT und wurden durch Konventionen erweitert, die zusätzliche Informationen, beispielsweise auf graphischer Ebene, festhalten. Die Analysekategorien umfassen zahlreiche linguistische Kategorien, wie Wortarten, Syntax, Nominalphrasenkomplexität, komplexe Verbalmorphologie, direkte Rede und Textstrukturen. Außerdem annotieren wir Fehler und Normabweichungen auf allen zahlreichen Ebenen (orthographisch, morphologisch, lexikalisch, syntaktisch und textuell). Aufgrund der verschiedenen Sprachsysteme werden diese Analysekategorien für alle im Projekt untersuchten Sprachen gesondert betrachtet. KW - bilingualism KW - child KW - German KW - German lessons KW - migration KW - multilingualism KW - second language KW - Turkish KW - writing ability KW - written language acquisition KW - DaZ KW - Deutsch KW - Deutschunterricht KW - Kind KW - Mehrsprachigkeit KW - Schreiben KW - Schreibfähigkeit KW - Schriftsprache KW - Schriftspracherwerb KW - Sprachförderung KW - Türkisch KW - Zweitsprache Y1 - 2015 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-80390 ER - TY - GEN A1 - Schäfer, Marjänn Helena A1 - Kakularam, Kumar Reddy A1 - Reisch, Florian A1 - Rothe, Michael A1 - Stehling, Sabine A1 - Heydeck, Dagmar A1 - Püschel, Gerhard Paul A1 - Kuhn, Hartmut T1 - Male Knock-in Mice Expressing an Arachidonic Acid Lipoxygenase 15B (Alox15B) with Humanized Reaction Specificity Are Prematurely Growth Arrested When Aging T2 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe N2 - Mammalian arachidonic acid lipoxygenases (ALOXs) have been implicated in cell differentiation and in the pathogenesis of inflammation. The mouse genome involves seven functional Alox genes and the encoded enzymes share a high degree of amino acid conservation with their human orthologs. There are, however, functional differences between mouse and human ALOX orthologs. Human ALOX15B oxygenates arachidonic acid exclusively to its 15-hydroperoxy derivative (15S-HpETE), whereas 8S-HpETE is dominantly formed by mouse Alox15b. The structural basis for this functional difference has been explored and in vitro mutagenesis humanized the reaction specificity of the mouse enzyme. To explore whether this mutagenesis strategy may also humanize the reaction specificity of mouse Alox15b in vivo, we created Alox15b knock-in mice expressing the arachidonic acid 15-lipoxygenating Tyr603Asp+His604Val double mutant instead of the 8-lipoxygenating wildtype enzyme. These mice are fertile, display slightly modified plasma oxylipidomes and develop normally up to an age of 24 weeks. At later developmental stages, male Alox15b-KI mice gain significantly less body weight than outbred wildtype controls, but this effect was not observed for female individuals. To explore the possible reasons for the observed gender-specific growth arrest, we determined the basic hematological parameters and found that aged male Alox15b-KI mice exhibited significantly attenuated red blood cell parameters (erythrocyte counts, hematocrit, hemoglobin). Here again, these differences were not observed in female individuals. These data suggest that humanization of the reaction specificity of mouse Alox15b impairs the functionality of the hematopoietic system in males, which is paralleled by a premature growth arrest. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1295 KW - eicosanoids KW - lipid peroxidation KW - oxidative stress KW - polyenoic fatty acids KW - erythropoiesis Y1 - 2023 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-576491 SN - 1866-8372 IS - 1295 ER - TY - JOUR A1 - Schäfer, Marjänn Helena A1 - Kakularam, Kumar Reddy A1 - Reisch, Florian A1 - Rothe, Michael A1 - Stehling, Sabine A1 - Heydeck, Dagmar A1 - Püschel, Gerhard Paul A1 - Kuhn, Hartmut T1 - Male Knock-in Mice Expressing an Arachidonic Acid Lipoxygenase 15B (Alox15B) with Humanized Reaction Specificity Are Prematurely Growth Arrested When Aging JF - Biomedicines N2 - Mammalian arachidonic acid lipoxygenases (ALOXs) have been implicated in cell differentiation and in the pathogenesis of inflammation. The mouse genome involves seven functional Alox genes and the encoded enzymes share a high degree of amino acid conservation with their human orthologs. There are, however, functional differences between mouse and human ALOX orthologs. Human ALOX15B oxygenates arachidonic acid exclusively to its 15-hydroperoxy derivative (15S-HpETE), whereas 8S-HpETE is dominantly formed by mouse Alox15b. The structural basis for this functional difference has been explored and in vitro mutagenesis humanized the reaction specificity of the mouse enzyme. To explore whether this mutagenesis strategy may also humanize the reaction specificity of mouse Alox15b in vivo, we created Alox15b knock-in mice expressing the arachidonic acid 15-lipoxygenating Tyr603Asp+His604Val double mutant instead of the 8-lipoxygenating wildtype enzyme. These mice are fertile, display slightly modified plasma oxylipidomes and develop normally up to an age of 24 weeks. At later developmental stages, male Alox15b-KI mice gain significantly less body weight than outbred wildtype controls, but this effect was not observed for female individuals. To explore the possible reasons for the observed gender-specific growth arrest, we determined the basic hematological parameters and found that aged male Alox15b-KI mice exhibited significantly attenuated red blood cell parameters (erythrocyte counts, hematocrit, hemoglobin). Here again, these differences were not observed in female individuals. These data suggest that humanization of the reaction specificity of mouse Alox15b impairs the functionality of the hematopoietic system in males, which is paralleled by a premature growth arrest. KW - eicosanoids KW - lipid peroxidation KW - oxidative stress KW - polyenoic fatty acids KW - erythropoiesis Y1 - 2022 U6 - https://doi.org/10.3390/biomedicines10061379 SN - 2227-9059 VL - 10 SP - 1 EP - 22 PB - MDPI CY - Basel, Schweiz ET - 6 ER -